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The map label for this gene is ydeH [H]

Identifier: 209399358

GI number: 209399358

Start: 2063455

End: 2064345

Strand: Reverse

Name: ydeH [H]

Synonym: ECH74115_2151

Alternate gene names: 209399358

Gene position: 2064345-2063455 (Counterclockwise)

Preceding gene: 209397734

Following gene: 209400506

Centisome position: 37.05

GC content: 44.44

Gene sequence:

>891_bases
ATGATCAAGAAGACAACGGAAATTGATGCCATCTTGTTAAATCTCAATAAGGCTATCGATGCCCACTATCAGTGGCTGGT
GAGTATGTTTCACAGCGTGGTCGCGAGAGATGCCAGTAAGCCAGAAATAACGGATAACCATTCTTATGGACTGTGCCAGT
TTGGTCGGTGGATTGATCATCTGAGGCCACTCGATAACGATGAATTACCTTACGTTCGGCTAATGGATTCTGCCCATCAA
CATATGCATAATTGTGGTCGGGAATTAATGCTGGCTATTGTTGAAAATCACTGGCAGGACGCGCATTTCGACGCCTTTCA
GGAGGGGGTGCTTTCTTTTACTGCGGCATTAACCGATTACAAAATTTATTTGCTGACGATCCGTAGCAATATGGATGTTT
TGACGGGATTGCCGGGGCGTCGGGTTCTTGATGAATCCTTTGATCATCAGTTACGCAACACTGAGCCTCTGAATCTTTAT
TTAATGTTGTTGGATATTGACCGATTTAAATTGGTTAATGATACCTACGGGCATTTAATCGGCGATGTAGTATTACGCAC
CCTGGCAACTTACTTAGCCAGTTGGACGCGTGATTACGAAACGGTTTATCGCTACGGGGGCGAAGAATTTATCATTATTG
TCAAAGCGGCTAATGATGAAGAAGCATGTCGTGCAGGTGTCAGAATTTGCCAGTTAGTCGATAACCATGCCATCACACAT
TCTGAAGGGCATATCAACATTACCGTGACAGCAGGTGTGAGTCGCGCATTTCCTGAAGAGACTCTGGATGTGGTCATTGG
AAGAGCAGACCGGGCAATGTATGAGGGTAAGCAAACCGGAAGAAATCGCTGCATGTTTATTGACGAACAAAATGTGATTA
ACCGAGTTTAA

Upstream 100 bases:

>100_bases
AGATGAATCATAACTTCGGATTTCTATGAAAATATTGACGTAATTTTGCTTTTCTCTCGTTAGAATAGCGCGCACAAGGA
ACTGTGAAAAAGGAGTGGCA

Downstream 100 bases:

>100_bases
GCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATCCGTTATCAGTGCAGGAAAATGCCTGTTAGCG
TAAAAGCAAAACACAAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Sequences:

>Translated_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
>Mature_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=296, Percent_Identity=98.6486486486486, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=34.1614906832298, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787056, Length=191, Percent_Identity=32.9842931937173, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=32.1637426900585, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=36.1963190184049, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=31.1111111111111, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=29.8780487804878, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=33.1428571428571, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=30.6748466257669, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=34.7560975609756, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=31.4814814814815, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=151, Percent_Identity=29.1390728476821, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=25.5952380952381, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33983; Mature: 33983

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGVLSFTAALTDY
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHCHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
SEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHCCHHCCCCCEEEEECHHHHCCC
>Mature Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGVLSFTAALTDY
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHCHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
SEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHCCHHCCCCCEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]