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The map label for this gene is dnaQ [H]

Identifier: 209400894

GI number: 209400894

Start: 239600

End: 240331

Strand: Direct

Name: dnaQ [H]

Synonym: ECH74115_0227

Alternate gene names: 209400894

Gene position: 239600-240331 (Clockwise)

Preceding gene: 209400502

Following gene: 209399184

Centisome position: 4.3

GC content: 51.09

Gene sequence:

>732_bases
ATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGG
CCACAAGATCATTGAGATTGGTGCCGTTGAAGTGGTGAACCGTCGCCTGACGGGCAATAACTTCCATGTTTATCTCAAAC
CCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCC
GAAGTAGCCGATGAGTTCATGGACTATATCCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTAT
GGACTACGAGTTCTCGTTGCTTAAGCGCGATATTCCGAAGACTAATACCTTCTGTAAGGTCACCGATAGCCTTGCGGTGG
CGAGGAAAATGTTTCCCGGTAAACGCAACAGCCTCGATGCGTTATGTGCCCGCTACGAAATAGATAACAGTAAACGAACG
CTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTT
TGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATCCAGCGCCTTGTACGTCAGGCAAGTAAGTTACGCG
TTGTTTTTGCGACAGATGAAGAGCTTGCAGCACATGAAGCCCGTCTCGATCTGGTGCAGAAAAAGGGCGGAAGCTGCCTC
TGGCGGGCATAA

Upstream 100 bases:

>100_bases
CGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAA
GTCTGACATAAATGACCGCT

Downstream 100 bases:

>100_bases
ATACATGTAAAAGGCGCTAAAAATAGCGACTTGGGCGATTTTTGCAGCAAATGATTCAAAAGATGAGAAAAACCGTTGAC
GAAGGGTGAGGCAATCCGTA

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFA
EVADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFATDEELAAHEARLDLVQKKGGSCL
WRA

Sequences:

>Translated_243_residues
MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFA
EVADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFATDEELAAHEARLDLVQKKGGSCL
WRA
>Mature_242_residues
STAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAE
VADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTL
HGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFATDEELAAHEARLDLVQKKGGSCLW
RA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=243, Percent_Identity=99.1769547325103, Blast_Score=503, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 27099; Mature: 26968

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDP
CCCCCEEEEEEECCCCCCHHHCCCCCCCEEEEECHHHHHHHHCCCCCEEEEECCCCCCCC
EAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPK
CCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECEEECEEECHHHHHHHHCCCC
TNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTLHGALLDAQILAEVYLAMTG
CCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFATDEELAAHEARLDLVQKKGGSCL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCEE
WRA
CCC
>Mature Secondary Structure 
STAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDP
CCCCEEEEEEECCCCCCHHHCCCCCCCEEEEECHHHHHHHHCCCCCEEEEECCCCCCCC
EAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPK
CCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECEEECEEECHHHHHHHHCCCC
TNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTLHGALLDAQILAEVYLAMTG
CCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFATDEELAAHEARLDLVQKKGGSCL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCEE
WRA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3023634; 6316347; 3540531; 9278503; 1575709 [H]