| Definition | Escherichia coli O157:H7 str. EC4115, complete genome. |
|---|---|
| Accession | NC_011353 |
| Length | 5,572,075 |
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The map label for this gene is metL [H]
Identifier: 209399480
GI number: 209399480
Start: 5039830
End: 5042262
Strand: Direct
Name: metL [H]
Synonym: ECH74115_5400
Alternate gene names: 209399480
Gene position: 5039830-5042262 (Clockwise)
Preceding gene: 209398697
Following gene: 209401006
Centisome position: 90.45
GC content: 58.57
Gene sequence:
>2433_bases ATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTG TTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCTGGTAGCACCA CTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGC TATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCCGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGATCTTGA GCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGCGAAGTGTGGTCGG CGCGCCTGATGTCTGCGGTGCTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAA CGCGCTGCGCAACCGCAGGTTGATGAAGGGCTTTCTTACCCGTTGCTGCAACAGCTACTGGTACAACATCCGGGTAAACG TCTGGTGGTGACCGGGTTTATCAGCCGTAACAACGCCGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCG CGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGTGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGATCCG CGTAAAGTGAAAGATGCCTGTCTGCTGCCATTGCTGCGTCTGGATGAAGCCAGCGAACTGGCGCGTCTGGCGGCTCCCGT TCTTCACGCCCGTACTTTACAGCCAGTATCCGGTAGCGAAATCGACCTGCAGCTGCGCTGTAGCTACACGCCGGATCAAG GCTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGCTTGATT GAGTTTCAGGTGCCCACCAGCCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAGATCCTGAAACGTGCTCAGGTGCG CCCACTGGCGGTTGGCGTGCATAACGATCGCCAGTTGCTGCAATTTTGCTACACCTCAGAAGTGGCCGACAGTGCGCTGA AAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGGGCGGGT GTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAGTTTACCTGGCAGTCCGA TGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGCCTGCATCAGTCCGTCTTCC GCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGCAAAGGCAATATCGGTTCCCGCTGGCTGGAACTGTTCGCCCGCGAG CAGAGCACGCTTTCGGCGCGTACCGGCTTTGAATTTGTGCTGGCAGGCGTGGTGGACAGCCGCCGCAGTCTGTTGAGTTA TGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGT GGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTGGCTGACCAGTATCTTGAT TTCGCCAGCCACGGTTTCCACGTCATCAGTGCCAACAAACTGGCGGGAGCAAGCGACAGCAATAAATATCGCCAGATCCA CGACGCCTTCGAAAAAACCGGGCGTCACTGGCTATACAACGCCACCGTCGGTGCGGGCTTGCCGATCAACCATACCGTGC GCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGTGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAA TTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAGCCTGATCCGCGTGACGA CCTCTCCGGCAAAGACGTAATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCGGGTTACAACATTGAACCGGACCAGGTAC GTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTTAACGAG CAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGCAA AGCGCGTGTGGGCGTGGAGGCGGTGCGTGAAGACCATCCGCTGGCATCACTGCTACCGTGCGATAACGTCTTTGCCATCG AAAGCCGCTGGTATCGCGACAACCCGCTGGTGATCCGTGGGCCTGGCGCGGGGCGCGACGTCACCGCCGGGGCGATTCAG TCGGATATCAACCGGCTGGCACAGTTGTTGTAA
Upstream 100 bases:
>100_bases TCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGG GCTGCAAACAAGGGGTAAAA
Downstream 100 bases:
>100_bases TTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGAT TAGGCCGGATGAAGCGTTTA
Product: bifunctional aspartate kinase II/homoserine dehydrogenase II
Products: NA
Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]
Number of amino acids: Translated: 810; Mature: 809
Protein sequence:
>810_residues MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRR YQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLI EFQVPTSQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAG VTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLD FASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ FDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ SDINRLAQLL
Sequences:
>Translated_810_residues MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRR YQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLI EFQVPTSQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAG VTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLD FASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ FDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ SDINRLAQLL >Mature_809_residues SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRY QCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAER AAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPR KVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIE FQVPTSQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGV TRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFAREQ STLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDF ASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQF DGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQ MVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQS DINRLAQLL
Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]
COG id: COG0527
COG function: function code E; Aspartokinases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]
Homologues:
Organism=Escherichia coli, GI1790376, Length=810, Percent_Identity=99.8765432098765, Blast_Score=1647, Evalue=0.0, Organism=Escherichia coli, GI1786183, Length=821, Percent_Identity=30.694275274056, Blast_Score=333, Evalue=2e-92, Organism=Escherichia coli, GI1790455, Length=288, Percent_Identity=31.25, Blast_Score=112, Evalue=1e-25, Organism=Saccharomyces cerevisiae, GI6322599, Length=370, Percent_Identity=35.1351351351351, Blast_Score=175, Evalue=3e-44, Organism=Saccharomyces cerevisiae, GI6320893, Length=491, Percent_Identity=23.2179226069246, Blast_Score=79, Evalue=4e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001048 - InterPro: IPR005106 - InterPro: IPR001341 - InterPro: IPR018042 - InterPro: IPR011147 - InterPro: IPR001342 - InterPro: IPR019811 - InterPro: IPR016040 [H]
Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]
EC number: =2.7.2.4; =1.1.1.3 [H]
Molecular weight: Translated: 88919; Mature: 88788
Theoretical pI: Translated: 5.27; Mature: 5.27
Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW CCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCHHEEHHHHHHHHCCCCC RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH VLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCEEEEEECCCC DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCHHHHCCCHHHHH QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD HHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHCCCCCHHHCCCCCEEEECCCEECC NPLVIRGPGAGRDVTAGAIQSDINRLAQLL CCEEEECCCCCCCCHHHHHHHHHHHHHHHC >Mature Secondary Structure SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW CCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCHHEEHHHHHHHHCCCCC RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH VLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCEEEEEECCCC DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCHHHHCCCHHHHH QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD HHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHCCCCCHHHCCCCCEEEECCCEECC NPLVIRGPGAGRDVTAGAIQSDINRLAQLL CCEEEECCCCCCCCHHHHHHHHHHHHHHHC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 6298218; 8346018; 9278503 [H]