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The map label for this gene is rumB [H]

Identifier: 209399215

GI number: 209399215

Start: 1027168

End: 1028295

Strand: Direct

Name: rumB [H]

Synonym: ECH74115_1015

Alternate gene names: 209399215

Gene position: 1027168-1028295 (Clockwise)

Preceding gene: 209397452

Following gene: 209398665

Centisome position: 18.43

GC content: 54.88

Gene sequence:

>1128_bases
ATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAACGCAGCCGATTCCAGAGCAACTCTCCGC
TAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAG
GGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCTCGGTATGCTGCATCGCGACGGCACA
CCGGAAGACCTTTGTGACTGCCCGCTTTATCCTGCCTCATTTGCGCCCGTTTTTGCGGCGCTAAAACCGTTTATCGCCCG
AGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGCGATG
GCGGCATGATGCTGCGCTTTGTACTGCGTTCTGAAACCAAACTAGCACAACTGCGTAAGGCATTGCCGTGGTTACAGGCA
CAATTGCCGCAGCTAAAAGTTATCACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTA
CCTGACCGAACAACAGGCGCTGGTAGAGCGTTTTAACGATGTGCCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTA
ATCCGGCGGTCGCCAGCCAGCTGTACGCTACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTG
TTCTGCGGTGTGGGGGGCTTTGGTTTACACTGCGCGACGCCTGACATGCAGTTAACCGGGATCGAAATTGCGCTAGAGGC
CATTGCCTGTGCAAAGCAGTCAGCCGCTGAACTGGGCTTAACGCGTCTGCAATTTCAGGCGCTGGACTCCACTCAGTTTG
CCACCGCTCAGGGGGAGGTGCCGGAGCTGGTGCTGGTTAACCCGCCGCGCCGCGGCATTGGTAAACCGCTGTGTGATTAT
CTCTCAACGATGGCACCGCGTTTTATTATCTACTCCAGCTGTAACGCCCAAACCATGGCGAAAGATATCCGCGAACTGCC
AGGTTACCGCATCGAACGGGTACAGCTTTTTGATATGTTCCCGCACACCGCGCACTATGAAGTGCTGACGCTGCTGGTAA
AAATGTAA

Upstream 100 bases:

>100_bases
CTCATCCTGATGCTGCTGTTCGTTCCCTCGACCAGTCGGCGGTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCT
GATGATGTGAAGGTCAATGT

Downstream 100 bases:

>100_bases
ATACATCATTCAAATCAATATCACCTCGGCATACATCCATCCAAAATAATAATCGTGATCGATCGATTAATAATAACCGA
TCGATCACGGTTATATATTG

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM

Sequences:

>Translated_375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM
>Mature_375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=192, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI51173878, Length=192, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787083, Length=374, Percent_Identity=97.8609625668449, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=384, Percent_Identity=27.0833333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=72, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24666579, Length=358, Percent_Identity=24.0223463687151, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41960; Mature: 41960

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQAQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEECHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCC
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMF
CEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
PHTAHYEVLTLLVKM
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQAQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEECHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCC
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMF
CEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
PHTAHYEVLTLLVKM
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA