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The map label for this gene is miaB [H]

Identifier: 209547631

GI number: 209547631

Start: 17882

End: 19291

Strand: Reverse

Name: miaB [H]

Synonym: Rleg2_0019

Alternate gene names: 209547631

Gene position: 19291-17882 (Counterclockwise)

Preceding gene: 209547632

Following gene: 209547630

Centisome position: 0.43

GC content: 62.77

Gene sequence:

>1410_bases
ATGACACAGGACAACGCCCTCCTTCAGGCCCCGGAGCTGACGCTCCGCGATGGTTCCAACAGCCGCAAGGTCTTCATCAA
GACCTATGGCTGCCAGATGAACGTCTATGATTCCACGCGCATGAGCGATGCGCTCGCCCGCGATGGCTATGAACCGACCG
AAGATATGGAAGAGGCGGACCTCGTTCTGCTCAACACCTGCCATATACGAGAGAAGGCGGCCGAGAAGGTCTATTCGGCG
CTCGGACGGCTGCGCGACATGAAAAAGAAGAAGGCGGCGGACGGACGGGAGATGATGATCGGCGTTGCCGGCTGCGTCGC
CCAGGCCGAAGGCGAGGAGATCCTGCGCCGGGCGCCCGCCGTCGACGTCGTCATCGGCCCGCAGACCTATCACCGCCTGC
CGGAGGCGCTGCGCCGGGCCAAGGAGGGCCAGCGCGTCGTCGATACGGACTATGCGATCGAGGACAAGTTCGAGCATCTG
CCGATTGCCGAGAGCCGGAAGATCCGCGCCCGCGGCGTCACCGCCTTCCTGACGGTGCAGGAGGGCTGCGACAAGTTCTG
CACCTTCTGCGTTGTGCCCTATACGCGCGGCTCGGAAGTGTCGCGGCCGGTTGCCCAGATCGTCGAGGAAGCCGAAAAGC
TCGTCGAGGGGGGCGTGCGCGAGATCACCCTGCTCGGGCAGAACGTCAATGCCTGGCACGGCGCCGGGCCGCGGGGCGAG
GCATGGAGCCTCGGCGACCTGCTCTACCGGCTTGCCGAGATCCCCGGCCTTGCGCGGCTGCGCTACACCACCAGCCATCC
GCGCGACATGGACGACCGGCTGATCGACGCCCATCGCGATCTCCGGGCGCTGATGCCCTATCTGCACTTGCCGGTGCAAT
CGGGCTCCGACCGCATCCTCAAAGCCATGAACCGGCGCCACACGGCAGCCGAATATCTCAGCCTGATTGCGCGCATCCGC
ACGGTGCGCCCCGATATCGCGCTGTCGGGCGATTTCATTACCGGCTTTCCGGGGGAGACAGACGCCGATTTTGAGGATAC
ACTCAGGCTTGTGGAGGAGGTCCGTTATGCACAGGCCTTTTCGTTCAAATACTCGACACGGCCGGGCACGCCCGGCGCGG
AACTGAAGGACCAGGTGCCGGAACAGATCAAGGCAGAACGGCTGGAACGCCTGCAAGCGCTTCTCCTGAAGCAGCAGCAG
GAATTTGCCGAATCCTGCATCGGCAAAGAGATCGACCTGTTGCTGGAAAAGCCCGGTCGCATGCCGGAACAGCTGATCGG
CCGTTCTCCCTGGCTTCAATCCGTGAATGTTGATGCAAAAGCATCGCAAATCGGTGACATTATTAAAGTGCGAATCACCG
GAACCGGAAACAACAGCCTGTTTGCCGAACGCGCAGAGGCTGCGGTTTAA

Upstream 100 bases:

>100_bases
CGCATCCGCAACCTGTTGAACAGCCGCCTGCGTGGGCGGGAAATCTCCTGAAAGAAGATTTCGATTTTCTCAGGCGGCAA
AAACCACTAAATAGCGCGCC

Downstream 100 bases:

