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The map label for this gene is yagT

Identifier: 209399171

GI number: 209399171

Start: 338172

End: 338861

Strand: Reverse

Name: yagT

Synonym: ECH74115_0332

Alternate gene names: 209399171

Gene position: 338861-338172 (Counterclockwise)

Preceding gene: 209397316

Following gene: 209398590

Centisome position: 6.08

GC content: 54.78

Gene sequence:

>690_bases
ATGAGCAACCAAGGCGAATACCCCGAAGATAATCGGGTTGGGAAGCATGAGCCGCACGATTTAAGTTTGACCCGTCGCGA
TCTGATTAAAGTGAGCGCCGCAACGGCGGCGGCCGCCGTGGTTTATCCTCATTCTACGCTTGCGGCAAGCGTTCCGGCAG
CTACACCCGCGCCTGAGATAATGCCCCTGACACTTAAGGTGAACGGCAAAACCGAGCAGCTTGAGGTGGATACCCGAACC
ACGCTACTGGACGCTTTGCGTGAAAATCTGCATTTGATCGGTACCAAGAAAGGTTGCGATCACGGACAGTGCGGAGCCTG
TACCGTGCTGGTCAATGGTCGCAGGCTTAATGCCTGCCTGACGCTTGCAGTCATGCATCAGGGGGCCGAGATCACCACCA
TTGAAGGCCTGGGTTCACCAGATAATCTTCACCCCATGCAGGCGGCCTTTATCAAGCATGATGGCTTCCAGTGCGGCTAC
TGCACCTCCGGGCAAATTTGCTCATCAGTAGCGGTGCTAAAAGAGATTCAGGACGGCATTCCCAGTCACGTCACGGTCGA
TTTGGTTTCCCCTCCAGAAAGAACTGCCGATGAGATCCGTGAACGTATGAGCGGCAACATCTGTCGCTGTGGTGCATACG
CTAACATCCTTGCCGCCATTGAAGACGCTGCGGGGGAGATAAAATCATGA

Upstream 100 bases:

>100_bases
TAATCCTCCTGCTGTCGCCGACTATGCTTAACGTTTAAAAAAGCATCAGCACTCTCGCAACGCACTCTTATTTTCCCCTT
TAGAATACCGGAGGCCTGGT

Downstream 100 bases:

>100_bases
AGGCGTTTACCTATGAACGAGTGAACACCCCCGCCGAGGCGGCACTTAGCGCTCAGCGCGTACCCGGCGCAAAATTTATC
GCGGGCGGGACCAATCTGCT

Product: putative xanthine dehydrogenase iron-sulfur-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRT
TLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS

Sequences:

>Translated_229_residues
MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRT
TLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS
>Mature_228_residues
SNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTT
LLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYC
TSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS

Specific function: Iron-sulfur subunit of the xanthine dehydrogenase complex

COG id: COG2080

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain

Homologues:

Organism=Homo sapiens, GI91823271, Length=175, Percent_Identity=38.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI71773480, Length=169, Percent_Identity=37.8698224852071, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786480, Length=229, Percent_Identity=98.2532751091703, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1789232, Length=166, Percent_Identity=36.7469879518072, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540638, Length=181, Percent_Identity=36.4640883977901, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32566215, Length=180, Percent_Identity=33.8888888888889, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17539860, Length=169, Percent_Identity=33.1360946745562, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17737937, Length=177, Percent_Identity=37.8531073446328, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647197, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24647195, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24647199, Length=149, Percent_Identity=33.5570469798658, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647193, Length=174, Percent_Identity=30.4597701149425, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647201, Length=172, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YAGT_ECO57 (Q8X6I9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90668
- PIR:   G85518
- RefSeq:   NP_286003.1
- RefSeq:   NP_308343.1
- ProteinModelPortal:   Q8X6I9
- SMR:   Q8X6I9
- EnsemblBacteria:   EBESCT00000025711
- EnsemblBacteria:   EBESCT00000058433
- GeneID:   914415
- GeneID:   957140
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0352
- KEGG:   ecs:ECs0316
- GeneTree:   EBGT00050000009268
- HOGENOM:   HBG292380
- OMA:   HEGQRIT
- ProtClustDB:   PRK11433
- BioCyc:   ECOL83334:ECS0316-MONOMER
- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006311
- Gene3D:   G3DSA:1.10.150.120
- Gene3D:   G3DSA:3.10.20.30
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2; SSF47741 2Fe-2S_bind; SSF54292 Ferredoxin

EC number: NA

Molecular weight: Translated: 24364; Mature: 24233

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPHSTLAASVPAATPAPEI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCCCCCCE
MPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACL
EEEEEEECCCCEEEEEHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEECCCCHHHHH
TLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYCTSGQICSSVAVLKEIQDGI
EEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCC
PSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS
CCCEEEEEECCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPHSTLAASVPAATPAPEI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCCCCCCE
MPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACL
EEEEEEECCCCEEEEEHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEECCCCHHHHH
TLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYCTSGQICSSVAVLKEIQDGI
EEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCC
PSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS
CCCEEEEEECCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796