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The map label for this gene is nupC [H]

Identifier: 209400703

GI number: 209400703

Start: 3345291

End: 3346493

Strand: Direct

Name: nupC [H]

Synonym: ECH74115_3624

Alternate gene names: 209400703

Gene position: 3345291-3346493 (Clockwise)

Preceding gene: 209399524

Following gene: 209399965

Centisome position: 60.04

GC content: 49.38

Gene sequence:

>1203_bases
ATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATCCTCGCACTGCTGGTAAGCAGCGACCGCAAAAA
AATTCGTATCCGTTATGTTATTCAACTGCTTGTTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTT
TAGGCTTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGT
AGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTAT
TCTCCAGCACATTCGCGTGTTGCCGGTGATTATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAAC
TGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTATTGCCTATAAAGATATCCTTGGCAAA
ATCTCCCGTAACCGTATGTACACCATGGCAGCAACGGCGATGTCCACCGTTTCTATGTCCATCGTTGGTGCATACATGAC
CATGCTGGAACCAAAATACGTCGTTGCAGCGCTGGTACTGAACATGTTCAGCACCTTTATCGTGCTGTCGCTGATCAACC
CTTACCGTGTTGATGCCTGTGAAGAAAACATTCAGATGTCCAACCTGCACGAAGGTCAGAGCTTCTTCGAAATGCTGGGT
GAATACATTCTGGCAGGTTTCAAAGTTGCCATTATCGTTGCCGCAATGCTGATTGGCTTTATCGCCCTGATCGCCGCGCT
GAACGCACTGTTTGCTACCGTGACTGGCTGGATTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATC
CGATTGCATGGGTGATGGGTGTTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAAC
GAGTTCGTTGCGATGATGGATCTTCAGAAAATTGCCTCTACACTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCT
GGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGG
TTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTG
TAA

Upstream 100 bases:

>100_bases
TCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCT
GCCTCATATTTGGAGCAAAT

Downstream 100 bases:

>100_bases
GACCATACATAAAAAAGCCGGGGATAATTCCCCGGCTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATC
AAATACCCTTGCAGATATTG

Product: nucleoside transporter NupC

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDACEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL

Sequences:

>Translated_400_residues
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDACEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
>Mature_400_residues
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDACEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=394, Percent_Identity=25.3807106598985, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI11545853, Length=406, Percent_Identity=23.8916256157635, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI227116277, Length=403, Percent_Identity=21.8362282878412, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=99.5, Blast_Score=783, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=414, Percent_Identity=31.8840579710145, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788488, Length=412, Percent_Identity=30.0970873786408, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560276, Length=393, Percent_Identity=24.6819338422392, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=392, Percent_Identity=25.5102040816327, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45552519, Length=391, Percent_Identity=25.3196930946292, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45552517, Length=391, Percent_Identity=25.3196930946292, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19921868, Length=391, Percent_Identity=25.3196930946292, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281360430, Length=344, Percent_Identity=25, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43458; Mature: 43458

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDACEENIQMSNLHEGQSFFEMLG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCHHHHHHHHH
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWIGYSISFQGILGYIFYPIAWVMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHH
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDACEENIQMSNLHEGQSFFEMLG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCHHHHHHHHH
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWIGYSISFQGILGYIFYPIAWVMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHH
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]