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The map label for this gene is fixC

Identifier: 209399776

GI number: 209399776

Start: 48589

End: 49875

Strand: Direct

Name: fixC

Synonym: ECH74115_0047

Alternate gene names: 209399776

Gene position: 48589-49875 (Clockwise)

Preceding gene: 209400372

Following gene: 209397556

Centisome position: 0.87

GC content: 54.86

Gene sequence:

>1287_bases
ATGTCCGAAGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGA
AGGGGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGCCAAGAACGTCACCGGCGGGCGTCTCTATGCCCACA
GCCTGGAACACATTATTCCTGGTTTCGCCGAATCCGCCCCGGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATG
ACGGAAAAGTCAGCGATGACTATGGACTACTGCAACGGTGACGAAACCTCGCCATCCCAGCGTTCTTACTCCGTTTTGCG
CAGTAAATTTGATGCCTGGCTGATGGAGCAGGCCGAAGAAGCGGGCGCGCAGTTAATTACCGGGATCCGCGTCGATAACC
TCGTACAGCGCGATGGCAAAGTCGTCGGTGTAGAAGCCGATGGCGATGTGATTGAAGCGAAAACGGTGATCCTTGCTGAT
GGAGTGAACTCCATCCTTGCCGAAAAGCTGGGGATGGCAAAACGCGTTAAACCGACGAATGTGGCGGTTGGCGTGAAGGA
ACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTATTTGCGG
GATCACCCACCGATGGCCTGATGGGCGGCGGTTTTCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGT
CTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAACATCCGGCCGTTGCACCGCT
GATCGCGGGTGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGCTGGTCG
GTGACGGCGTATTGATTGCCGGTGATGCCGCCGGAATGTGTATGAACCTCGGTTTTACCATTCGCGGTATGGATCTGGCG
ATTGCCGCCGGGGAAGCTGCAGCAAAAACCGTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTCGCGGA
ATATCGTCAGCATCTTGAGAGTGGCCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCCTTCCTTGATAACCCAC
GCATGTTTAGCGGCTACCCGGAACTGGCGGTAGGCGTGGCGCGTGACCTGTTCACCATTGACGGCAGTGCGCCGGAACTG
ATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTGATCAAGGATGGCATGAAAGGAGTGACCGT
TTTATGA

Upstream 100 bases:

>100_bases
GCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACTCTGGCAGGGCTGCATTTTGGTCCT
GCCGCTGACAGGGAGCTCTT

Downstream 100 bases:

>100_bases
CTTCTCCCGTCAATGTGGACGTCAAACTGGGCGTCAATAAATTCAATGTCGATGAAGAGCATCCGCACATTGTTGTGAAA
GCCGATGCCGATAAACAGGC

Product: putative oxidoreductase FixC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFM
TEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTDGLMGGGFLYTNENTLSLGLVCG
LHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYSAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLA
IAAGEAAAKTVLSAMKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFTIDGSAPEL
MRKKILRHGKKVGFINLIKDGMKGVTVL

Sequences:

>Translated_428_residues
MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFM
TEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTDGLMGGGFLYTNENTLSLGLVCG
LHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYSAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLA
IAAGEAAAKTVLSAMKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFTIDGSAPEL
MRKKILRHGKKVGFINLIKDGMKGVTVL
>Mature_427_residues
SEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMT
EKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADG
VNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTDGLMGGGFLYTNENTLSLGLVCGL
HHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYSAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAI
AAGEAAAKTVLSAMKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFTIDGSAPELM
RKKILRHGKKVGFINLIKDGMKGVTVL

Specific function: Could be part of an electron transfer system required for anaerobic carnitine reduction

COG id: COG0644

COG function: function code C; Dehydrogenases (flavoproteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF-QO/fixC family

Homologues:

Organism=Homo sapiens, GI119703746, Length=384, Percent_Identity=27.0833333333333, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1786227, Length=428, Percent_Identity=99.5327102803738, Blast_Score=868, Evalue=0.0,
Organism=Escherichia coli, GI1787991, Length=429, Percent_Identity=61.3053613053613, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI87082155, Length=428, Percent_Identity=40.4205607476636, Blast_Score=318, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25144720, Length=398, Percent_Identity=25.8793969849246, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324932, Length=376, Percent_Identity=27.1276595744681, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24652256, Length=365, Percent_Identity=28.4931506849315, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXC_ECO57 (Q7AHT0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F85485
- PIR:   F90634
- RefSeq:   NP_285738.1
- RefSeq:   NP_308073.1
- ProteinModelPortal:   Q7AHT0
- SMR:   Q7AHT0
- EnsemblBacteria:   EBESCT00000024071
- EnsemblBacteria:   EBESCT00000055907
- GeneID:   913445
- GeneID:   956714
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0049
- KEGG:   ecs:ECs0046
- GeneTree:   EBGT00050000009863
- HOGENOM:   HBG295204
- OMA:   VNIARDL
- ProtClustDB:   PRK10157
- BioCyc:   ECOL83334:ECS0046-MONOMER
- GO:   GO:0006810
- InterPro:   IPR006076
- InterPro:   IPR003042
- PRINTS:   PR00420

Pfam domain/function: PF01266 DAO

EC number: NA

Molecular weight: Translated: 45717; Mature: 45585

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIP
CCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
GFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEE
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADGVNSILAEKLGMAKRVKPTN
HHHHHHHCCHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCHHHHHHHHHCHHHCCCCCH
VAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTDGLMGGGFLYTNENTLSLGLVCG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEECCCCEEHHHHHH
LHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYSAHVVPEAGINMLPELVGDGVLIA
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCHHHHHHHHHCCCEEEE
GDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSAMKSDDFSKQKLAEYRQHLESGPLRDM
CCCCCHHEECCEEEECCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
RMYQKLPAFLDNPRMFSGYPELAVGVARDLFTIDGSAPELMRKKILRHGKKVGFINLIKD
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
GMKGVTVL
CCCCCCCC
>Mature Secondary Structure 
SEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIP
CHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
GFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEE
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADGVNSILAEKLGMAKRVKPTN
HHHHHHHCCHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCHHHHHHHHHCHHHCCCCCH
VAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTDGLMGGGFLYTNENTLSLGLVCG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEECCCCEEHHHHHH
LHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYSAHVVPEAGINMLPELVGDGVLIA
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCHHHHHHHHHCCCEEEE
GDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSAMKSDDFSKQKLAEYRQHLESGPLRDM
CCCCCHHEECCEEEECCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
RMYQKLPAFLDNPRMFSGYPELAVGVARDLFTIDGSAPELMRKKILRHGKKVGFINLIKD
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
GMKGVTVL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a quinone or similar compound as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796