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The map label for this gene is yebU [H]

Identifier: 209399709

GI number: 209399709

Start: 2428348

End: 2429787

Strand: Direct

Name: yebU [H]

Synonym: ECH74115_2568

Alternate gene names: 209399709

Gene position: 2428348-2429787 (Clockwise)

Preceding gene: 209400762

Following gene: 209396659

Centisome position: 43.58

GC content: 52.5

Gene sequence:

>1440_bases
GTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACGCAAATGCGCGAAGCAATGCCTTCGACGCTCTCTTTTGA
TGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGCATTCGCGTTAATACGCTGAAAACCTCCGTTGCTGATTTCC
TGCGATTAACCGCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACGAT
GAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAGGAAGCCAGTTCAATGTTGCC
CGTCGCCGCCTTGTTTGCTGACGGTAATGCACCACAGCGGGTGATGGATGTCGCTGCTGCGCCAGGCTCCAAAACGACGC
AAATTGCCGCGCGGATGAATAACGAAGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCC
AATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCATTGCCAGAAAT
GTTCGATGCCATTTTGCTGGACGCTCCCTGCTCCGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAATTGGT
CACCAGAAAGCAATCAGGAAATCGCAGCTACCCAACGGGAACTGATCGACAGTGCCTTTCATGCATTACGCTCTGGCGGT
ACACTGGTTTACTCGACCTGTACCTTAAACCGGGAAGAAAACGAATCCGTTTGCCTGTGGCTTAAAGAGACTTACCCCGA
CGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTT
TCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCCATTCCCGCCTTACCCACCCCA
AAATACAAAGTGGGCAATTTCCCGTTCAGCCCGGTGAAAGATCGCGAAGCCGCACAAATTCGTCAGGCGGCTGCAAGTGT
TGGCTTAAACTGGGATGGAAACCTGCGACTCTGGCAGCGCGACAAAGAAGTGTGGTTGTTTCCGGTAGGCATTGAAGCCC
TGATCGGTAAAGTCCGATTTTCTCGCCTGGGGATTAAACTTGCCGAAACGCACAACAAAGGTTATCGCTGGCAGCATGAA
GCGGTTATTGCCCTTGCCTCCCCCGACAACGAGAACGCTTTTGAACTGACACCGCAAGAAGCGGAGGAGTGGTATCGCGG
GCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGACGTGTTGGTTACTTTCCAGCATCAGCCGATTGGTTTAGCCA
AACGGATTGGTTCGCGACTGAAAAACAGCTACCCGCGTGAACTGGTGCGGGACGGGAAACTTTTTACCAGTAACGCATGA

Upstream 100 bases:

>100_bases
GAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT
TTTGCCAGTGGTATATGCTC

Downstream 100 bases:

>100_bases
CAGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCGGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGG
TCGTACCACAACCGGCAGCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDD
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRSGG
TLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTP
KYKVGNFPFSPVKDREAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSNA

Sequences:

>Translated_479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDD
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRSGG
TLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTP
KYKVGNFPFSPVKDREAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSNA
>Mature_478_residues
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDE
DALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHAN
ISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRSGGT
LVYSTCTLNREENESVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPK
YKVGNFPFSPVKDREAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEA
VIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSNA

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=306, Percent_Identity=36.9281045751634, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI76150623, Length=306, Percent_Identity=36.9281045751634, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=37.7245508982036, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI39995082, Length=287, Percent_Identity=27.8745644599303, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI301336155, Length=287, Percent_Identity=27.8745644599303, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI32698918, Length=160, Percent_Identity=35, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI270288816, Length=348, Percent_Identity=29.3103448275862, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI23199998, Length=354, Percent_Identity=28.8135593220339, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI270288818, Length=319, Percent_Identity=29.4670846394984, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI8922322, Length=319, Percent_Identity=29.4670846394984, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI40316918, Length=210, Percent_Identity=30, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=96.6597077244259, Blast_Score=963, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=164, Percent_Identity=35.9756097560976, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=33.125, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=287, Percent_Identity=27.8745644599303, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=322, Percent_Identity=26.3975155279503, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=28.82096069869, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=36.4864864864865, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=207, Percent_Identity=31.4009661835749, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI22024126, Length=333, Percent_Identity=35.1351351351351, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=33.1730769230769, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=34.8571428571429, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=34.8571428571429, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53263; Mature: 53132

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLRLTAPY
CCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQR
CCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCCHH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRSGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKDREAAQIRQAAASVGLNWDGNLRLWQR
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEE
DKEVWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNENAFELTPQE
CCEEEEEECCHHHHHHHHHHHHHCEEEEECCCCCCEEECEEEEEEECCCCCCCEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC
>Mature Secondary Structure 
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLRLTAPY
CCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQR
CCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCCHH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRSGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKDREAAQIRQAAASVGLNWDGNLRLWQR
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEE
DKEVWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNENAFELTPQE
CCEEEEEECCHHHHHHHHHHHHHCEEEEECCCCCCEEECEEEEEEECCCCCCCEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA