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The map label for this gene is narY [H]

Identifier: 209400809

GI number: 209400809

Start: 1971145

End: 1972689

Strand: Reverse

Name: narY [H]

Synonym: ECH74115_2077

Alternate gene names: 209400809

Gene position: 1972689-1971145 (Counterclockwise)

Preceding gene: 209398888

Following gene: 209400611

Centisome position: 35.4

GC content: 54.17

Gene sequence:

>1545_bases
ATGAAAATCCGTTCGCAAGTCGGCATGGTGCTTAATCTCGATAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAA
AAACGTCTGGACCGGACGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGCATTGGTTATCCGA
AAAACTGGGAAGATCAGGAAGAGTGGCAAGGCGGCTGGGTCCGCGATGTGAATGGCAAGATACGCCCGCGTCTGGGCAGC
AAGATGGGTGTAATAACCAAAATCTTCGCCAACCCGGTGGTGCCGCAGATTGATGATTACTACGAACCTTTCACCTTCGA
CTACGAACATTTGCATAGCGCACCGGAAGGCAAACATATCCCTACTGCTCGCCCGCGTTCGCTGATTGACGGTAAGCGGA
TGGACAAAGTGATCTGGGGGCCAAACTGGGAAGAACTGCTGGGCGGCGAGTTTGAAAAACGTGCCCGCGACCGCAACTTC
GAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
CAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGG
ATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCA
GAAAAATGTATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCG
ATATCTGGGCGTGCTGCTTTACGACGCTGACCGCATCGAGGAAGCGGCGAGCACCGAGCGCGAGGTTGACCTCTATGAAC
GCCAGTGCGAAGTGTTCCTCGATCCGCACGATCCCTCAGTGATCGAGGAAGCCCTGAAACAAGGTATTCCACAAAATGTG
ATTGACGCTGCCCAGCGTTCGCCAGTCTACAAAATGGCGATGGACTGGAAACTGGCACTACCGCTGCACCCTGAATACCG
CACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTCCTACGCAGATGCGGGCGGTTTGCCGAAAAGCG
AAGGCGTGCTGCCCGCCATCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGT
CCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAGGCGTTACTGATAC
TCGTGCCATCGACGAAGTAGGCCTGAGCATCGCCCAGGTCGAAGAAATGTATCGCTATCTCGCCATTGCCAACTATGAAG
ATCGTTTTGTTATCCCGACCAGCCATCGGGAAATGGCGGGCGATGCCTTCGCAGAACGCAATGGCTGCGGTTTTACCTTT
GGTGACGGTTGTCACGGCTCGGACAGCAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATCAACATCACCGAAGT
GCGCGACAAAGCGGAGGGCGAATAA

Upstream 100 bases:

>100_bases
CCGTCGGCTCGAACCGCGACGAATTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCAGATCCTCAAAGTGATCGGCCTGTTGATGGAGTATCCGGACGAGATGTTGTGGGAATGCAAGGAGGACGCGCTGGCG
TTGATCCGCCGCGATGCGCC

Product: nitrate reductase 2, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=99.6108949416342, Blast_Score=1073, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=78.8537549407115, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=41.5841584158416, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=112, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58545; Mature: 58545

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSIAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSIAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]