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The map label for this gene is priC [H]

Identifier: 209400394

GI number: 209400394

Start: 559766

End: 560293

Strand: Reverse

Name: priC [H]

Synonym: ECH74115_0557

Alternate gene names: 209400394

Gene position: 560293-559766 (Counterclockwise)

Preceding gene: 209398756

Following gene: 209400374

Centisome position: 10.06

GC content: 58.33

Gene sequence:

>528_bases
GTGAAAACCGCCCTGCTGCTGGAAAAACTGGAAGGACAGCTCGCTACGCTGCGTCAGCGTTGTGCCCCGGTGTCACAGTT
CGCCACGCTAAGTGCTCGTTTCAACAGGCATCTTTTTCAGACTCGTGCGACAACACTACAGGCTTGTCTCGACAAGGCGG
GCGATAATCTGGCTGCGCTTCGTCATGCAGTTGAGCAACAGCAACTGCCGCAAGTCGCCTGGCTGGCAGAACATCTGGCG
GCGCAACTGGAGGCTATAGCGCGTGAAGCCACAGCCTGGTCGCTACGCGAGTGGGATAGCGCACCACCCCAAATTGCCCG
CTGGCAGCGTAAACGTATTCAGCATCAGGATTTTGAGCGGCGGCTACGTGAGATGGTTGCCGAACGTAGAGCCCGCCTGG
CGCGGGTGACCGATCTCGTGGAACAGCAAACGCTGCATCGTGAAGTGGAAGCCTATGAAGCGCGCCTGGCACGCTGCCGC
CATGCGCTGGAAAAAATCGAAAACAGGTTAGCGCGTTTAACCCGCTAG

Upstream 100 bases:

>100_bases
AGCCAATGATGATTAAAATGATTCGTTGCATATTGAGTGTTGTCAGCTTACAGAGTGGCTACTTTAGCATAACAATTATC
ATTTTCATTGAGGTCTTATC

Downstream 100 bases:

>100_bases
CAATGGAGAGAGTATGTCACTGGAAAATGCCCCTGACGATGTCAAACTGGCTGTCGATTTGATTGTGCTGCTGGAAGAAA
ATCAGATCCCAGCCCGCACC

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRATTLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Sequences:

>Translated_175_residues
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRATTLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR
>Mature_175_residues
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRATTLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=97.7142857142857, Blast_Score=335, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20388; Mature: 20388

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRATTLQACLDKAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAPPQIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRATTLQACLDKAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAPPQIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]