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The map label for this gene is gacS [H]

Identifier: 209547648

GI number: 209547648

Start: 37924

End: 40203

Strand: Reverse

Name: gacS [H]

Synonym: Rleg2_0036

Alternate gene names: 209547648

Gene position: 40203-37924 (Counterclockwise)

Preceding gene: 209547649

Following gene: 209547647

Centisome position: 0.89

GC content: 65.96

Gene sequence:

>2280_bases
ATGCACAATCTGGGACAGTTGCAGGAAAGATTGTCCGCCGCCTTCGGCGGACCCGCCGCCAAGCCGCGTGAAGAGCGGAT
GGCGGAGTTCTTCTCGCGACCCGGCACATCGGCGCCTCAAGCGGCCGACGACGCCAAGGTGGGGTCTGCAGCGACACGGC
GCCTGCTGGTCTATTGGCTTGGCGGCGCTGGCCTGCTTGCCGCATTGATCCTGACGCTGCTTGCTCATGCCGGCGATCCT
CTGCTGCTTTCGGGCGGTCTTGTCGTCCTCGGCCTTGCCGTCATATCAAGCTATGCCGTCCTGATGTTTCGCCGGCGCAG
GCCCCCTAGCGGCGGCAAATCCCTGCCGGATTGGAACGACGGCGCCAAGCTCTTCGCAGACGTCCATGACGTGCTCGGCG
ATATCACCGTCAGCCGCAGCATGGACCGGCGCATCATCTCCGCCAACGATACGTTTCGCCGCCTGACCGGGCGGCTGCGT
CCGGAAGGACACAGTTGCGAGGAGATCGGGCTTGCCTTTCGCCCCGGCCCGATACCGCACTGCTTCGATGTCGAGATCTC
GACACCGGACGGCCAGCGCATCTTTCTCTGGCGCGACGTCGTCACCCGCGACCCGGCAAACGGCCGGCTGCTGCTGCAGA
GTGTCGCGCGTGACGTGACCGACGAACGGTTGATCGCGCAGGGCCGCGAGGAGGCCCGCCAGAAGGCCGAATATAACAGC
GCCGCCAAATCGAGGCTGCTTGCCACCGTCAGCCACGAGGTGCGCACGCCGCTGTCCGGCCTGCTCGGCATGACGCATCT
GCTCGCCGAGACGCGGCTGACGCAGGAGCAGCAGGACTATCTCGCCAATATCCGCCAGTCCGGCCACGCGCTGACGCAGT
TGGTCGAGGATCTGCTCGATTTTTCCACCATCGAGGTCGGCCGCTTCGCGCTGCACCCGCGCTCGGAATCGCTGCGCAAG
CTTCTGGAAAGCGTCGTCGAGATGCTCGCCCACCGGGCGCATGAAAAAGGCATCGAGATCGGCGCCACGGTGTCCTCTGA
TGTGCCGGAGAATATGAGTTTCGACCCGGCGCGGCTGCGCCAGGTTCTGTTCAACGTCATCGGCAATGCGGTGAAGTTCA
CCCAGGTCGGCGGCGTCTTCATTCGCGTGTCGCTCGATGCCGACGACCTGTCGATCACCGTCACCGATAGCGGCCCCGGC
ATGACGGCGGAGGAGCAGGCGCGCGTCTTCGGCGAGTTCGAGCAGGGCGGGACCGTTGCCGACAGGAGCAGCGGCACCGG
GCTCGGCCTTGCCATCTCTGCCCGCATCATGCGCGAATTCAACGGCACGCTGACGGTTGCCAGCGAAAAGGGCAGAGGCA
GCGAATTCACCATCCGCTTCCCTGTCGATATCGGCAGCGAGCGCCCCGACCGGCGCAACACACTGCTTGCCGGCAACAGC
GTCGTGCTCCTGGCGCCGACAGGTGCGGCGCGCACCGCCATCGCCGAGACGATCACCGCGCTCGGCGGCCTCTGCCATCT
CGTCGCCGACGGCGAGAGCGCGCGGGCGACGTTGCTCGGCTTGGCGAATAGCGGAAAGCGGCCGACCGATATCATCATCG
ACCATCGCATGTCGGCGGAATTTGCCGCCCATCTTGCCGATCGCGCCGAAATCGCCGCCCTTGGGCTGCGCAAGGTCCTG
CTCGTCAATCCGGAAGAGCGCAGCGCCCATCCGCTCGACCTCTTCGATGCCTGGCTCATCCGGCCGCTGCGCGAACAGTC
GCTGATCGACGTGCTGCGCGGGCGCATGCGCGGCATGGAAAAGCGCGACGCGCTGAACGACAACCAGCCGGGCTTCGGCC
TTTCTGTGGCGGAGCCGATGGTTGCCGCCAGCGGGCTCAACATCCTGCTTGGCGAGGACGATCCGATCAACGCCATGCTG
GTGCGCGTCGTGCTGGAGAAGGGCGGCCATAAGGTGCGCCATGTCGAGGATTTCGAGGCGCTGCTCGATTGCGCCCTCTG
CGAGGCGAATGCCCGGCCCGATATCATCATCAGCGACCTGTCGATGCCCGGCGGCAACGGCATCGACATGCTCGGACGCC
TGCGCGGCCACGAAAGACGGCTCGATCTGGCTTCGGTACCGGTGATTGTGCTGACCGCCGACAAGAGCGACGAATCGCGC
CGCCAGGTGTTGCTGAACGGCGCCAACCGGGTGATGGTGAAGCCAGTCGACCCGGTGCGGCTACTGACCGAAGTTCAGGC
GGTCGCCGCACTTTCCGCCCGCCGCGCAGAGGCGCGGTAA

