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The map label for this gene is hcaD [H]

Identifier: 209399212

GI number: 209399212

Start: 3496665

End: 3497867

Strand: Direct

Name: hcaD [H]

Synonym: ECH74115_3774

Alternate gene names: 209399212

Gene position: 3496665-3497867 (Clockwise)

Preceding gene: 209397724

Following gene: 209395795

Centisome position: 62.75

GC content: 54.11

Gene sequence:

>1203_bases
ATGAAAGAAAAAACGATCATTATTGTCGGGGGCGGGCAAGCGGCGGCAATGGCTGCGGCCTCGCTACGCCAGCAAGGGTT
CACCGGTGAGCTGCATCTGTTTTCCGATGAGCAACATCTTCCTTATGAACGCCCTCCGCTCTCGAAATCCATGTTGCTGG
AAGATTCCCCACAGTTGCAGTCTGTGTTACCCGCTCACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACC
ATCAAAACATTGGGCCGCGACACACGAGAGTTAGTGTTAGCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGC
AACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTGCGCCATGCCGGTGATG
CCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTGTCGGTGCCGGAACTATTGGTCTGGAACTGGCT
GCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCC
CGTGCAACGCTATCTTTTACAGCGCCATCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCG
ATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGCGAGACGCTTCAGGCCGATGTGGTGATTTACGGTATTGGTATCAGC
GCCAACGACCAACTGGCTCGCGAGGCCAACCTTGATACTACCAATGGCATTGTCATTGATGAGGCTTGCCGCACCTGCGA
TCCCGCGATCTTTGCCGGTGGCGATGTGGCAATCACTCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAA
ACGCCAATAACCATGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTTCCGCTACTGCCGCCGCCGTGGTTCTGG
AGTGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAAC
CCAGAAAGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTAACGCTGAATCAGGGACGTGAGATTCGCT
CAATCCGCAAATGGATCCAGAGCGGCAAAACGTTTGATGCGAAACAGCTGACAGATGAGAACATCGCGCTTAAATCACTG
TAA

Upstream 100 bases:

>100_bases
TAATCGCGCATTAAGCGGTGTGATGATCAACGCTGATGCGGGTTTAGCAATTCGCGGCATTCGCCACGTAGCGGCTGGGT
TGGATCTTTAAGGAAGTACG

Downstream 100 bases:

>100_bases
CCAGGATAATTAGCGAATATCTCAACGCCTGGGGCGTGGCGAGGTACCAGAGTGTGTATTACGTTTAAATCACATTATCT
TGCAAAGGGATTGGTTATGA

Product: phenylpropionate dioxygenase ferredoxin reductase subunit

Products: NA

Alternate protein names: Digoxigenin system ferredoxin--NAD(+) reductase component [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVT
IKTLGRDTRELVLANGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTDENIALKSL

Sequences:

>Translated_400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVT
IKTLGRDTRELVLANGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTDENIALKSL
>Mature_400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVT
IKTLGRDTRELVLANGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTDENIALKSL

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP- dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=400, Percent_Identity=27, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21389617, Length=400, Percent_Identity=27, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI65787454, Length=400, Percent_Identity=27, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=24.9258160237389, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=24.9258160237389, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=97.25, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=274, Percent_Identity=28.4671532846715, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789765, Length=323, Percent_Identity=24.1486068111455, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=372, Percent_Identity=27.1505376344086, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639257, Length=399, Percent_Identity=27.3182957393484, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281359715, Length=399, Percent_Identity=27.3182957393484, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281359713, Length=399, Percent_Identity=27.3182957393484, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639250, Length=399, Percent_Identity=27.3182957393484, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI18543267, Length=399, Percent_Identity=27.3182957393484, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639252, Length=399, Percent_Identity=27.3182957393484, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24585130, Length=399, Percent_Identity=22.5563909774436, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24581020, Length=363, Percent_Identity=25.8953168044077, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573993, Length=363, Percent_Identity=25.8953168044077, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 43907; Mature: 43907

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQ
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCCHHH
SVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWDQLFIATGAAARPLPLLDA
HHCCHHHCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHH
LGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELAASATQRRCKVTVIELAATVM
HHHHHEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHH
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
CCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEECCC
ANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNHAQ
CCCHHHHHCCCCCCCCEEEECHHCCCCCCEEECCCEEEEEECCCHHHHHHCCCCCCCCHH
IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEEEECCCEE
IGAVTLNQGREIRSIRKWIQSGKTFDAKQLTDENIALKSL
EEEEEECCCHHHHHHHHHHHCCCCCCHHHCCCCCEEEECC
>Mature Secondary Structure
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQ
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCCHHH
SVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWDQLFIATGAAARPLPLLDA
HHCCHHHCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHH
LGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELAASATQRRCKVTVIELAATVM
HHHHHEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHH
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
CCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEECCC
ANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNHAQ
CCCHHHHHCCCCCCCCEEEECHHCCCCCCEEECCCEEEEEECCCHHHHHHCCCCCCCCHH
IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEEEECCCEE
IGAVTLNQGREIRSIRKWIQSGKTFDAKQLTDENIALKSL
EEEEEECCCHHHHHHHHHHHCCCCCCHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA