Click here to switch to the map view.

The map label for this gene is dinB [H]

Identifier: 209399967

GI number: 209399967

Start: 291345

End: 292400

Strand: Direct

Name: dinB [H]

Synonym: ECH74115_0276

Alternate gene names: 209399967

Gene position: 291345-292400 (Clockwise)

Preceding gene: 209397836

Following gene: 209400393

Centisome position: 5.23

GC content: 53.98

Gene sequence:

>1056_bases
ATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGCCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATAT
CCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTAC
GCAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACAAA
GAAGCCTCAAATCATATCCGTGAAATCTTCTCGCGCTACACCTCGCGTATTGAACCGTTGTCACTGGATGAAGCCTACCT
CGATGTCACTGATAGCGTCCATTGCCACGGTTCTGCGACCCTCATCGCCCAGGAAATCCGCCAGACAATTTTCAGCGAGC
TGCAACTGACGGCGTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGC
CAGTTTGTGATTACGCCGGCAGAAGTTTCGGCATTTCTGCAAACCTTACCGCTGGCAAAAATTCCCGGTGTTGGCAAAGT
CTCAGCGGCAAAGCTGGAAGCGATGGGGCTGCGGACCTGCGGTGATGTACAAAAGTGTGATCTGGTGATTCTGCTTAAAC
GCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAGCGCGACGTTAACAGCGAACGGTTACGA
AAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATTCATCACTGGTCTGAATGTGAAGCGATTATCGAGCGGCTGTA
TCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACG
ATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGACTTAATCGCCACCGCGCGTAAAACCTGG
GATGAACGCCGCGGCGGGCGCGGTGTGCGACTGGTGGGGCTGCATGTGACGTTGCTTGACCCTCAAATGGAAAGACAACT
GGTGCTGGGATTATGA

Upstream 100 bases:

>100_bases
CAGCAGGTGCTTTCGCAGCGAACGCGCTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTA
CCAGTATTGAGAGGTGAGTA

Downstream 100 bases:

>100_bases
TGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGGATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGT
CAATATCACTGAATTACGTA

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
DERRGGRGVRLVGLHVTLLDPQMERQLVLGL

Sequences:

>Translated_351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
DERRGGRGVRLVGLHVTLLDPQMERQLVLGL
>Mature_351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
DERRGGRGVRLVGLHVTLLDPQMERQLVLGL

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI7705344, Length=356, Percent_Identity=33.4269662921348, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI84043967, Length=324, Percent_Identity=31.1728395061728, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI7706681, Length=325, Percent_Identity=31.3846153846154, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI154350220, Length=251, Percent_Identity=32.6693227091633, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI5729982, Length=359, Percent_Identity=30.08356545961, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786425, Length=351, Percent_Identity=99.1452991452991, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1787432, Length=217, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193205700, Length=406, Percent_Identity=32.0197044334975, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537959, Length=290, Percent_Identity=29.3103448275862, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI193205702, Length=351, Percent_Identity=27.6353276353276, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115534089, Length=163, Percent_Identity=34.3558282208589, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324921, Length=208, Percent_Identity=30.2884615384615, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19923006, Length=327, Percent_Identity=32.1100917431193, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21355641, Length=356, Percent_Identity=29.2134831460674, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24644984, Length=356, Percent_Identity=29.2134831460674, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24668444, Length=121, Percent_Identity=37.1900826446281, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39461; Mature: 39461

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
CCCEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCHHHHCCHHCCCCC
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
CHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCHH
LIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVSAFLQTLPLAK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCHHH
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHH
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHH
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
CCCEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCHHHHCCHHCCCCC
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
CHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCHH
LIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVSAFLQTLPLAK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCHHH
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKFGRILWERSQGIDERDVNSERLR
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHH
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHH
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA