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The map label for this gene is gapC

Identifier: 209399787

GI number: 209399787

Start: 1921811

End: 1922812

Strand: Reverse

Name: gapC

Synonym: ECH74115_2022

Alternate gene names: 209399787

Gene position: 1922812-1921811 (Counterclockwise)

Preceding gene: 209395969

Following gene: 209400143

Centisome position: 34.51

GC content: 47.8

Gene sequence:

>1002_bases
ATGAGTAAAGTTGGTATTAACGGTTTTGGTCGTATCGGTCGACTGGTGTTGCGTCGATTACTTGAAGTCAAAAGCAACAT
AGACGTTGTCGCTATTAATGATCTTACTTCCCCAAAAATTCTCGCCTATCTGCTGAAACATGATTCAAACTATGGTCCGT
TCCCCTGGAGCGTTGATTTTACGGAAGATTCACTTATCGTTGATGGGAAAAGTATCGCGGTTTACGCCGAAAAAGAGGCT
AAAAATATTCCGTGGAAAGCGAAAGGTGCAGAAATCATTGTCGAATGTACTGGCTTTTATACCTCCGCCGAGAAATCGCA
GGCGCATCTTGATGCTGGCGCGAAGAAGGTGTTGATTTCCGCCCCTGCAGGTGAAATGAAAACTATCGTTTATAAAGTCA
ATGACGACACTCTGGATGGCAACGACACCATTGTTTCCGTGGCATCATGCACTACTAACTGTCTTGCGCCGATGGCCAAA
GCCTTGCATGACAGTTTCGGGATAGAAGTCGGCACGATGACGACCATTCATGCCTATACTGGCACCCAGTCACTGGTGGA
TGGCCCGCGTGGTAAAGATTTACGTGCTTCACGCGCCGCGGCAGAAAATATCATTCCCCACACTACGGGCGCGGCAAAAG
CCATTGGTCTGGTGATCCCGGAACTGAGCGGCAAACTGAAAGGTCATGCGCAACGCGTGCCGGTGAAAACAGGTTCGGTC
ACTGAACTGGTATCGATTCTCGGAAAAAAAGTGACTGCCGAAGAGGTGAATAACGCACTTAAACAAGCAACCACCAATAA
CGAGTCATTTGGTTATACCGATGAAGAAATAGTCTCTTCCGATATCATTGGCAGCCATTTCGGTTCGGTGTTTGATGCCA
CGCAAACGGAAATTACCGCCGTGGGCGATTTACAACTGGTGAAAACGGTCGCCTGGTACGACAACGAATATGGCTTCGTC
ACGCAGCTTATTCGCACCCTCGAAAAATTCGCTAAACTCTGA

Upstream 100 bases:

>100_bases
TTACGTTTCACTGTAAGAATGGAGATTGCCCATCCCGCCATCCTGGTCTAAGCCTGGAAAGGATCAATTTTCATCCGAAC
GTTCCTGACAGGAGAAAACC

Downstream 100 bases:

>100_bases
CGCGCACAGGCGGAGGAAAAAACCTCCGCCTCTTTCACTCATTAAGACTGTAAATAAACCACCTGGGTCTGCAGATATTC
ATGCAGGCCATGTTTACCAT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH-C

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEA
KNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLVIPELSGKLKGHAQRVPVKTGSV
TELVSILGKKVTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFV
TQLIRTLEKFAKL

Sequences:

>Translated_333_residues
MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEA
KNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLVIPELSGKLKGHAQRVPVKTGSV
TELVSILGKKVTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFV
TQLIRTLEKFAKL
>Mature_332_residues
SKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAK
NIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAKA
LHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLVIPELSGKLKGHAQRVPVKTGSVT
ELVSILGKKVTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVT
QLIRTLEKFAKL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=46.5256797583082, Blast_Score=284, Evalue=6e-77,
Organism=Homo sapiens, GI7669492, Length=332, Percent_Identity=44.578313253012, Blast_Score=278, Evalue=7e-75,
Organism=Escherichia coli, GI1788079, Length=333, Percent_Identity=46.2462462462462, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI1789295, Length=330, Percent_Identity=40.3030303030303, Blast_Score=236, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=46.2908011869436, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=46.2908011869436, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17534677, Length=335, Percent_Identity=47.4626865671642, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17534679, Length=335, Percent_Identity=46.865671641791, Blast_Score=273, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6321631, Length=336, Percent_Identity=45.5357142857143, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6322468, Length=339, Percent_Identity=46.3126843657817, Blast_Score=289, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6322409, Length=336, Percent_Identity=44.9404761904762, Blast_Score=285, Evalue=5e-78,
Organism=Drosophila melanogaster, GI19922412, Length=327, Percent_Identity=46.177370030581, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI17933600, Length=331, Percent_Identity=47.1299093655589, Blast_Score=284, Evalue=5e-77,
Organism=Drosophila melanogaster, GI18110149, Length=331, Percent_Identity=47.1299093655589, Blast_Score=284, Evalue=5e-77,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=46.8277945619335, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=46.8277945619335, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): G3P3_ECO57 (P58072)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85737
- PIR:   F90881
- RefSeq:   NP_287745.1
- RefSeq:   NP_310049.1
- ProteinModelPortal:   P58072
- SMR:   P58072
- EnsemblBacteria:   EBESCT00000028829
- EnsemblBacteria:   EBESCT00000056326
- GeneID:   917219
- GeneID:   960993
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2304
- KEGG:   ecs:ECs2022
- GeneTree:   EBGT00050000009772
- HOGENOM:   HBG571736
- OMA:   AMKKYES
- ProtClustDB:   CLSK880079
- BioCyc:   ECOL83334:ECS2022-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 35763; Mature: 35632

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00071 GAPDH; PS00430 TONB_DEPENDENT_REC_1

Important sites: ACT_SITE 150-150 BINDING 34-34 BINDING 78-78 BINDING 180-180 BINDING 232-232 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYLLKHDSNYGPFPWSVDF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEC
TEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLIS
CCCCEEECCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEE
APAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYT
CCCCCEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEECCEEEEEEEC
GTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLVIPELSGKLKGHAQRVPVKTGSV
CCHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHEEECCCCCCCCCCCEECCCCCCCH
TELVSILGKKVTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITA
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
VGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL
ECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYLLKHDSNYGPFPWSVDF
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEC
TEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLIS
CCCCEEECCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEE
APAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYT
CCCCCEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEECCEEEEEEEC
GTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLVIPELSGKLKGHAQRVPVKTGSV
CCHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHEEECCCCCCCCCCCEECCCCCCCH
TELVSILGKKVTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITA
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
VGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL
ECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796