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The map label for this gene is rph [H]

Identifier: 209547618

GI number: 209547618

Start: 5763

End: 6482

Strand: Reverse

Name: rph [H]

Synonym: Rleg2_0006

Alternate gene names: 209547618

Gene position: 6482-5763 (Counterclockwise)

Preceding gene: 209547621

Following gene: 209547617

Centisome position: 0.14

GC content: 63.61

Gene sequence:

>720_bases
ATGCGGCCTTCAGGCAGAAAAATCGACCAGATGCGCAAGGTCTCGTTCGAGCGCAATTTTTCCAAGCATGCGGAAGGCTC
CTGCCTGGTGAAATTCGGCGATACGCATGTGCTCTGCACGGCCAGCCTCGAAGAAAAGACGCCGCCGTGGCTGCGCAATT
CCGGCAAGGGCTGGGTCACGGCTGAGTACGGCATGCTGCCGCGGGCGACCGGCGAGCGCATGAAGCGCGAGGCCGCGGCC
GGCAAGCAGGGCGGCCGCACCCAGGAAATCCAGCGGCTGATCGGCCGGTCGCTGCGCGCCGTCGTCGATCTGCAGGCGCT
CGGCGAGCGGCAGATCACCCTTGATTGCGACGTCATCCAGGCCGATGGCGGCACCCGGACGGCCTCGATCACCGGTGGCT
GGATCGCGCTTTACGACTGCCTGAAATGGATGGAAAGCCGCAACATGATCAAGGTCGACCGGGTCCTGAAGGACCATGTC
GCCGCCATCTCCTGCGGCGTCTTCGCCAGCCAGCCGGTGATCGACCTCGATTACCTCGAGGATTCCTCGGCCGAAACCGA
CGCCAACTTCGTCATGACCGGCGCCGGCGGGATCGTCGAAATCCAGGGCACGGCTGAAGGCACGCCGTTTTCCGAAGAGG
AATTCACCTCGCTGATGGGTCTTGCCAAGAACGGCATCGGCGAACTCGTTGCCCTGCAGAAACAGGCGATTGCGGGATGA

Upstream 100 bases:

>100_bases
CTTCGCGAAAAGTAAGTCAAACTCGCAGCCGTGGCAAAAGGGAATTTGCAAATAGGCGCCGCCAATCGTAGAAGCGGTCA
ACAAACCCCAGGAGATAAGA

Downstream 100 bases:

>100_bases
ACCCCATGCTGGAAGGCATGTTGGAAACGGCGCTCTATGCGCGGGATCTCGATGAGGCCGAAGCGTTCTACGAGAACGTT
CTCGGATTTGAAAAGATCGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRPSGRKIDQMRKVSFERNFSKHAEGSCLVKFGDTHVLCTASLEEKTPPWLRNSGKGWVTAEYGMLPRATGERMKREAAA
GKQGGRTQEIQRLIGRSLRAVVDLQALGERQITLDCDVIQADGGTRTASITGGWIALYDCLKWMESRNMIKVDRVLKDHV
AAISCGVFASQPVIDLDYLEDSSAETDANFVMTGAGGIVEIQGTAEGTPFSEEEFTSLMGLAKNGIGELVALQKQAIAG

Sequences:

>Translated_239_residues
MRPSGRKIDQMRKVSFERNFSKHAEGSCLVKFGDTHVLCTASLEEKTPPWLRNSGKGWVTAEYGMLPRATGERMKREAAA
GKQGGRTQEIQRLIGRSLRAVVDLQALGERQITLDCDVIQADGGTRTASITGGWIALYDCLKWMESRNMIKVDRVLKDHV
AAISCGVFASQPVIDLDYLEDSSAETDANFVMTGAGGIVEIQGTAEGTPFSEEEFTSLMGLAKNGIGELVALQKQAIAG
>Mature_239_residues
MRPSGRKIDQMRKVSFERNFSKHAEGSCLVKFGDTHVLCTASLEEKTPPWLRNSGKGWVTAEYGMLPRATGERMKREAAA
GKQGGRTQEIQRLIGRSLRAVVDLQALGERQITLDCDVIQADGGTRTASITGGWIALYDCLKWMESRNMIKVDRVLKDHV
AAISCGVFASQPVIDLDYLEDSSAETDANFVMTGAGGIVEIQGTAEGTPFSEEEFTSLMGLAKNGIGELVALQKQAIAG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=212, Percent_Identity=61.7924528301887, Blast_Score=268, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71981632, Length=183, Percent_Identity=27.3224043715847, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25970; Mature: 25970

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRKIDQMRKVSFERNFSKHAEGSCLVKFGDTHVLCTASLEEKTPPWLRNSGKGWVT
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCHHHCCCCCEEE
AEYGMLPRATGERMKREAAAGKQGGRTQEIQRLIGRSLRAVVDLQALGERQITLDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGGWIALYDCLKWMESRNMIKVDRVLKDHVAAISCGVFASQPVIDLDYLE
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
DSSAETDANFVMTGAGGIVEIQGTAEGTPFSEEEFTSLMGLAKNGIGELVALQKQAIAG
CCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSGRKIDQMRKVSFERNFSKHAEGSCLVKFGDTHVLCTASLEEKTPPWLRNSGKGWVT
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCHHHCCCCCEEE
AEYGMLPRATGERMKREAAAGKQGGRTQEIQRLIGRSLRAVVDLQALGERQITLDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGGWIALYDCLKWMESRNMIKVDRVLKDHVAAISCGVFASQPVIDLDYLE
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
DSSAETDANFVMTGAGGIVEIQGTAEGTPFSEEEFTSLMGLAKNGIGELVALQKQAIAG
CCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA