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The map label for this gene is ybeM

Identifier: 209400594

GI number: 209400594

Start: 742661

End: 743449

Strand: Direct

Name: ybeM

Synonym: ECH74115_0714

Alternate gene names: 209400594

Gene position: 742661-743449 (Clockwise)

Preceding gene: 209399192

Following gene: 209399866

Centisome position: 13.33

GC content: 54.12

Gene sequence:

>789_bases
ATGCTGGTTGCTGCAGGACAGTTTGCTGTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCA
GGCGGCGGAAAACGACGTATCGCTGTTTGTCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGG
TTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACGTTTACGGCGAGAAAGTAAACGTAACATGATGACGACAATT
CTGACGATTCATGTTCCTTCAACGCCGGGGCGCGCATGGAATATGCTGGTGGCGCTACAGGCAGGAAACATCGTCGCCCG
TTATGCCAAATTGCATCTCTATGATGCATTTGCCATTCAGGAATCACGCCGTGTTGATGCTGGTAATGAAATTGCTCCGT
TACTCGAGGTGGAGGGGATGAAGGTCGGTCTGATGACCTGTTATGACTTACGCTTTCCAGAGCTGGCGCTGGCACAGGCA
TTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCACCACTGGTCAACGTTGCT
TGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGGCAAAGCCGGA
TTATAGATCCCTTTGGCGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGT
GTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGA

Upstream 100 bases:

>100_bases
TGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTGATAAGTGTAACGCCGAATAATCGTCGTTGGCGAATTTTAC
GACTCTGACAGGAGGTGGCA

Downstream 100 bases:

>100_bases
TGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTTT
GGTTTTTAAGGCGCGTTCTG

Product: hydrolase, carbon-nitrogen family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLARDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTI
LTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPER
VRQVRAQLPVLNNRRFAPPQLL

Sequences:

>Translated_262_residues
MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLARDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTI
LTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPER
VRQVRAQLPVLNNRRFAPPQLL
>Mature_262_residues
MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLARDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTI
LTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPER
VRQVRAQLPVLNNRRFAPPQLL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI5031947, Length=279, Percent_Identity=35.4838709677419, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI297632348, Length=279, Percent_Identity=35.4838709677419, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI297632350, Length=279, Percent_Identity=35.4838709677419, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI9910460, Length=262, Percent_Identity=25.9541984732824, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI297632346, Length=202, Percent_Identity=33.1683168316832, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556280, Length=272, Percent_Identity=29.7794117647059, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322335, Length=297, Percent_Identity=29.96632996633, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323383, Length=250, Percent_Identity=28, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21355835, Length=168, Percent_Identity=32.1428571428571, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17933642, Length=267, Percent_Identity=29.5880149812734, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBEM_ECO57 (P58054)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85562
- PIR:   H90711
- RefSeq:   NP_286352.1
- RefSeq:   NP_308691.1
- ProteinModelPortal:   P58054
- SMR:   P58054
- EnsemblBacteria:   EBESCT00000028488
- EnsemblBacteria:   EBESCT00000059185
- GeneID:   917024
- GeneID:   957658
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0771
- KEGG:   ecs:ECs0664
- GeneTree:   EBGT00050000010760
- HOGENOM:   HBG742418
- OMA:   VWEKNAQ
- BioCyc:   ECOL83334:ECS0664-MONOMER
- InterPro:   IPR003010
- InterPro:   IPR001110
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28682; Mature: 28682

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50263 CN_HYDROLASE; PS01227 UPF0012

Important sites: ACT_SITE 40-40 ACT_SITE 110-110 ACT_SITE 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLARDDHDADLSVKSAQLL
CEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCCEEHHHHHH
EGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQ
HHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
ESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKE
HCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECHHHHCCCHHH
HHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPER
HHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCHHH
VRQVRAQLPVLNNRRFAPPQLL
HHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLARDDHDADLSVKSAQLL
CEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCCEEHHHHHH
EGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQ
HHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
ESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKE
HCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECHHHHCCCHHH
HHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPER
HHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCHHH
VRQVRAQLPVLNNRRFAPPQLL
HHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796