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The map label for this gene is puuR [H]

Identifier: 209400310

GI number: 209400310

Start: 1836859

End: 1837416

Strand: Direct

Name: puuR [H]

Synonym: ECH74115_1941

Alternate gene names: 209400310

Gene position: 1836859-1837416 (Clockwise)

Preceding gene: 209400761

Following gene: 209396804

Centisome position: 32.97

GC content: 50.72

Gene sequence:

>558_bases
ATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGC
CGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAA
AGCTGCTGAAGGTGTATGGTCTGTCACTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATT
AATCAGGACGATTTAATTGAGATGGGCAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCT
GGCGATGATCTTTGAAACGTACCAGCCGGGCACAACCACTGGGGAAAGAATTAAGCATCAGGGTGAGGAAATAGGCACTG
TACTGGAAGGTGAAATTATTCTGACGATTAATGGTCAGGATTACCACCTCGTCGCGGGGCAAAGTTATGCCATTAATACC
GGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAA

Upstream 100 bases:

>100_bases
CGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTA
CAGTTTAAGGAAATGCAAAT

Downstream 100 bases:

>100_bases
TCTTTTTGTTCTGTAAGCCGGGTAAGCGCAATGCGCCCGGCAATCCATAAAGAATGTCGCCAGAAATAAACGGGCATACG
GCCCGGGGATCTCTGCGCCC

Product: DNA-binding transcriptional repressor PuuR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVI
NQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
GIPHSFSNTSAGICRIISAHTPTTF

Sequences:

>Translated_185_residues
MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVI
NQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
GIPHSFSNTSAGICRIISAHTPTTF
>Mature_184_residues
SDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVIN
QDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINTG
IPHSFSNTSAGICRIISAHTPTTF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=185, Percent_Identity=99.4594594594595, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20106; Mature: 19975

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYG
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
LSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTT
CCHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCCCH
GERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINTGIPHSFSNTSAGICRIISAH
HHHHHHHHHHHCEEECCEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCHHHEEEECC
TPTTF
CCCCC
>Mature Secondary Structure 
SDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYG
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
LSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTT
CCHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCCCH
GERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINTGIPHSFSNTSAGICRIISAH
HHHHHHHHHHHCEEECCEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCHHHEEEECC
TPTTF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]