Upstream 100 bases:

>100_bases
GTCATTCTCGACGAGCTGCTGCTGCCGAAAAAGGCCGGCCGCTAAAATCTTATCGAATTCCTGAAGGCATTCGGAAGAGG
CCTCATGTTAGCCAGATTGC

Downstream 100 bases:

>100_bases
CGTGCCGCGGGGCCGGCATGCTTCTTGCGTTCACAAATTCGATATGTCACTTTCCTGTCGCAGAGAAGTGTTTTATGGCG
TCGATAAGCCGGCAACGGGA

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MHNLGQLQERLSAAFGGPAAKPREERMAEFFSRPGTSAPQAADDAKVGSAATRRLLVYWLGGAGLLAALILTLLAHAGDP
LLLSGGLVVLGLAVISSYAVLMFRRRRPPSGGKSLPDWNDGAKLFADVHDVLGDITVSRSMDRRIISANDTFRRLTGRLR
PEGHSCEEIGLAFRPGPIPHCFDVEISTPDGQRIFLWRDVVTRDPANGRLLLQSVARDVTDERLIAQGREEARQKAEYNS
AAKSRLLATVSHEVRTPLSGLLGMTHLLAETRLTQEQQDYLANIRQSGHALTQLVEDLLDFSTIEVGRFALHPRSESLRK
LLESVVEMLAHRAHEKGIEIGATVSSDVPENMSFDPARLRQVLFNVIGNAVKFTQVGGVFIRVSLDADDLSITVTDSGPG
MTAEEQARVFGEFEQGGTVADRSSGTGLGLAISARIMREFNGTLTVASEKGRGSEFTIRFPVDIGSERPDRRNTLLAGNS
VVLLAPTGAARTAIAETITALGGLCHLVADGESARATLLGLANSGKRPTDIIIDHRMSAEFAAHLADRAEIAALGLRKVL
LVNPEERSAHPLDLFDAWLIRPLREQSLIDVLRGRMRGMEKRDALNDNQPGFGLSVAEPMVAASGLNILLGEDDPINAML
VRVVLEKGGHKVRHVEDFEALLDCALCEANARPDIIISDLSMPGGNGIDMLGRLRGHERRLDLASVPVIVLTADKSDESR
RQVLLNGANRVMVKPVDPVRLLTEVQAVAALSARRAEAR

Sequences:

>Translated_759_residues
MHNLGQLQERLSAAFGGPAAKPREERMAEFFSRPGTSAPQAADDAKVGSAATRRLLVYWLGGAGLLAALILTLLAHAGDP
LLLSGGLVVLGLAVISSYAVLMFRRRRPPSGGKSLPDWNDGAKLFADVHDVLGDITVSRSMDRRIISANDTFRRLTGRLR
PEGHSCEEIGLAFRPGPIPHCFDVEISTPDGQRIFLWRDVVTRDPANGRLLLQSVARDVTDERLIAQGREEARQKAEYNS
AAKSRLLATVSHEVRTPLSGLLGMTHLLAETRLTQEQQDYLANIRQSGHALTQLVEDLLDFSTIEVGRFALHPRSESLRK
LLESVVEMLAHRAHEKGIEIGATVSSDVPENMSFDPARLRQVLFNVIGNAVKFTQVGGVFIRVSLDADDLSITVTDSGPG
MTAEEQARVFGEFEQGGTVADRSSGTGLGLAISARIMREFNGTLTVASEKGRGSEFTIRFPVDIGSERPDRRNTLLAGNS
VVLLAPTGAARTAIAETITALGGLCHLVADGESARATLLGLANSGKRPTDIIIDHRMSAEFAAHLADRAEIAALGLRKVL
LVNPEERSAHPLDLFDAWLIRPLREQSLIDVLRGRMRGMEKRDALNDNQPGFGLSVAEPMVAASGLNILLGEDDPINAML
VRVVLEKGGHKVRHVEDFEALLDCALCEANARPDIIISDLSMPGGNGIDMLGRLRGHERRLDLASVPVIVLTADKSDESR
RQVLLNGANRVMVKPVDPVRLLTEVQAVAALSARRAEAR
>Mature_759_residues
MHNLGQLQERLSAAFGGPAAKPREERMAEFFSRPGTSAPQAADDAKVGSAATRRLLVYWLGGAGLLAALILTLLAHAGDP
LLLSGGLVVLGLAVISSYAVLMFRRRRPPSGGKSLPDWNDGAKLFADVHDVLGDITVSRSMDRRIISANDTFRRLTGRLR
PEGHSCEEIGLAFRPGPIPHCFDVEISTPDGQRIFLWRDVVTRDPANGRLLLQSVARDVTDERLIAQGREEARQKAEYNS
AAKSRLLATVSHEVRTPLSGLLGMTHLLAETRLTQEQQDYLANIRQSGHALTQLVEDLLDFSTIEVGRFALHPRSESLRK
LLESVVEMLAHRAHEKGIEIGATVSSDVPENMSFDPARLRQVLFNVIGNAVKFTQVGGVFIRVSLDADDLSITVTDSGPG
MTAEEQARVFGEFEQGGTVADRSSGTGLGLAISARIMREFNGTLTVASEKGRGSEFTIRFPVDIGSERPDRRNTLLAGNS
VVLLAPTGAARTAIAETITALGGLCHLVADGESARATLLGLANSGKRPTDIIIDHRMSAEFAAHLADRAEIAALGLRKVL
LVNPEERSAHPLDLFDAWLIRPLREQSLIDVLRGRMRGMEKRDALNDNQPGFGLSVAEPMVAASGLNILLGEDDPINAML
VRVVLEKGGHKVRHVEDFEALLDCALCEANARPDIIISDLSMPGGNGIDMLGRLRGHERRLDLASVPVIVLTADKSDESR
RQVLLNGANRVMVKPVDPVRLLTEVQAVAALSARRAEAR

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=533, Percent_Identity=30.0187617260788, Blast_Score=195, Evalue=9e-51,
Organism=Escherichia coli, GI145693157, Length=526, Percent_Identity=28.8973384030418, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI48994928, Length=347, Percent_Identity=31.700288184438, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87081816, Length=260, Percent_Identity=36.1538461538462, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788713, Length=271, Percent_Identity=33.210332103321, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=31.1688311688312, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=29.2490118577075, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=28.5123966942149, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI87082128, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=28.6995515695067, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1788393, Length=246, Percent_Identity=27.2357723577236, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=25.6756756756757, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786600, Length=269, Percent_Identity=27.5092936802974, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789808, Length=225, Percent_Identity=28, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790300, Length=268, Percent_Identity=26.865671641791, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=361, Percent_Identity=23.8227146814404, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1788279, Length=214, Percent_Identity=25.7009345794392, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 82200; Mature: 82200

