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The map label for this gene is rusA [H]

Identifier: 209400068

GI number: 209400068

Start: 2146613

End: 2146972

Strand: Reverse

Name: rusA [H]

Synonym: ECH74115_2267

Alternate gene names: 209400068

Gene position: 2146972-2146613 (Counterclockwise)

Preceding gene: 209400833

Following gene: 209397345

Centisome position: 38.53

GC content: 51.67

Gene sequence:

>360_bases
ATGCGTATAGAGTTTGTTTTGCCTTACCCGCCGACGGTGAACACCTACTGGCGACGTCGTGGCAGCACATATTTTGTATC
AAAAGCCGGTGAGCGTTATCGCCGGGATGTGGCACTTATTGTTCGCCAGCAGCGGCTGAAATTAAACCTGTCCGGAAGGC
TGGTGATAAAGATTATTGCAGAGCCACCGGATAAGCGCCGTCGTGACCTGGACAATATCCTGAAAGCACCACTGGATGTG
CTGACGCATGCCGGACTTCTCATAGACGACGAGCAGTTTGATGAAATCAATATTGTGCGCGGTCAGCTCGTTCCTGGTGA
GCGGCTGGGGATAAAAATCACAGAACTGGAGTGCGCATGA

Upstream 100 bases:

>100_bases
GCTGGCGTTCGAAGAAAAGCATGGTTCCCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACCGGCGTGC
TCGGGTAAAAGAGGTTACTG

Downstream 100 bases:

>100_bases
ATAACCACTATTTACAGTTTGTGCGTGAGCAGCTCATTATCGCCACCGCTGATTTGAGTGGGGCAACAAAAGGTCAGCTT
GAAGCCTGGCAGGAGAATGC

Product: crossover junction endodeoxyribonuclease RusA homolog

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDV
LTHAGLLIDDEQFDEINIVRGQLVPGERLGIKITELECA

Sequences:

>Translated_119_residues
MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDV
LTHAGLLIDDEQFDEINIVRGQLVPGERLGIKITELECA
>Mature_119_residues
MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDV
LTHAGLLIDDEQFDEINIVRGQLVPGERLGIKITELECA

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 13811; Mature: 13811

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLVIKIIA
CEEEEEECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEEEC
EPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVRGQLVPGERLGIKITELECA
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLVIKIIA
CEEEEEECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEEEC
EPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVRGQLVPGERLGIKITELECA
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA