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The map label for this gene is cspA [C]

Identifier: 209547690

GI number: 209547690

Start: 76098

End: 76307

Strand: Reverse

Name: cspA [C]

Synonym: Rleg2_0078

Alternate gene names: 209547690

Gene position: 76307-76098 (Counterclockwise)

Preceding gene: 209547691

Following gene: 209547689

Centisome position: 1.68

GC content: 56.19

Gene sequence:

>210_bases
ATGACCACTGGCACCGTTAAATGGTTCAACTCCACCAAGGGCTTCGGCTTCATTCAGCCTGACAACGGCGGCGCTGACGC
CTTCGTTCACATCTCCGCCGTCGAGCGCGCAGGAATGCGCGAACTCGTCGAAGGCCAGAAGATCGGTTTCGATCTCGAGC
GTGACAACAAGTCGGGCAAGATGTCCGCTTGCAATCTCCAAAACGCTTAA

Upstream 100 bases:

>100_bases
GGCACCGCGGCCTGAACGAACTTCCTTTCAAAAATCGCTATCACCATCCGTATCGCCCTTGGCGCTGCGGTTCTTCAATA
TTGCTATGAAAGGGTTCATC

Downstream 100 bases:

>100_bases
ATCGATATCTGCTTTCCTTTGACCACGGATGTACTGGCACTGCCGGAAGCACGTTATCCATTCAAGGCCAGGCGATCTGC
CTGGCCTTTTTGTTTTTGCT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MTTGTVKWFNSTKGFGFIQPDNGGADAFVHISAVERAGMRELVEGQKIGFDLERDNKSGKMSACNLQNA

Sequences:

>Translated_69_residues
MTTGTVKWFNSTKGFGFIQPDNGGADAFVHISAVERAGMRELVEGQKIGFDLERDNKSGKMSACNLQNA
>Mature_68_residues
TTGTVKWFNSTKGFGFIQPDNGGADAFVHISAVERAGMRELVEGQKIGFDLERDNKSGKMSACNLQNA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=55, Percent_Identity=56.3636363636364, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787839, Length=51, Percent_Identity=56.8627450980392, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI2367114, Length=52, Percent_Identity=53.8461538461538, Blast_Score=65, Evalue=7e-13,
Organism=Escherichia coli, GI1786841, Length=49, Percent_Identity=53.0612244897959, Blast_Score=65, Evalue=8e-13,
Organism=Escherichia coli, GI1787107, Length=62, Percent_Identity=48.3870967741936, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788126, Length=54, Percent_Identity=50, Blast_Score=63, Evalue=5e-12,
Organism=Escherichia coli, GI1787834, Length=55, Percent_Identity=49.0909090909091, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7480; Mature: 7349

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGTVKWFNSTKGFGFIQPDNGGADAFVHISAVERAGMRELVEGQKIGFDLERDNKSGK
CCCCEEEECCCCCCCEEECCCCCCCCEEEEEEHHHHCCHHHHHCCCCCCCEECCCCCCCC
MSACNLQNA
EEECCCCCC
>Mature Secondary Structure 
TTGTVKWFNSTKGFGFIQPDNGGADAFVHISAVERAGMRELVEGQKIGFDLERDNKSGK
CCCEEEECCCCCCCEEECCCCCCCCEEEEEEHHHHCCHHHHHCCCCCCCEECCCCCCCC
MSACNLQNA
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]