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The map label for this gene is rtcR [H]

Identifier: 209399613

GI number: 209399613

Start: 4374295

End: 4375893

Strand: Direct

Name: rtcR [H]

Synonym: ECH74115_4731

Alternate gene names: 209399613

Gene position: 4374295-4375893 (Clockwise)

Preceding gene: 209399993

Following gene: 209399181

Centisome position: 78.5

GC content: 54.85

Gene sequence:

>1599_bases
ATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGAACAGTGCTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCGAAATG
GCGTCCGTCACTCTGTATATGCCAGCAAGAATCGTTAGTCATCGATCGACTGGAATTATTGCATGACGCCCGCTCGCGCT
CGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACCGAAGTGGTGAGCGTTGAGATTGAACTGCAT
AACCCGTGGGATTTCGAAGAGGTTTACGCCTGCCTGCATGATTTCGCCCGTGGTTACGAGTTTCAGCCAGAAAAAGAAGA
CTATTTAATTCACATCACCACCGGCACCCACGTCGCGCAGATTTGCTGGTTTCTGCTGGCTGAAGCGCGTTACCTGCCCG
CCCGGTTGATACAATCTTCACCTCCGCGCAAAAAAGAACAGCCGCGCGGCGCAGGTGAAGTGACGATTATCGATCTCGAT
TTAAGCCTTTATAACGCCATCGCCAGCCGCTTTGCCGAGGAACGCCAGCAAACGCTTGATTTTCTTAAGTCCGGTATTGC
CACGCGTAACCCCCACTTCAACCGCATGATTGAGCAGATTGAAAAAGTAGCGATTAAATCCCGCGCACCGATCCTGCTAA
ACGGCCCAACCGGTGCGGGCAAGTCATTTCTGGCGCGACGTATATTTGAATTAAAACAGGCGCGGCATCAGTTTAGCGGC
GCGTTTGTGGAAGTGAACTGCGCAACCCTGCGCGGCGATACCGCCATGTCGACGCTGTTTGGTCATGTGAAAGGCGCGTT
TACCGGGGCACGGGAATCTCGTGAAGGATTATTACGCAGCGCCAACGGCGGAATGTTGTTTCTTGATGAGATTGGCGAAC
TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAAAAAACCTTTTATCCGTTTGGCAGCGATCGCCAGGTG
AGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGTGATTTGCGCCAGCTGGTTGCCGATGGCAAATTCCGCGAAGATCT
ATACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGGCTACGCCAGCGTCAGGAAGATATTGAACCTAACCTGGATT
ATGAAGTGGAGCGCCACGCTTCACTCACCGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCCTTT
GCGACATCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAGCTTTCTGCCAGCGTCACGCGAATGGCAACCTTTGCCTC
TAGCGGACGCATTACTCTGGACGTGGTTGAAGATGAGATAAACCGTCTGCGCTATAACTGGCAGGAGAGCCGCCCCTCCG
CGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACACGTTATCGCAATC
TGCCGCCAGGCAAAGTCGCTTTCCGCAGCCGGACGCCAGCTTTTTGACGTTTCGCGCCAGGGCAAAGCCAGCGTCAATGA
CGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGA

Upstream 100 bases:

>100_bases
ATTGTTTTAAAAAGATTATATCTTTACGTCCGTAAACGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGT
CTTTTATAAGGATATCTAAG

Downstream 100 bases:

>100_bases
ATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGTGTCGGTGTAGACGGCATCTACCATGCTGATGCT
ACCCATATTGACCATCGCGT

Product: sigma-54 dependent transcriptional regulator RtcR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRLELLHDARSRSLFETLKRDIASVSPETEVVSVEIELH
NPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAI
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS

Sequences:

>Translated_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRLELLHDARSRSLFETLKRDIASVSPETEVVSVEIELH
NPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAI
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS
>Mature_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRLELLHDARSRSLFETLKRDIASVSPETEVVSVEIELH
NPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAI
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS

Specific function: Transcriptional repressor of the rtcAB genes. Interacts with sigma-54 [H]

COG id: COG4650

COG function: function code KT; Sigma54-dependent transcription regulator containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789828, Length=532, Percent_Identity=98.6842105263158, Blast_Score=1082, Evalue=0.0,
Organism=Escherichia coli, GI1788905, Length=282, Percent_Identity=36.5248226950355, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788550, Length=248, Percent_Identity=38.3064516129032, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1790437, Length=256, Percent_Identity=39.0625, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1789233, Length=264, Percent_Identity=35.2272727272727, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082117, Length=243, Percent_Identity=36.2139917695473, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1789087, Length=323, Percent_Identity=33.1269349845201, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787583, Length=244, Percent_Identity=36.0655737704918, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082152, Length=247, Percent_Identity=36.4372469635627, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1790299, Length=293, Percent_Identity=32.4232081911263, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1786524, Length=241, Percent_Identity=36.0995850622407, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87081872, Length=254, Percent_Identity=32.2834645669291, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI87081858, Length=242, Percent_Identity=29.7520661157025, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078
- InterPro:   IPR009715
- InterPro:   IPR017183 [H]

Pfam domain/function: PF06956 RtcR; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60223; Mature: 60223

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRLELLHDARSRSLFETLK
CCCEEEHHHHHHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQ
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSLYNAIASRFAEERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVADGKFREDLYARINLWTFTLPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHCCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHH
RMATFASSGRITLDVVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAI
HHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRLELLHDARSRSLFETLK
CCCEEEHHHHHHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQ
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSLYNAIASRFAEERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVADGKFREDLYARINLWTFTLPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHCCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHH
RMATFASSGRITLDVVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAI
HHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3045764; 7984428; 7920643; 9738023 [H]