>100_bases
CCCAAGGAGCCCGACCGCTTGAACGGACAAGAATTGGTTTCTTCTTCACCGCGCCACCCCCGCACGCCGAGCGACTCCAA
TCACTTCGTCCTGACGTTCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTQDNALLQAPELTLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEADLVLLNTCHIREKAAEKVYSA
LGRLRDMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAKEGQRVVDTDYAIEDKFEHL
PIAESRKIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGE
AWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIDAHRDLRALMPYLHLPVQSGSDRILKAMNRRHTAAEYLSLIARIR
TVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQVPEQIKAERLERLQALLLKQQQ
EFAESCIGKEIDLLLEKPGRMPEQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGNNSLFAERAEAAV

Sequences:

>Translated_469_residues
MTQDNALLQAPELTLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEADLVLLNTCHIREKAAEKVYSA
LGRLRDMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAKEGQRVVDTDYAIEDKFEHL
PIAESRKIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGE
AWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIDAHRDLRALMPYLHLPVQSGSDRILKAMNRRHTAAEYLSLIARIR
TVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQVPEQIKAERLERLQALLLKQQQ
EFAESCIGKEIDLLLEKPGRMPEQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGNNSLFAERAEAAV
>Mature_468_residues
TQDNALLQAPELTLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEADLVLLNTCHIREKAAEKVYSAL
GRLRDMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAKEGQRVVDTDYAIEDKFEHLP
IAESRKIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGEA
WSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIDAHRDLRALMPYLHLPVQSGSDRILKAMNRRHTAAEYLSLIARIRT
VRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQVPEQIKAERLERLQALLLKQQQE
FAESCIGKEIDLLLEKPGRMPEQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGNNSLFAERAEAAV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=35.3684210526316, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=35.3684210526316, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI93277076, Length=448, Percent_Identity=28.7946428571429, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=48.1981981981982, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787057, Length=460, Percent_Identity=30.2173913043478, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=423, Percent_Identity=34.7517730496454, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=368, Percent_Identity=26.6304347826087, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=33.2635983263598, Blast_Score=274, Evalue=8e-74,
Organism=Drosophila melanogaster, GI19922432, Length=453, Percent_Identity=28.476821192053, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52423; Mature: 52292

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQDNALLQAPELTLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEAD
CCCCCCEEECCCEEEECCCCCCEEEEEEECCEEEECCCHHHHHHHHHCCCCCCCCHHHCC
LVLLNTCHIREKAAEKVYSALGRLRDMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCC
VDVVIGPQTYHRLPEALRRAKEGQRVVDTDYAIEDKFEHLPIAESRKIRARGVTAFLTVQ
CEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCHHHCCCEEEEEHH
EGCDKFCTFCVVPYTRGSEVSRPVAQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGE
HHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
AWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIDAHRDLRALMPYLHLPVQSGSDRIL
CCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KAMNRRHTAAEYLSLIARIRTVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
SFKYSTRPGTPGAELKDQVPEQIKAERLERLQALLLKQQQEFAESCIGKEIDLLLEKPGR
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
MPEQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGNNSLFAERAEAAV
CHHHHCCCCCCCCCCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure 
TQDNALLQAPELTLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEAD
CCCCCEEECCCEEEECCCCCCEEEEEEECCEEEECCCHHHHHHHHHCCCCCCCCHHHCC
LVLLNTCHIREKAAEKVYSALGRLRDMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCC
VDVVIGPQTYHRLPEALRRAKEGQRVVDTDYAIEDKFEHLPIAESRKIRARGVTAFLTVQ
CEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCHHHCCCEEEEEHH
EGCDKFCTFCVVPYTRGSEVSRPVAQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGE
HHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
AWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIDAHRDLRALMPYLHLPVQSGSDRIL
CCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KAMNRRHTAAEYLSLIARIRTVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
SFKYSTRPGTPGAELKDQVPEQIKAERLERLQALLLKQQQEFAESCIGKEIDLLLEKPGR
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
MPEQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGNNSLFAERAEAAV
CHHHHCCCCCCCCCCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA