Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 209547621

GI number: 209547621

Start: 8472

End: 9437

Strand: Reverse

Name: rihA [H]

Synonym: Rleg2_0009

Alternate gene names: 209547621

Gene position: 9437-8472 (Counterclockwise)

Preceding gene: 209547624

Following gene: 209547618

Centisome position: 0.21

GC content: 62.63

Gene sequence:

>966_bases
ATGAAGAAAACGGTGCATCGGATGGCAAGCGCAAGAAAGATCATCATCGACACGGATCCCGGCCAGGACGACGCGGCCGC
CATCATGCTGGCCTTCGGCAGTCCCGACGAGCTGGAGCTGCTGGGGATCACCACGGTCGCCGGCAACGTGCCGCTTTCGC
TGACCAGCCGCAATGCGCGCATCGTCTGCGAACTCTGCGGCCGGACGGAGACGAAGGTCTTCGCCGGCGCCGACGCGCCG
ATCGCCCGCAAGCTGGTGACCGCCGAACATGTGCACGGCAAGACAGGCCTCGACGGTCCCGAACTCAGCGAGCCGACGAT
GGCGCTGCAGACCGGCCATGCCGTCGACTTCATCATCGAGACGCTGCGGCGTGAGGAAGAAGGCACGGTGACGCTCTGCA
CGCTCGGGCCGCTCACCAATATCGGCATGGCCTTGCAGAAGGCGCCCGACATCGCCCCGCGCATCCGCGAATTGGTGATG
ATGGGCGGCGGCTTTTTCGAGGGTGGCAACATCACCCCGGCGGCCGAATTCAACATCTATGTCGACCCCGAGGCAGCCGA
TATCGTCTTCCGCTCCGGCATACCGATCGTGATGATGCCGCTCGACGTGACGCACCAATTGCTGACCCGCAAGGATCGGG
TGAAGCGCATGGCCGAGATCGGCACGGCGCCTGTTAAGGCGATGGTCGAGATGCTGGAATTCTTCGAACGCTTCGATATC
GAGAAATACGGCTCCGACGGCGGGCCGCTGCATGACCCCACCGTGATCGCCTACCTGCTGAAGCCGGAGCTTTTCAACGG
CCGGGACTGCAATGTCGAGATCGAGATCCACTCCGAACTCACCGTCGGTATGACGGTCGTCGACTGGTGGCATGTCACCG
AGCGCAAGCGCAATGCAAAAGTGATGCGCCAAGTCGATGCGGACGGCTTCTTCGATCTGTTGATCGAACGCTTCGCCCGC
ATCTGA

Upstream 100 bases:

>100_bases
GCCCTCAATTTTAAGGTGGTGACGGCTCCTTGCTTGACAAAGCGGCGGCTGCCTCGCATCAAAACGTGCCGGCGCGGAAT
GTGCCGATTTGTGACGAGAA

Downstream 100 bases:

>100_bases
TTTCGGCACCCTTCTCCCAAGGGAGAAGGTTTCAGGCGTCCTTCTCGTCGAAGACCGAATTGGTTTCGGCTTCGGCGGCC
TTCGGGCCGCCCTTGGCGAC

Product: inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKKTVHRMASARKIIIDTDPGQDDAAAIMLAFGSPDELELLGITTVAGNVPLSLTSRNARIVCELCGRTETKVFAGADAP
IARKLVTAEHVHGKTGLDGPELSEPTMALQTGHAVDFIIETLRREEEGTVTLCTLGPLTNIGMALQKAPDIAPRIRELVM
MGGGFFEGGNITPAAEFNIYVDPEAADIVFRSGIPIVMMPLDVTHQLLTRKDRVKRMAEIGTAPVKAMVEMLEFFERFDI
EKYGSDGGPLHDPTVIAYLLKPELFNGRDCNVEIEIHSELTVGMTVVDWWHVTERKRNAKVMRQVDADGFFDLLIERFAR
I

Sequences:

