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The map label for this gene is manA [H]

Identifier: 209400753

GI number: 209400753

Start: 2194672

End: 2195847

Strand: Direct

Name: manA [H]

Synonym: ECH74115_2323

Alternate gene names: 209400753

Gene position: 2194672-2195847 (Clockwise)

Preceding gene: 209399582

Following gene: 209400318

Centisome position: 39.39

GC content: 50.77

Gene sequence:

>1176_bases
ATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAA
TCCGTCCAGCCAGCCGATGGCCGAGCTGTGGATGGGCGCACATCCGAAAAGCAGTTCACGAGTGCAGAATGCCGCCGGAG
ATATCGTTTCACTGCGTGATGTGATTGAGAGTGATAAATCGACTCTGCTCGGAGAGGCCGTTGCCAAACGCTTTGGCGAA
CTGCCTTTCCTGTTCAAAGTATTATGCGCAGCACAGCCACTCTCCATTCAGGTTCATCCAAACAAACACAAGTCTGAAAT
CGGTTTTGCCAAAGAAAATGCCGCAGGTATCCCGATGGATGCCGCCGAGCGTAACTATAAAGATCCTAACCACAAGCCGG
AGCTGGTTTTTGCGCTGACGCCTTTCCTTGCGATGAACGCGTTTCGTGAATTTTCCGAGATTGTCTCCCTACTCCAGCCG
GTCGCAGGTGCACATCCGGCGATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCT
GTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCGCTCGACAGTCAGCAGGGTGAACCGT
GGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGATGACAGCGGCCTGTTCTCTCCGCTGTTGCTGAATGTGGTGAAA
TTAAATCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGC
AAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAGTTCG
AAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCTGTTGATGAT
TTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGA
AGGCGATGCAACGTTGTGGAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAACGAAT
CACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCACGGGTTTACAACAAGCTGTAA

Upstream 100 bases:

>100_bases
AAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGCGGGATTAATTTCCA
CATTAAAACAGGGATTGATC

Downstream 100 bases:

>100_bases
GAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATT
TCATTTTATGGATAATCATT

Product: mannose-6-phosphate isomerase, class I

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKHKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Sequences:

>Translated_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKHKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL
>Mature_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKHKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=406, Percent_Identity=34.2364532019704, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1787899, Length=391, Percent_Identity=99.4884910485933, Blast_Score=796, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997620, Length=394, Percent_Identity=31.2182741116751, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17557650, Length=419, Percent_Identity=29.1169451073986, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=419, Percent_Identity=31.980906921241, Blast_Score=179, Evalue=6e-46,
Organism=Drosophila melanogaster, GI21356061, Length=400, Percent_Identity=31.5, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 42850; Mature: 42850

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCHHHHHHH
VIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKHKSEIGFAKENAAGIPMD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCEECCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC
>Mature Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCHHHHHHH
VIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKHKSEIGFAKENAAGIPMD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCEECCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]