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The map label for this gene is puuD

Identifier: 209400761

GI number: 209400761

Start: 1836080

End: 1836832

Strand: Direct

Name: puuD

Synonym: ECH74115_1940

Alternate gene names: 209400761

Gene position: 1836080-1836832 (Clockwise)

Preceding gene: 209395704

Following gene: 209400310

Centisome position: 32.95

GC content: 53.52

Gene sequence:

>753_bases
ATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGGCATGCGACCCAGACTCTGCAAGAAAAGTA
CCTGAATGCCATCATCCACGCAGGCGGCTTGCCAATTGCGCTGCCACATGCGCTGGCGGAACCGTCGTTACTTGAACAAC
TTTTGCCGAAACTCGATGGCATTTACCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGAT
GAGCCTGACGCCGATCCCGAGCGTGATCTTCTGAGCATGGCGTTAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTT
CGCCATCTGCCGGGGTTTACAGGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAAT
TGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGG
GGATTGCTGTCTGCATTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTGCATGGACAAGGGGCGAAGGTCGTTAG
CCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGTGTCATCAATCATCCTTTTGCGCTGG
GCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGT
CAGCACCATATCGCTGAAAAACAGCGACTCTGA

Upstream 100 bases:

>100_bases
CCCACTGCTAGATTGAAAAAATATTGAACATAAAGGTCATTTAAACCGCAGTAATGGCGATAATTTAGTCCACTTTGTGA
GATTGAGAATGGAAAATATA

Downstream 100 bases:

>100_bases
CCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAG
CAGGGGCTTTCACAACGTCG

Product: gamma-glutamyl-gamma-aminobutyrate hydrolase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGD
EPDADPERDLLSMALINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEG
GLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGFITAC
QHHIAEKQRL

Sequences:

>Translated_250_residues
MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGD
EPDADPERDLLSMALINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEG
GLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGFITAC
QHHIAEKQRL
>Mature_250_residues
MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGD
EPDADPERDLLSMALINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEG
GLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGFITAC
QHHIAEKQRL

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI87081871, Length=250, Percent_Identity=99.2, Blast_Score=503, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUUD_ECO57 (Q8X7G2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90863
- PIR:   F85755
- RefSeq:   NP_287890.1
- RefSeq:   NP_309902.2
- ProteinModelPortal:   Q8X7G2
- SMR:   Q8X7G2
- EnsemblBacteria:   EBESCT00000028612
- EnsemblBacteria:   EBESCT00000058477
- GeneID:   912701
- GeneID:   961343
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2490
- KEGG:   ecs:ECs1875
- GeneTree:   EBGT00050000011130
- HOGENOM:   HBG668169
- OMA:   PEEMHAN
- ProtClustDB:   PRK11366
- BioCyc:   ECOL83334:ECS1875-MONOMER
- InterPro:   IPR017926
- InterPro:   IPR011697

Pfam domain/function: PF07722 Peptidase_C26

EC number: =3.5.1.94

Molecular weight: Translated: 27598; Mature: 27598

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 114-114 ACT_SITE 222-222 ACT_SITE 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDG
CCCCCEEHEEHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHHHHHHHHCC
IYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINAALERRIPIFAICRGLQELVVAT
EECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSNFWVNSLHG
CCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC
QGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGFITAC
CCCEEECCCEEEECCCCCHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHH
QHHIAEKQRL
HHHHHHHCCC
>Mature Secondary Structure
MNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDG
CCCCCEEHEEHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHHHHHHHHCC
IYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINAALERRIPIFAICRGLQELVVAT
EECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSNFWVNSLHG
CCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC
QGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGFITAC
CCCEEECCCEEEECCCCCHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHH
QHHIAEKQRL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796