>Translated_321_residues
MKKTVHRMASARKIIIDTDPGQDDAAAIMLAFGSPDELELLGITTVAGNVPLSLTSRNARIVCELCGRTETKVFAGADAP
IARKLVTAEHVHGKTGLDGPELSEPTMALQTGHAVDFIIETLRREEEGTVTLCTLGPLTNIGMALQKAPDIAPRIRELVM
MGGGFFEGGNITPAAEFNIYVDPEAADIVFRSGIPIVMMPLDVTHQLLTRKDRVKRMAEIGTAPVKAMVEMLEFFERFDI
EKYGSDGGPLHDPTVIAYLLKPELFNGRDCNVEIEIHSELTVGMTVVDWWHVTERKRNAKVMRQVDADGFFDLLIERFAR
I
>Mature_321_residues
MKKTVHRMASARKIIIDTDPGQDDAAAIMLAFGSPDELELLGITTVAGNVPLSLTSRNARIVCELCGRTETKVFAGADAP
IARKLVTAEHVHGKTGLDGPELSEPTMALQTGHAVDFIIETLRREEEGTVTLCTLGPLTNIGMALQKAPDIAPRIRELVM
MGGGFFEGGNITPAAEFNIYVDPEAADIVFRSGIPIVMMPLDVTHQLLTRKDRVKRMAEIGTAPVKAMVEMLEFFERFDI
EKYGSDGGPLHDPTVIAYLLKPELFNGRDCNVEIEIHSELTVGMTVVDWWHVTERKRNAKVMRQVDADGFFDLLIERFAR
I

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=47.8964401294498, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788486, Length=305, Percent_Identity=43.2786885245902, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786213, Length=308, Percent_Identity=39.9350649350649, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI32565474, Length=200, Percent_Identity=36, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17565698, Length=312, Percent_Identity=28.525641025641, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320608, Length=266, Percent_Identity=32.3308270676692, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24641837, Length=333, Percent_Identity=29.7297297297297, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24583915, Length=205, Percent_Identity=31.7073170731707, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24641839, Length=330, Percent_Identity=27.5757575757576, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 35370; Mature: 35370

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVHRMASARKIIIDTDPGQDDAAAIMLAFGSPDELELLGITTVAGNVPLSLTSRNAR
CCHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEECCCCE
IVCELCGRTETKVFAGADAPIARKLVTAEHVHGKTGLDGPELSEPTMALQTGHAVDFIIE
EEEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHH
TLRREEEGTVTLCTLGPLTNIGMALQKAPDIAPRIRELVMMGGGFFEGGNITPAAEFNIY
HHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEE
VDPEAADIVFRSGIPIVMMPLDVTHQLLTRKDRVKRMAEIGTAPVKAMVEMLEFFERFDI
ECCCHHEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
EKYGSDGGPLHDPTVIAYLLKPELFNGRDCNVEIEIHSELTVGMTVVDWWHVTERKRNAK
HHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCEEECEEEEEEHHHHHHHHHHH
VMRQVDADGFFDLLIERFARI
HHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTVHRMASARKIIIDTDPGQDDAAAIMLAFGSPDELELLGITTVAGNVPLSLTSRNAR
CCHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEECCCCE
IVCELCGRTETKVFAGADAPIARKLVTAEHVHGKTGLDGPELSEPTMALQTGHAVDFIIE
EEEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHH
TLRREEEGTVTLCTLGPLTNIGMALQKAPDIAPRIRELVMMGGGFFEGGNITPAAEFNIY
HHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEE
VDPEAADIVFRSGIPIVMMPLDVTHQLLTRKDRVKRMAEIGTAPVKAMVEMLEFFERFDI
ECCCHHEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
EKYGSDGGPLHDPTVIAYLLKPELFNGRDCNVEIEIHSELTVGMTVVDWWHVTERKRNAK
HHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCEEECEEEEEEHHHHHHHHHHH
VMRQVDADGFFDLLIERFARI
HHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA