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The map label for this gene is fumB [H]

Identifier: 209400759

GI number: 209400759

Start: 5275330

End: 5276976

Strand: Reverse

Name: fumB [H]

Synonym: ECH74115_5636

Alternate gene names: 209400759

Gene position: 5276976-5275330 (Counterclockwise)

Preceding gene: 209398687

Following gene: 209400315

Centisome position: 94.7

GC content: 54.34

Gene sequence:

>1647_bases
ATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGA
TTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAG
CCTTCCACGACGCTTCTTTCATGCTCCGTCCGGCACACCAGAAACAGGTTGCGGCTATTCTTCACGATCCAGAAGCCAGC
GAAAACGACAAGTACGTGGCACTGCAATTCTTAAGAAACTCCGAAATCGCCGCCAAAGGCGTGCTGCCGACCTGCCAGGA
TACCGGCACCGCGATCATCGTCGGTAAAAAAGGCCAGCGCGTGTGGACCGGTGGCGGTGATGAAGAAGCGCTGTCGAAAG
GCGTCTATAACACCTATATCGAAGATAACCTGCGCTACTCGCAAAACGCGGCGCTGGACATGTACAAAGAGGTCAACACC
GGCACTAACCTGCCTGCGCAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAAATTCCTTTGCGTTGCGAAAGGCGG
CGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCCCTGCTGACTCCCGGCAAACTGAAAAACTTCCTCGTCG
AGAAAATGCGTACCCTCGGTACTGCAGCCTGCCCGCCGTACCATATCGCGTTTGTGATTGGCGGTACGTCTGTGGAAACC
AACCTGAAAACCGTTAAGTTAGCAAGCGCTCACTATTACGATGAACTGCCGACGGAAGGGAACGAACATGGTCAGGCGTT
CCGCGATGTCCAGCTGGAACAGGAACTGCTGGAAGAGGCCCAGAAACTCGGTCTTGGCGCGCAGTTTGGCGGTAAATACT
TCGCGCACGACATTCGCGTTATCCGTCTGCCACGTCACGGCGCATCCTGCCCGGTAGGCATGGGCGTCTCCTGCTCCGCT
GACCGTAACATTAAAGCGAAAATCAACCGCGAAGGTATCTGGATCGAAAAACTGGAACACCATCCAGGTCAGTACATTCC
ACAAGAACTGCGCCAGGCCGGTGAAGGCGAAGCGGTGAAGGTCGACCTTAACCGCCCGATGAAAGAGATCCTCGCCCAGC
TTTCGCAATATCCGGTTTCCACTCGTTTGTCGCTCACCGGCACCATTATCGTGGGCCGAGATATTGCACACGCCAAGCTG
AAAGAGCTGATTGACGCCGGTAAAGAACTTCCGCAGTACATCAAAGATCACCCGATCTACTACGCGGGTCCGGCGAAAAC
CCCTGCCGGTTATCCATCAGGCTCACTTGGCCCAACCACCGCAGGTCGTATGGACTCCTATGTGGATCTGCTGCAATCCC
ACGGCGGCAGCATGATCATGCTAGCGAAAGGTAACCGCAGTCAGCAGGTTACCGACGCGTGTCATAAACACGGCGGCTTC
TACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAATGCGTCGCTTATCCGGA
GCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACT
TCTTCCAGCAAATCATCAACAAACAGTGCGCGAACTGCACTAAGTAA

Upstream 100 bases:

>100_bases
CCGCGATGTACGGGTTCTTATAAATGCACTTTGCGTGCCGCCCGTTACTACGCGGCACGCCATTTTCGAATAACAAATAC
AGAGTTACAGGCTGGAAGCT

Downstream 100 bases:

>100_bases
CCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGTGATCCCCCTGGGCCACCTCTTTTGCGATTGTAATTTCACGCTT
GCTGGTGAATAGTCAGTATT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSVET
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK

Sequences:

>Translated_548_residues
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSVET
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK
>Mature_547_residues
SNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASE
NDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNTG
TNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSVETN
LKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINREGIWIEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLK
ELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=99.2700729927007, Blast_Score=1133, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=89.7810218978102, Blast_Score=1026, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.8342541436464, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60141; Mature: 60010

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF
CCCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHCCCHH
MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EECCCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSVET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEC
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRV
CCEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHHPGQYIPQELRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEHHHCCCCCCCHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIIN
EEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCTK
HHHCCCCC
>Mature Secondary Structure 
SNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF
CCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHCCCHH
MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EECCCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSVET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEC
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRV
CCEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHHPGQYIPQELRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEHHHCCCCCCCHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIIN
EEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCTK
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]