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The map label for this gene is potI [H]

Identifier: 209399758

GI number: 209399758

Start: 1025734

End: 1026579

Strand: Direct

Name: potI [H]

Synonym: ECH74115_1013

Alternate gene names: 209399758

Gene position: 1025734-1026579 (Clockwise)

Preceding gene: 209396848

Following gene: 209397452

Centisome position: 18.41

GC content: 54.85

Gene sequence:

>846_bases
ATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGTTGGGCTTCACCTTTCTCTACGCGCCAAT
GCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGCTGGTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGCG
AATTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCG
ATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCAC
CGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCT
GGCCTGCGGACCGCGGTATGCTTACCATCTGGCTGGCGCATGTCACTTTCTGTACGGCTTATGTGGCAGTCGTTATTTCG
TCGCGTCTTCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGT
CATTACGCTACCGATGATCATGCCCGCGGTCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGA
TCGCCAGCTTTGTATCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCG
GAAATCAACGCCCTAGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGC
AGAAAAACAGCGGATACGCGACATCCAGTGTGCAAGACGTGGCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTTCACAAACACCAGCAA
AAAAGCGTGGGAGAACACGG

Downstream 100 bases:

>100_bases
AGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGTATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGA
TTTTTTAAGAAAACATCTTC

Product: putrescine transporter subunit: membrane component of ABC superfamily

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQCARRG

Sequences:

>Translated_281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQCARRG
>Mature_281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQCARRG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=281, Percent_Identity=99.288256227758, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=41.0041841004184, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=31.25, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=27.3584905660377, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30474; Mature: 30474

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQCARRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQCARRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]