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLGQLQERLSAAFGGPAAKPREERMAEFFSRPGTSAPQAADDAKVGSAATRRLLVYWL
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEE
GGAGLLAALILTLLAHAGDPLLLSGGLVVLGLAVISSYAVLMFRRRRPPSGGKSLPDWND
CCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GAKLFADVHDVLGDITVSRSMDRRIISANDTFRRLTGRLRPEGHSCEEIGLAFRPGPIPH
HHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCCHHHCCCEECCCCCCC
CFDVEISTPDGQRIFLWRDVVTRDPANGRLLLQSVARDVTDERLIAQGREEARQKAEYNS
EEEEEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKSRLLATVSHEVRTPLSGLLGMTHLLAETRLTQEQQDYLANIRQSGHALTQLVEDLLD
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
FSTIEVGRFALHPRSESLRKLLESVVEMLAHRAHEKGIEIGATVSSDVPENMSFDPARLR
CCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEECCEECCCCCCCCCCCHHHHH
QVLFNVIGNAVKFTQVGGVFIRVSLDADDLSITVTDSGPGMTAEEQARVFGEFEQGGTVA
HHHHHHHCCCEEEEEECCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
DRSSGTGLGLAISARIMREFNGTLTVASEKGRGSEFTIRFPVDIGSERPDRRNTLLAGNS
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEECCE
VVLLAPTGAARTAIAETITALGGLCHLVADGESARATLLGLANSGKRPTDIIIDHRMSAE
EEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCEEEEECCHHHH
FAAHLADRAEIAALGLRKVLLVNPEERSAHPLDLFDAWLIRPLREQSLIDVLRGRMRGME
HHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KRDALNDNQPGFGLSVAEPMVAASGLNILLGEDDPINAMLVRVVLEKGGHKVRHVEDFEA
HHHCCCCCCCCCCCCHHCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
LLDCALCEANARPDIIISDLSMPGGNGIDMLGRLRGHERRLDLASVPVIVLTADKSDESR
HHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHCCCCCCCCEEEEECCCCCHHH
RQVLLNGANRVMVKPVDPVRLLTEVQAVAALSARRAEAR
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHNLGQLQERLSAAFGGPAAKPREERMAEFFSRPGTSAPQAADDAKVGSAATRRLLVYWL
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEE
GGAGLLAALILTLLAHAGDPLLLSGGLVVLGLAVISSYAVLMFRRRRPPSGGKSLPDWND
CCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GAKLFADVHDVLGDITVSRSMDRRIISANDTFRRLTGRLRPEGHSCEEIGLAFRPGPIPH
HHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCCHHHCCCEECCCCCCC
CFDVEISTPDGQRIFLWRDVVTRDPANGRLLLQSVARDVTDERLIAQGREEARQKAEYNS
EEEEEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKSRLLATVSHEVRTPLSGLLGMTHLLAETRLTQEQQDYLANIRQSGHALTQLVEDLLD
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
FSTIEVGRFALHPRSESLRKLLESVVEMLAHRAHEKGIEIGATVSSDVPENMSFDPARLR
CCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEECCEECCCCCCCCCCCHHHHH
QVLFNVIGNAVKFTQVGGVFIRVSLDADDLSITVTDSGPGMTAEEQARVFGEFEQGGTVA
HHHHHHHCCCEEEEEECCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
DRSSGTGLGLAISARIMREFNGTLTVASEKGRGSEFTIRFPVDIGSERPDRRNTLLAGNS
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEECCE
VVLLAPTGAARTAIAETITALGGLCHLVADGESARATLLGLANSGKRPTDIIIDHRMSAE
EEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCEEEEECCHHHH
FAAHLADRAEIAALGLRKVLLVNPEERSAHPLDLFDAWLIRPLREQSLIDVLRGRMRGME
HHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KRDALNDNQPGFGLSVAEPMVAASGLNILLGEDDPINAMLVRVVLEKGGHKVRHVEDFEA
HHHCCCCCCCCCCCCHHCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
LLDCALCEANARPDIIISDLSMPGGNGIDMLGRLRGHERRLDLASVPVIVLTADKSDESR
HHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHCCCCCCCCEEEEECCCCCHHH
RQVLLNGANRVMVKPVDPVRLLTEVQAVAALSARRAEAR
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]