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The map label for this gene is hisB

Identifier: 209400725

GI number: 209400725

Start: 2730368

End: 2731435

Strand: Direct

Name: hisB

Synonym: ECH74115_2955

Alternate gene names: 209400725

Gene position: 2730368-2731435 (Clockwise)

Preceding gene: 209396323

Following gene: 209396111

Centisome position: 49.0

GC content: 53.28

Gene sequence:

>1068_bases
ATGAGTCAGAAGTATCTTTTTATCGATCGCGATGGAACCCTGATTAGCGAACCGCCGAGTGATTTTCAGGTGGATCGTTT
TGACAAACTCGCCTTTGAACCGGGCGTGATCCCGCAACTGCTGAAGCTGCAAAAAGCGGGCTACAAACTGGTGATGATCA
CTAATCAGGATGGTCTGGGAACACAAAGTTTCCCGCAGGCGGATTTCGATGGCCCGCACAACCTGATGATGCAGATATTC
ACCTCGCAAGGCGTACAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAACCGAA
AGTAAAACTGGTGGAGCGTTATCTCGCTGAGCAAGCGATGGATCGCGCCAACAGTTATGTGATTGGCGATCGCGCGACCG
ATATTCAACTCGCTGAAAACATGGGCATTAATGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAG
CAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGA
TCGTGAAGGTGGCAGCAAGATTAATACCGGCGTTGGCTTCTTTGATCACATGCTGGATCAGATCGCCACCCACGGCGGTT
TCCGTATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGC
GAAGCGTTAAAAATTGCCCTCGGCGATAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTGCCGATGGACGAATGCCTTGC
CCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAACGCGTGGGCGATCTCA
GCACCGAGATGATCGAGCACTTCTTCCGTTCGCTCTCTTACACCATGGGCGTGACGCTCCACCTGAAAACCAAAGGTAAA
AACGATCATCACCGTGTAGAGAGCCTGTTCAAAGCCTTTGGTCGGACCCTGCGCCAGGCCATCCGCGTGGAAGGCGATAC
CCTGCCCTCGTCGAAAGGAGTGCTGTAA

Upstream 100 bases:

>100_bases
AGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCC
TTACGTGCGGAGCAAGTTTG

Downstream 100 bases:

>100_bases
TGAACGTGGTGATCCTTGATACTGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCC
AAAGTCAGCCGTGACCCGGA

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQKAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIF
TSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGK
NDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL

Sequences:

>Translated_355_residues
MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQKAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIF
TSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGK
NDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL
>Mature_354_residues
SQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQKAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFT
SQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGEQ
LTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALGE
ALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKN
DHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=355, Percent_Identity=99.4366197183099, Blast_Score=735, Evalue=0.0,
Organism=Escherichia coli, GI1786399, Length=154, Percent_Identity=32.4675324675325, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324776, Length=165, Percent_Identity=47.8787878787879, Blast_Score=159, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_ECO57 (Q9S5G5)

Other databases:

- EMBL:   AB008676
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85827
- PIR:   G90981
- RefSeq:   NP_288527.2
- RefSeq:   NP_310850.2
- PDB:   2FPR
- PDB:   2FPS
- PDB:   2FPU
- PDB:   2FPW
- PDB:   2FPX
- PDBsum:   2FPR
- PDBsum:   2FPS
- PDBsum:   2FPU
- PDBsum:   2FPW
- PDBsum:   2FPX
- ProteinModelPortal:   Q9S5G5
- SMR:   Q9S5G5
- EnsemblBacteria:   EBESCT00000027077
- EnsemblBacteria:   EBESCT00000055491
- GeneID:   914091
- GeneID:   962080
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3184
- KEGG:   ecs:ECs2823
- GeneTree:   EBGT00050000010149
- HOGENOM:   HBG289010
- OMA:   QDGLGTP
- ProtClustDB:   PRK05446
- BioCyc:   ECOL83334:ECS2823-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01022
- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01662
- TIGRFAMs:   TIGR01261
- TIGRFAMs:   TIGR01656

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56784 SSF56784

EC number: =3.1.3.15; =4.2.1.19

Molecular weight: Translated: 40291; Mature: 40159

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQKAGYKLVMITNQDGLG
CCCCEEEEECCCCEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCEEEEEEECCCCCC
TQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAM
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
DRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGEQLTRRDRYAHVVRNTKETQI
HHCCCEEECCCCCCEEEHHCCCCCCEEECHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEE
DVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCHHHHHHHH
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEH
HHEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCHHHHHHHH
FFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL
HHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQKAGYKLVMITNQDGLG
CCCEEEEECCCCEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCEEEEEEECCCCCC
TQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAM
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
DRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGEQLTRRDRYAHVVRNTKETQI
HHCCCEEECCCCCCEEEHHCCCCCCEEECHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEE
DVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCHHHHHHHH
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEH
HHEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCHHHHHHHH
FFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL
HHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796