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The map label for this gene is trmB [H]

Identifier: 209547624

GI number: 209547624

Start: 10780

End: 11481

Strand: Reverse

Name: trmB [H]

Synonym: Rleg2_0012

Alternate gene names: 209547624

Gene position: 11481-10780 (Counterclockwise)

Preceding gene: 209547625

Following gene: 209547621

Centisome position: 0.25

GC content: 62.39

Gene sequence:

>702_bases
ATGACGGATATGGAACGCCGCGGCCGGGCGACTGAAGCCTTCTTCGGTCGCCGCAAGGGCAAGGCCCTGCGCGAACACCA
GGCCGAGACGCTGAACAGCCTGCTGCCGGCATTCCTTATCGATCTGTCAGCGGCTCCACCGGAGCCGCTGACATCCCTCT
TCCCGGCTCCGGTCGAAAAATTGCGGCTGGAAATCGGCTTCGGCGGCGGCGAACATCTGATTCACCGCGCCCTGGAGAGG
CCTTCGACCGGCTTTATCGGCGTTGAACCCTTCGTCAATTCCATGCAGAAGCTGCTGTCGCGCATCGGTGAGACCGGGGC
GCGCAATATCCGCGTCTATAATGACGATGCGACGCAACTGCTCGACTGGCTGCCGGACGCTTCGCTCGATCAGATCGACC
TGCTCTATCCCGATCCCTGGCCGAAGCGGAAGCACTGGAAACGGCGCTTCGTCTCGAAGACCAATCTCGACCGCTTTCAC
CGCGTGCTGAAGCCCGGCGCCCTGTTCTGCTTCGCCTCCGACATCGATACCTATGTCAACTGGACGCTGCTCAAATGCCG
CGACCATGGCGGCTTCGACTGGCTGGCCGACAATGCCGCGGACTGGCTGACGCCCTATGAGGGCTGGCCCAGCACACGTT
ACGAGGCCAAGGCGCGGCGAGAGGGCCGGTCTTCGGCCTATCTGACGTTTAGGAAGATCTGA

Upstream 100 bases:

>100_bases
GCCATTTCGGCCGCAAGGCCGGTCGCGACGGCTCGTTCTCCTGGGAGCGCACGGACCTCGTCAAGGCGCTCAAGGAAAGC
GTGAAGGCCTGAGATAAAGC

Downstream 100 bases:

>100_bases
TTTTCAGTGAAGTCAGCGAACACGACGCAATGTTCGTCGGCGTGAGCGGATCAGGAAGCGGTTGAAGGGCGATGGTGAGC
GGCGTGCCGTGTAGCCCCCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTDMERRGRATEAFFGRRKGKALREHQAETLNSLLPAFLIDLSAAPPEPLTSLFPAPVEKLRLEIGFGGGEHLIHRALER
PSTGFIGVEPFVNSMQKLLSRIGETGARNIRVYNDDATQLLDWLPDASLDQIDLLYPDPWPKRKHWKRRFVSKTNLDRFH
RVLKPGALFCFASDIDTYVNWTLLKCRDHGGFDWLADNAADWLTPYEGWPSTRYEAKARREGRSSAYLTFRKI

Sequences:

>Translated_233_residues
MTDMERRGRATEAFFGRRKGKALREHQAETLNSLLPAFLIDLSAAPPEPLTSLFPAPVEKLRLEIGFGGGEHLIHRALER
PSTGFIGVEPFVNSMQKLLSRIGETGARNIRVYNDDATQLLDWLPDASLDQIDLLYPDPWPKRKHWKRRFVSKTNLDRFH
RVLKPGALFCFASDIDTYVNWTLLKCRDHGGFDWLADNAADWLTPYEGWPSTRYEAKARREGRSSAYLTFRKI
>Mature_232_residues
TDMERRGRATEAFFGRRKGKALREHQAETLNSLLPAFLIDLSAAPPEPLTSLFPAPVEKLRLEIGFGGGEHLIHRALERP
STGFIGVEPFVNSMQKLLSRIGETGARNIRVYNDDATQLLDWLPDASLDQIDLLYPDPWPKRKHWKRRFVSKTNLDRFHR
VLKPGALFCFASDIDTYVNWTLLKCRDHGGFDWLADNAADWLTPYEGWPSTRYEAKARREGRSSAYLTFRKI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=222, Percent_Identity=31.0810810810811, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26759; Mature: 26628

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMERRGRATEAFFGRRKGKALREHQAETLNSLLPAFLIDLSAAPPEPLTSLFPAPVEK
CCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHH
LRLEIGFGGGEHLIHRALERPSTGFIGVEPFVNSMQKLLSRIGETGARNIRVYNDDATQL
EEEEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
LDWLPDASLDQIDLLYPDPWPKRKHWKRRFVSKTNLDRFHRVLKPGALFCFASDIDTYVN
HHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHHHC
WTLLKCRDHGGFDWLADNAADWLTPYEGWPSTRYEAKARREGRSSAYLTFRKI
EEEEEEECCCCCCHHHCCCHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
TDMERRGRATEAFFGRRKGKALREHQAETLNSLLPAFLIDLSAAPPEPLTSLFPAPVEK
CCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHH
LRLEIGFGGGEHLIHRALERPSTGFIGVEPFVNSMQKLLSRIGETGARNIRVYNDDATQL
EEEEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
LDWLPDASLDQIDLLYPDPWPKRKHWKRRFVSKTNLDRFHRVLKPGALFCFASDIDTYVN
HHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHHHC
WTLLKCRDHGGFDWLADNAADWLTPYEGWPSTRYEAKARREGRSSAYLTFRKI
EEEEEEECCCCCCHHHCCCHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA