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The map label for this gene is ubiF [H]

Identifier: 209400601

GI number: 209400601

Start: 778537

End: 779712

Strand: Direct

Name: ubiF [H]

Synonym: ECH74115_0756

Alternate gene names: 209400601

Gene position: 778537-779712 (Clockwise)

Preceding gene: 209398523

Following gene: 209399473

Centisome position: 13.97

GC content: 56.29

Gene sequence:

>1176_bases
ATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCA
CGGATTTACGGTAACGGTGATCGAGCACGCAGAACCTGCACCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGG
CGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATGCGTTGCCATCCTTAC
CGCAGACTGGAAACGTGGGAGTGGGAAACGGCGCATGTGGTATTTGACGCAGCCGAATTAAAGTTGCCGTTGCTTGGCTA
TATGGTAGAAAACACTGTCCTGCAACAGGCATTGTGGCAGGCGCTGGAAGCGCATCCGAAAGTAACGTTACGTGTGCCAA
CCTCGCTGATTGCATTACATCGAGATAATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTG
GTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTC
GTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGATAGCACCTGGCAGCAATTTACCCCGGATGGACCGCGCG
CGTTTCTGCCGCTGTTTGATAACTGGGCATCGCTGGTGTGGTACGACTCTCCGGCGCGCATTCGCCAGTTGCAGAATATG
AATATGGCGCAGTTACAGGTGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGC
GTTTCCGCTGACACGCCGCCATGCGTTGCAGTATGTGCAGCCGGGGCTTGCGCTGGTGGGTGATGCCGCGCACACCATCC
ATCCGCTGGCGGGGCAGGGGGTGAATCTTGGTTATCGTGATGTCGATGCCCTGATTGATGTTCTGGTGAATGCCCGCAGC
TACGGCGAAGCGTGGGCCAGTTATCCTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAGAG
CGGTATGGATCTGTTTTATGCCGGATTCAGTAATAATCTGCCGCCGCTGCGTTTTGTGCGTAATCTTGGTTTGATGGCGG
CGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAG

Upstream 100 bases:

>100_bases
TGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTGGGGGTAAAAATCCTGTAAACTTAAAGCATAT
TGCTAACAGGATGATTGACC

Downstream 100 bases:

>100_bases
CCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGA
CGTGTCAACATCGCATTCGA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVADSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPY
RRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
NMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARS
YGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL

Sequences:

>Translated_391_residues
MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVADSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPY
RRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
NMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARS
YGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL
>Mature_390_residues
TNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVADSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYR
RLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKLV
IGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNMN
MAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSY
GEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=390, Percent_Identity=32.3076923076923, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI32967307, Length=349, Percent_Identity=31.8051575931232, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=98.4654731457801, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=38.7755102040816, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1789274, Length=372, Percent_Identity=32.258064516129, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=430, Percent_Identity=28.1395348837209, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321694, Length=451, Percent_Identity=25.7206208425721, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920760, Length=378, Percent_Identity=28.5714285714286, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43001; Mature: 42870

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVADSQPDVRISAISAASV
CCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
SLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQ
HHHHHCCHHHHHHHHHCCCCHHCCCCCCCEEEEEEEHHHHCHHHHHHHHHHHHHHHHHHH
ALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKLVIGADGANSQVRQMAGIGVH
HHHCCCCEEEECCCEEEEEECCCCHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
HHHHHCCEEEEEEEECCCCCCCHHHHCCCCCCEEEHHHHCCCEEEEEECCHHHHHHHHCC
NMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
CHHHHHHHHHHHCHHHCCCCCHHHCCCCCHHHHHHHHHHCCCHHEECCCHHHCCCCCCCC
VNLGYRDVDALIDVLVNARSYGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNL
CCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRFVRNLGLMAAERAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVADSQPDVRISAISAASV
CCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
SLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQ
HHHHHCCHHHHHHHHHCCCCHHCCCCCCCEEEEEEEHHHHCHHHHHHHHHHHHHHHHHHH
ALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKLVIGADGANSQVRQMAGIGVH
HHHCCCCEEEECCCEEEEEECCCCHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
HHHHHCCEEEEEEEECCCCCCCHHHHCCCCCCEEEHHHHCCCEEEEEECCHHHHHHHHCC
NMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
CHHHHHHHHHHHCHHHCCCCCHHHCCCCCHHHHHHHHHHCCCHHEECCCHHHCCCCCCCC
VNLGYRDVDALIDVLVNARSYGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNL
CCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRFVRNLGLMAAERAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]