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The map label for this gene is purB [H]

Identifier: 209399204

GI number: 209399204

Start: 1549016

End: 1550386

Strand: Reverse

Name: purB [H]

Synonym: ECH74115_1592

Alternate gene names: 209399204

Gene position: 1550386-1549016 (Counterclockwise)

Preceding gene: 209398608

Following gene: 209395806

Centisome position: 27.82

GC content: 52.59

Gene sequence:

>1371_bases
ATGGAATTATCCTCACTGACCGCCGTTTCCCCTGTCGATGGACGCTACGGCGATAAAGTCAGCGCGCTGCGCGGGATTTT
CAGCGAATATGGTTTGCTGAAATTCCGTGTACAAGTTGAAGTACGTTGGCTGCAAAAACTGGCCGCGCACGCAGCGATCA
AGGAAGTTCCTGCTTTTGCTGCCGACGCAATCGGTTACCTTGATGCAATTGTCGCCAATTTCAGCGAAGAAGATGCCGCA
CGCATCAAAACCATCGAGCGTACTACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGAGAT
CCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAA
TGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCGTACTGGCGTCAACTAATTGATGGCATTAAAGATCTCGCCGTTCAG
TATCGCGATATCCCGCTGCTGTCCCGTACCCACGGTCAGCCAGCCACGCCGTCAACCATCGGTAAAGAGATGGCAAACGT
CGCCTACCGTATGGAGCGCCAGTACCGCCAGCTTAACCAGGTGGAGATCCTCGGCAAAATCAACGGCGCGGTCGGTAACT
ATAACGCCCACATCGCCGCTTACCCGGAAGTTGACTGGCATCAGTTCAGCGAAGAGTTCGTCACCTCGCTGGGTATTCAA
TGGAACCCGTACACTACCCAGATTGAACCGCACGACTACATTGCCGAACTGTTTGATTGCGTTGCGCGCTTCAACACCAT
TCTGATCGACTTTGACCGTGACGTCTGGGGTTATATCGCCCTTAACCACTTCAAACAGAAAACCATTGCTGGTGAGATTG
GTTCTTCCACCATGCCGCATAAAGTTAACCCGATCGACTTCGAAAACTCCGAAGGGAATCTGGGCCTTTCCAACGCGGTA
TTGCAGCATCTGGCAAGCAAACTGCCGGTTTCCCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGTAACCTCGG
CGTGGGTATCGGTTATGCGCTGATTGCGTATCAATCCACCCTGAAAGGCGTGAGCAAACTGGAAGTGAACCGTGACCATC
TGCTGGATGAGCTGGATCACAACTGGGAAGTGCTGGCAGAACCAATCCAGACAGTTATGCGTCGCTATGGCATCGAAAAA
CCGTACGAGAAGCTGAAAGAGCTGACTCGCGGTAAGCGCGTTGACGCCGAAGGCATGAAGCAGTTTATCGACGGTCTGGC
GTTGCCAGAAGAAGAGAAAGCCCGCCTGAAAGCGATGACGCCGGCTAACTACATTGGTCGCGCCATCACCATGGTTGATG
AGCTGAAATAA

Upstream 100 bases:

>100_bases
CCAGGTCGGCGGCGGACGTCTGCAACTGATGTTTTCTCGTAATCGCCTGACCACTCAGGCAAAACAAATTCTTGCTCATT
TAACCCCGGAGTTGTGATCT

Downstream 100 bases:

>100_bases
ACTCGTACCAGTGCCGGATGGCGATGCTGTCCGGCCTGCTTATTAAGATTATCCGCTTTTTATTTTTTCACTTTACCTCC
CCTCCCCCGCTGGTTTATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK

Sequences:

>Translated_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
>Mature_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=99.780701754386, Blast_Score=943, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51570; Mature: 51570

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTI
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHH
GKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTI
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHH
GKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]