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The map label for this gene is gsiC

Identifier: 209399916

GI number: 209399916

Start: 998664

End: 999584

Strand: Direct

Name: gsiC

Synonym: ECH74115_0982

Alternate gene names: 209399916

Gene position: 998664-999584 (Clockwise)

Preceding gene: 209399726

Following gene: 209398764

Centisome position: 17.92

GC content: 53.96

Gene sequence:

>921_bases
ATGCTTAATTACGTTATCAAACGATTACTGGGGTTGATTCCGACGCTGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGT
GCATATGCTGCCCGGCGATCCGGCGCGATTGATTGCCGGGCCCGAAGCTGATGCGCAGGTTATAGAACTGGTGCGTCAGC
AGCTGGGGTTGGATCAGCCGCTGTATCACCAGTTCTGGCACTATATCAGCAATGCTGTGCAGGGGGATTTTGGCCTGTCG
ATGGTGTCACGTCGTCCGGTTGCCGATGAGATTGCCAGGCGCTTTATGCCAACGCTGTGGCTGACCATAACCAGTATGGT
CTGGGCGGTGATATTTGGTATGGCGGCGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGA
CCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCGCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGC
TGGCTGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGT
GATGGCGCGCTTTACCCGCGCGTCGTTTGTCGACGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGAAAGGGGTGA
GCGAAACCTGGGTTGTCCTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGC
TTTTTGCTCGGTGGTTCCATCGTGGTGGAGAAAGTTTTCAACTGGCCCGGACTTGGACGCTTACTCGTTGACTCCGTAGA
AATGCGCGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATG
TGCTTTACGCCGCCATTAACCCGGCTATCAGGTACAAGTAA

Upstream 100 bases:

>100_bases
TGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGATACCGGCTTCAGCTTTGAAGACGCGGATTTGCAA
TAAGCAACGCAGGGAGTGGA

Downstream 100 bases:

>100_bases
GGATGCGACTATTTAACTGGCGACGTCAGGCGGTGTTAAACGCCATGCCACTGGTCAAACCTGACCAGGTACGTACACCG
TGGCATGAATTCTGGCGACG

Product: glutathione ABC transporter, permease protein GsiC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEADAQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLS
MVSRRPVADEIARRFMPTLWLTITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWVVLKHGLRNAMIPVVTMMGLQFG
FLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQAEILLFSLEFILINLVVDVLYAAINPAIRYK

Sequences:

>Translated_306_residues
MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEADAQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLS
MVSRRPVADEIARRFMPTLWLTITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWVVLKHGLRNAMIPVVTMMGLQFG
FLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQAEILLFSLEFILINLVVDVLYAAINPAIRYK
>Mature_306_residues
MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEADAQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLS
MVSRRPVADEIARRFMPTLWLTITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWVVLKHGLRNAMIPVVTMMGLQFG
FLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQAEILLFSLEFILINLVVDVLYAAINPAIRYK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787053, Length=306, Percent_Identity=99.6732026143791, Blast_Score=609, Evalue=1e-176,
Organism=Escherichia coli, GI1789965, Length=342, Percent_Identity=42.1052631578947, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1789888, Length=308, Percent_Identity=40.5844155844156, Blast_Score=223, Evalue=9e-60,
Organism=Escherichia coli, GI1787761, Length=299, Percent_Identity=40.8026755852843, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1787497, Length=308, Percent_Identity=35.3896103896104, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1787550, Length=277, Percent_Identity=31.4079422382672, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788504, Length=363, Percent_Identity=26.4462809917355, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSIC_ECO57 (Q8X6V7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85593
- PIR:   F90742
- RefSeq:   NP_286595.1
- RefSeq:   NP_308937.1
- ProteinModelPortal:   Q8X6V7
- EnsemblBacteria:   EBESCT00000026655
- EnsemblBacteria:   EBESCT00000057857
- GeneID:   917654
- GeneID:   958122
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1055
- KEGG:   ecs:ECs0910
- GeneTree:   EBGT00050000009815
- HOGENOM:   HBG724229
- OMA:   LYRDRWV
- ProtClustDB:   PRK15081
- BioCyc:   ECOL83334:ECS0910-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 34136; Mature: 34136

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x26289554)-; HASH(0x246af9c8)-; HASH(0x261f1148)-; HASH(0x261de96c)-; HASH(0x261f1244)-; HASH(0x40662260)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEADAQVIELVRQQLGLDQP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHCCCCH
LYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLTITSMVWAVIFGMAAGIIA
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELGWLPTVGADSWQHYILPSLTL
HHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWVVLKHGLRNAMIPVVTMMGLQFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQAEILLFSLEFILINLVVDVLYAAIN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAIRYK
HHHCCH
>Mature Secondary Structure
MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEADAQVIELVRQQLGLDQP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHCCCCH
LYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLTITSMVWAVIFGMAAGIIA
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELGWLPTVGADSWQHYILPSLTL
HHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWVVLKHGLRNAMIPVVTMMGLQFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQAEILLFSLEFILINLVVDVLYAAIN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAIRYK
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796