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The map label for this gene is hcaF [H]

Identifier: 209400100

GI number: 209400100

Start: 3495008

End: 3495526

Strand: Direct

Name: hcaF [H]

Synonym: ECH74115_3771

Alternate gene names: 209400100

Gene position: 3495008-3495526 (Clockwise)

Preceding gene: 209400112

Following gene: 209397149

Centisome position: 62.72

GC content: 52.41

Gene sequence:

>519_bases
ATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGTTTCTCTTTCACGAAGCCAGCTTACTGGACGACTG
GAAATTTCGTGACTGGCTGGCACAGCTCGACGAAGAGATTTGTTACACCATGCGCACCACGGTGAACGCGCAAACGCGCG
ACCGCCGCAAAGGTGTTCAGCCGCCGACAACCTGGATTTTTAATGACACCAAAGACCAGCTGGAGCGGCGAATCGCCCGT
CTGGAAACGGGCATGGCATGGGCAGAAGAGCCGCCGTCACGCACCCGCCACTTAATCAGCAACTGCCAGATAAGCGAAAC
CGACATCCCAAACGTATTTGCTGTTCGGGTGAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACGTTCTATG
TCGGGACTCGTTTTGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAA
GCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGA

Upstream 100 bases:

>100_bases
CTGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGTGCTCGATAAAACCGCCGCT
TACCAGCAGGAGGTGATGAA

Downstream 100 bases:

>100_bases
TGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCC
TTGTTCAACGTTGGCGGCGA

Product: 3-phenylpropionate dioxygenase subunit beta

Products: NA

Alternate protein names: Digoxigenin subunit beta [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTMRTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIAR
LETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
AVITSHNLSVLF

Sequences:

>Translated_172_residues
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTMRTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIAR
LETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
AVITSHNLSVLF
>Mature_171_residues
SAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTMRTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARL
ETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQA
VITSHNLSVLF

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788889, Length=172, Percent_Identity=99.4186046511628, Blast_Score=351, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 20526; Mature: 20395

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTMRTTVNAQTRDRRKGVQ
CCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF
HHHHHHCCCCEEEEECHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
SAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTMRTTVNAQTRDRRKGVQ
CCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF
HHHHHHCCCCEEEEECHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA