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The map label for this gene is metZ [H]

Identifier: 209547789

GI number: 209547789

Start: 179370

End: 180554

Strand: Reverse

Name: metZ [H]

Synonym: Rleg2_0180

Alternate gene names: 209547789

Gene position: 180554-179370 (Counterclockwise)

Preceding gene: 209547791

Following gene: 209547788

Centisome position: 3.98

GC content: 62.87

Gene sequence:

>1185_bases
ATGAGCAAGACCTGGCGCCCGGCAACCCAACTCGTCCACGGCGGAACCTTGCGTTCGCAATATGGCGAAACATCCGAGGC
AATCTATCTCACGCAGGGCTTCGTCTATGAGACGTCGGAAGCGGCAGAAGCCCGCTTCAAGGGCGAGACTGAAGGTTACA
TCTACGCCCGCTACGGCAGCCCGACCAACGACATGTTCGAAAAGCGCATGTGCATGCTCGAAGGCGCGGAAGACGCCCGC
GCCACCGCGTCCGGCATGGCGGCGGTCAGCGCAGCCATCCTCTGCCAGGTCAAGGCCGGCGACCATATCGTTGCCGCGCG
CGCCCTCTTCGGCTCCTGCCGCTGGGTGGTGGAAACATTGGCGCCGAAATACGGCGTCGAGTGCACGCTGGTCGACGGCC
GGGATCTTGCCAACTGGGAAAAGGCAATTCGGCCGAACACCAAGGTGTTCTTCCTGGAAAGCCCGACCAACCCGACGCTC
GAGGTGGTCGATATATCAGGCGTCGCCAAGCTCGCCAACCAGATCGGCGCCAAGGTCGTCGTCGACAACGTCTTTGCGAC
GCCACTCTTCCAGAAGCCGTTGGAACTCGGCGCCCATATCGTCGTCTATTCCGCCACCAAGCATATCGACGGCCAGGGCC
GCTGCCTCGGCGGCGTCGTGCTTGCCGACAAGGACTGGATCGACGAGAACCTGCACGACTATTTCCGCCATACCGGCCCG
GCCATGTCGCCGTTCAATGCCTGGACGCTGCTGAAGGGCATCGAAACGCTGCCGCTGCGCGTCAAGCAGCAGACCGGGAA
TGCGGCGAAGATCGCCGATTTCCTCGCCGAGCAGAGCAAGGTCGCCAAGGTGATCTATCCCGGCCGCAAGGACCATCCGC
AGGCCGACATCATCGCCAAGCAGATGACCGGCGGCTCGACCCTCGTCGCCTTCGAATTGAAGGGCGGCAAGGAGGCGGCC
TTCGCGCTGCAGAACGCGCTCGATATCGTCAAGATTTCCAACAATCTCGGCGACAGCAAGAGCCTGATCACCCATCCGGC
AACGACGACGCACAAGAACCTGACGGAAGAGGCGCGCGCCGAACTCGGCATCTCCCCGGGCACGGTCCGCCTTTCGGCCG
GCATCGAGGATACCGACGACCTGATCGAGGATTTCGCCAAGGCGCTTGCCAAGGTCTCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGGTCGGCTTGCAGCCACGTTAAACAAGTGGCTAAAAAGACCGGGTTGAAACCGGTCTGCTTTTGCGGCCGGTTTTTT
TGTTATGTGAAGGTGATGGC

Downstream 100 bases:

>100_bases
TCGCACTAAAGCGCATCGCATCAAATTTGATTCATGCGACGCGCTTTAGCTCTTTGTTTTGATGCATGTCGTTATCCCGG
AACCGCTGCACACTTTCGGG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKTWRPATQLVHGGTLRSQYGETSEAIYLTQGFVYETSEAAEARFKGETEGYIYARYGSPTNDMFEKRMCMLEGAEDAR
ATASGMAAVSAAILCQVKAGDHIVAARALFGSCRWVVETLAPKYGVECTLVDGRDLANWEKAIRPNTKVFFLESPTNPTL
EVVDISGVAKLANQIGAKVVVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLADKDWIDENLHDYFRHTGP
AMSPFNAWTLLKGIETLPLRVKQQTGNAAKIADFLAEQSKVAKVIYPGRKDHPQADIIAKQMTGGSTLVAFELKGGKEAA
FALQNALDIVKISNNLGDSKSLITHPATTTHKNLTEEARAELGISPGTVRLSAGIEDTDDLIEDFAKALAKVSA

Sequences:

>Translated_394_residues
MSKTWRPATQLVHGGTLRSQYGETSEAIYLTQGFVYETSEAAEARFKGETEGYIYARYGSPTNDMFEKRMCMLEGAEDAR
ATASGMAAVSAAILCQVKAGDHIVAARALFGSCRWVVETLAPKYGVECTLVDGRDLANWEKAIRPNTKVFFLESPTNPTL
EVVDISGVAKLANQIGAKVVVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLADKDWIDENLHDYFRHTGP
AMSPFNAWTLLKGIETLPLRVKQQTGNAAKIADFLAEQSKVAKVIYPGRKDHPQADIIAKQMTGGSTLVAFELKGGKEAA
FALQNALDIVKISNNLGDSKSLITHPATTTHKNLTEEARAELGISPGTVRLSAGIEDTDDLIEDFAKALAKVSA
>Mature_393_residues
SKTWRPATQLVHGGTLRSQYGETSEAIYLTQGFVYETSEAAEARFKGETEGYIYARYGSPTNDMFEKRMCMLEGAEDARA
TASGMAAVSAAILCQVKAGDHIVAARALFGSCRWVVETLAPKYGVECTLVDGRDLANWEKAIRPNTKVFFLESPTNPTLE
VVDISGVAKLANQIGAKVVVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLADKDWIDENLHDYFRHTGPA
MSPFNAWTLLKGIETLPLRVKQQTGNAAKIADFLAEQSKVAKVIYPGRKDHPQADIIAKQMTGGSTLVAFELKGGKEAAF
ALQNALDIVKISNNLGDSKSLITHPATTTHKNLTEEARAELGISPGTVRLSAGIEDTDDLIEDFAKALAKVSA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=368, Percent_Identity=34.5108695652174, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI299473759, Length=368, Percent_Identity=31.25, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI25453487, Length=367, Percent_Identity=29.1553133514986, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1790375, Length=369, Percent_Identity=34.1463414634146, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=27.5510204081633, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=40.3614457831325, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17538055, Length=345, Percent_Identity=38.5507246376812, Blast_Score=240, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=34.2245989304813, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=36.5482233502538, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=31.3106796116505, Blast_Score=179, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=275, Percent_Identity=33.8181818181818, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321254, Length=409, Percent_Identity=27.1393643031785, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=26.6331658291457, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=36.046511627907, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42574; Mature: 42443

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTWRPATQLVHGGTLRSQYGETSEAIYLTQGFVYETSEAAEARFKGETEGYIYARYGS
CCCCCCCHHHHHCCCCHHHHCCCCCCEEEEEECEEEECCHHHHHHCCCCCCCEEEEEECC
PTNDMFEKRMCMLEGAEDARATASGMAAVSAAILCQVKAGDHIVAARALFGSCRWVVETL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEHEEEECCCCEEEEHHHHHHHHHHHHHHH
APKYGVECTLVDGRDLANWEKAIRPNTKVFFLESPTNPTLEVVDISGVAKLANQIGAKVV
CCCCCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEECHHHHHHHHHCCCEEE
VDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLADKDWIDENLHDYFRHTGP
EHHHHHCHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCC
AMSPFNAWTLLKGIETLPLRVKQQTGNAAKIADFLAEQSKVAKVIYPGRKDHPQADIIAK
CCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
QMTGGSTLVAFELKGGKEAAFALQNALDIVKISNNLGDSKSLITHPATTTHKNLTEEARA
HCCCCCEEEEEEECCCCHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCCHHHHHHHHHH
ELGISPGTVRLSAGIEDTDDLIEDFAKALAKVSA
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKTWRPATQLVHGGTLRSQYGETSEAIYLTQGFVYETSEAAEARFKGETEGYIYARYGS
CCCCCCHHHHHCCCCHHHHCCCCCCEEEEEECEEEECCHHHHHHCCCCCCCEEEEEECC
PTNDMFEKRMCMLEGAEDARATASGMAAVSAAILCQVKAGDHIVAARALFGSCRWVVETL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEHEEEECCCCEEEEHHHHHHHHHHHHHHH
APKYGVECTLVDGRDLANWEKAIRPNTKVFFLESPTNPTLEVVDISGVAKLANQIGAKVV
CCCCCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEECHHHHHHHHHCCCEEE
VDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLADKDWIDENLHDYFRHTGP
EHHHHHCHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCC
AMSPFNAWTLLKGIETLPLRVKQQTGNAAKIADFLAEQSKVAKVIYPGRKDHPQADIIAK
CCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
QMTGGSTLVAFELKGGKEAAFALQNALDIVKISNNLGDSKSLITHPATTTHKNLTEEARA
HCCCCCEEEEEEECCCCHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCCHHHHHHHHHH
ELGISPGTVRLSAGIEDTDDLIEDFAKALAKVSA
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]