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The map label for this gene is mdtA [H]

Identifier: 209400133

GI number: 209400133

Start: 2794284

End: 2795531

Strand: Direct

Name: mdtA [H]

Synonym: ECH74115_3014

Alternate gene names: 209400133

Gene position: 2794284-2795531 (Clockwise)

Preceding gene: 209397796

Following gene: 209399550

Centisome position: 50.15

GC content: 55.37

Gene sequence:

>1248_bases
ATGAAAGGCAGTTATAAATCCCGTTGGGTAATCGTAATCGTGGTAGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCA
AGGCCGCAATGACTCCCAGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGGTGGTCGCCGTGGTA
TGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGC
ACCATTACCGCTGCTAATACTGTTACGGTGCGTAGCCGCGTGGACGGTCAACTGATGGCGTTACATTTCCAGGAAGGCCA
GCAGGTCAAAGCTGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAAC
TGGCAAAAGATAAAGCCACGCTTACCAACGCCCGCCGTGATCTGGCGCGTTATCAGCAACTGGCAAAAACCAATCTCGTC
TCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTCGC
CAGCGCGCAGCTGCAACTCGACTGGAGCCGTATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTG
GTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTGCTCTTTACCCTG
CCGGAAAGCGATATCGCTACCGTCGTGCAGGCGCAAAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAA
CTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTCGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAG
CACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAAC
GCCGTAGTGATCCCAACAGCCGCCCTGCAAATGGGCAATGAAGGCCATTTTGTCTGGGTGCTGAATAGCGAAAACAAGGT
CAGCAAACATCTGGTGACGCCGGGCATTCAGGACAGTCAGAAAGTGGTGATCCGCGCAGGTATTTCTGCGGGCGATCGCG
TGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACCACTCCGGAA
GAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCTCGCTCCTGA

Upstream 100 bases:

>100_bases
ATTCCCGATAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTC
AGGAAGAGAAACTCTTAACG

Downstream 100 bases:

>100_bases
TGCAAGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATG
GTGGCGATCTTACTCGCCGG

Product: multidrug efflux system subunit MdtA

Products: NA

Alternate protein names: Multidrug transporter mdtA [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLG
TITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLLFTL
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQN
AVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPE
EKATSREYAKKGARS

Sequences:

>Translated_415_residues
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLG
TITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLLFTL
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQN
AVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPE
EKATSREYAKKGARS
>Mature_415_residues
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLG
TITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLLFTL
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQN
AVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPE
EKATSREYAKKGARS

Specific function: The mdtABC tripartite complex confers resistance against novobiocin and deoxycholate [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=415, Percent_Identity=99.0361445783133, Blast_Score=828, Evalue=0.0,
Organism=Escherichia coli, GI1789929, Length=311, Percent_Identity=28.2958199356913, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786668, Length=395, Percent_Identity=28.3544303797468, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789665, Length=320, Percent_Identity=28.75, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081796, Length=394, Percent_Identity=26.3959390862944, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081951, Length=136, Percent_Identity=33.8235294117647, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022824
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44499; Mature: 44499

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQSPAGGRRGMRSGPLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
PVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
CHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEEEEEEECCCCEECCCCEEEECCH
SQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
ADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLLFTL
ECCCHHEEEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCEEEEEEC
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQD
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCC
DALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQ
CCCCCHHHCCEEEEEECCCCEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCC
KVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAKKGARS
EEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQSPAGGRRGMRSGPLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
PVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
CHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEEEEEEECCCCEECCCCEEEECCH
SQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
ADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLLFTL
ECCCHHEEEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCEEEEEEC
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQD
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCC
DALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQ
CCCCCHHHCCEEEEEECCCCEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCC
KVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAKKGARS
EEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA