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The map label for this gene is dnaC

Identifier: 209399469

GI number: 209399469

Start: 5532220

End: 5532957

Strand: Reverse

Name: dnaC

Synonym: ECH74115_5874

Alternate gene names: 209399469

Gene position: 5532957-5532220 (Counterclockwise)

Preceding gene: 209398718

Following gene: 209397906

Centisome position: 99.3

GC content: 50.95

Gene sequence:

>738_bases
ATGAAAAACGTTGGCGACCTGATGCAACGCCTGCAAAAAATGATGCCTGCCCATATCAAACCTGCATTCAAAACGGGTGA
AGAGCTACTGGCCTGGCAGAAAGAACAAGGGGCGATCCGCTCCGCCGCTCTCGAACGTGAAAATCGGGCGATGAAAATGC
AGCGCACCTTTAACCGCTCCGGTATTCGCCCACTGCATCAGAACTGCTCCTTTGAGAACTATCGCGTTGAGTGTGAAGGG
CAGATGAATGCGTTAAGCAAAGCGCGCCAGTATGTCGAAGAGTTTGACGGCAACATCGCCAGCTTCATCTTTTCTGGTAA
GCCAGGAACCGGCAAAAACCATCTGGCGGCGGCAATCTGCAACGAGCTGCTGCTACGCGGTAAATCCGTATTGATCATCA
CCGTGGCCGATATTATGTCGGCGATGAAAGATACCTTCAGGAATAGCGGTACCAGCGAAGAACAACTGCTTAACGATCTG
AGCAACGTTGATCTGCTGGTGATCGATGAGATCGGCGTGCAGACCGAATCGAAATACGAAAAAGTGATCATCAACCAGAT
CGTCGATCGCCGCTCTTCTTCCAAACGCCCAACCGGGATGCTGACCAACAGTAATATGGAAGAGATGACCAAGTTGCTGG
GCGAACGCGTGATGGACCGTATGCGCCTGGGTAACAGTTTGTGGGTGATCTTCAACTGGGATAGCTACCGTAGCCGTGTA
ACAGGTAAAGAGTATTAA

Upstream 100 bases:

>100_bases
TGCAAATCGGTCGTGCCAGCAACGGCGGACTGCCGAAACGAGATGTGAATACGGTCAGTGAACCTGACAGCCAAATTCCA
CCAGGATTCAGAGGGTAACG

Downstream 100 bases:

>100_bases
TCCCCGTTTCTGGCGGTATACTGCGACACAATTCCACCTTGAGTAATGATGATGAAAACTGTTAAACATCTTCTGTGCTG
TGCCATTGCCGCCAGCGCAT

Product: DNA replication protein DnaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAALERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEG
QMNALSKARQYVEEFDGNIASFIFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVMDRMRLGNSLWVIFNWDSYRSRV
TGKEY

Sequences:

>Translated_245_residues
MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAALERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEG
QMNALSKARQYVEEFDGNIASFIFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVMDRMRLGNSLWVIFNWDSYRSRV
TGKEY
>Mature_245_residues
MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAALERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEG
QMNALSKARQYVEEFDGNIASFIFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVMDRMRLGNSLWVIFNWDSYRSRV
TGKEY

Specific function: This protein is required for chromosomal replication. It forms, in concert with dnaB protein and other prepriming proteins dnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaC family

Homologues:

Organism=Escherichia coli, GI1790823, Length=245, Percent_Identity=100, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1787623, Length=243, Percent_Identity=50.2057613168724, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAC_ECO57 (P0AEF2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91294
- PIR:   D86135
- RefSeq:   NP_290977.1
- RefSeq:   NP_313348.1
- ProteinModelPortal:   P0AEF2
- SMR:   P0AEF2
- EnsemblBacteria:   EBESCT00000026324
- EnsemblBacteria:   EBESCT00000059910
- GeneID:   913577
- GeneID:   959692
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5961
- KEGG:   ecs:ECs5321
- GeneTree:   EBGT00050000009124
- HOGENOM:   HBG575938
- OMA:   VIFNWES
- ProtClustDB:   PRK07952
- BioCyc:   ECOL83334:ECS5321-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR002611
- SMART:   SM00382

Pfam domain/function: PF01695 IstB

EC number: NA

Molecular weight: Translated: 27936; Mature: 27936

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAALERENRAMKMQRTFNRS
CCCHHHHHHHHHHHCCHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASFIFSGKPGTGKNHLAAAIC
CCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
NELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDLSNVDLLVIDEIGVQTESKYE
HHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHH
KVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVMDRMRLGNSLWVIFNWDSYRSRV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCC
TGKEY
CCCCC
>Mature Secondary Structure
MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAALERENRAMKMQRTFNRS
CCCHHHHHHHHHHHCCHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASFIFSGKPGTGKNHLAAAIC
CCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
NELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDLSNVDLLVIDEIGVQTESKYE
HHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHH
KVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVMDRMRLGNSLWVIFNWDSYRSRV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCC
TGKEY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796