| Definition | Escherichia coli O157:H7 str. EC4115, complete genome. |
|---|---|
| Accession | NC_011353 |
| Length | 5,572,075 |
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The map label for this gene is nikD
Identifier: 209399073
GI number: 209399073
Start: 4454697
End: 4455461
Strand: Direct
Name: nikD
Synonym: ECH74115_4819
Alternate gene names: 209399073
Gene position: 4454697-4455461 (Clockwise)
Preceding gene: 209400711
Following gene: 209399511
Centisome position: 79.95
GC content: 60.0
Gene sequence:
>765_bases ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTGTCGTTAACCCTGAA ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGAATTCTGC CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAGACGTGCCT GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTTTGCGAATCACCG TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCACGCATTCTCGATCTGCTGGAAAGCATTAT GCAAAAACAGGCGCCGGGGATGCTGCTGGTCACCCATGATATGGGCGTGGTGGCGCGTCTGGCGGATGACGTAGCGGTAA TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA
Upstream 100 bases:
>100_bases CGGGGCTGGCGCTGTTTATCAGCGTGATGGCCTTTAACCTGGTGGGTGACGCACTGCGCGATCATCTGGATCCTCATCTG GTGACGGAGCACGCACACTA
Downstream 100 bases:
>100_bases CTTTACTTAACGTCTCCGACCTTTCCCATCACTATGCCCACGGTGGGTTTAGCGGAAAACATCAGCACCAGGCGGTGCTG AATAACGTTTCCCTGACCCT
Product: nickel transporter ATP-binding protein NikD
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 254; Mature: 253
Protein sequence:
>254_residues MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL VSAHLALYGMELAS
Sequences:
>Translated_254_residues MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL VSAHLALYGMELAS >Mature_253_residues PQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKI ATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESPF IIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSLV SAHLALYGMELAS
Specific function: Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system
COG id: COG0444
COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component
Gene ontology:
Cell location: Cell inner membrane; Peripheral membrane protein
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ABC transporter domain
Homologues:
Organism=Homo sapiens, GI171184400, Length=262, Percent_Identity=28.6259541984733, Blast_Score=71, Evalue=8e-13, Organism=Homo sapiens, GI27436953, Length=212, Percent_Identity=25.4716981132075, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI153792144, Length=215, Percent_Identity=25.1162790697674, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI42490749, Length=230, Percent_Identity=27.3913043478261, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI116734710, Length=202, Percent_Identity=25.7425742574257, Blast_Score=68, Evalue=8e-12, Organism=Homo sapiens, GI105990541, Length=211, Percent_Identity=24.6445497630332, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI21536376, Length=208, Percent_Identity=27.8846153846154, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI27477115, Length=210, Percent_Identity=24.7619047619048, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI156105685, Length=223, Percent_Identity=30.9417040358744, Blast_Score=65, Evalue=6e-11, Organism=Homo sapiens, GI6005701, Length=210, Percent_Identity=24.7619047619048, Blast_Score=65, Evalue=8e-11, Organism=Escherichia coli, GI1789890, Length=254, Percent_Identity=99.2125984251969, Blast_Score=508, Evalue=1e-145, Organism=Escherichia coli, GI1788506, Length=241, Percent_Identity=41.0788381742739, Blast_Score=164, Evalue=6e-42, Organism=Escherichia coli, GI145693107, Length=257, Percent_Identity=38.5214007782101, Blast_Score=158, Evalue=4e-40, Organism=Escherichia coli, GI1789963, Length=234, Percent_Identity=36.7521367521368, Blast_Score=150, Evalue=1e-37, Organism=Escherichia coli, GI1787759, Length=251, Percent_Identity=33.8645418326693, Blast_Score=139, Evalue=1e-34, Organism=Escherichia coli, GI48994883, Length=234, Percent_Identity=35.4700854700855, Blast_Score=137, Evalue=5e-34, Organism=Escherichia coli, GI1789962, Length=232, Percent_Identity=35.7758620689655, Blast_Score=124, Evalue=5e-30, Organism=Escherichia coli, GI1787500, Length=232, Percent_Identity=32.7586206896552, Blast_Score=115, Evalue=2e-27, Organism=Escherichia coli, GI1787548, Length=241, Percent_Identity=29.045643153527, Blast_Score=107, Evalue=9e-25, Organism=Escherichia coli, GI1787758, Length=227, Percent_Identity=31.2775330396476, Blast_Score=107, Evalue=9e-25, Organism=Escherichia coli, GI1789891, Length=216, Percent_Identity=36.5740740740741, Blast_Score=106, Evalue=2e-24, Organism=Escherichia coli, GI1787547, Length=247, Percent_Identity=28.7449392712551, Blast_Score=103, Evalue=8e-24, Organism=Escherichia coli, GI48994997, Length=229, Percent_Identity=31.4410480349345, Blast_Score=95, Evalue=3e-21, Organism=Escherichia coli, GI1788225, Length=257, Percent_Identity=31.1284046692607, Blast_Score=95, Evalue=3e-21, Organism=Escherichia coli, GI1786398, Length=237, Percent_Identity=29.957805907173, Blast_Score=94, Evalue=1e-20, Organism=Escherichia coli, GI1786345, Length=224, Percent_Identity=31.25, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1789032, Length=230, Percent_Identity=30, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI87081834, Length=216, Percent_Identity=31.9444444444444, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI1787112, Length=228, Percent_Identity=28.5087719298246, Blast_Score=84, Evalue=7e-18, Organism=Escherichia coli, GI1790162, Length=253, Percent_Identity=25.296442687747, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI87081791, Length=236, Percent_Identity=30.9322033898305, Blast_Score=80, Evalue=1e-16, Organism=Escherichia coli, GI1787029, Length=239, Percent_Identity=27.1966527196653, Blast_Score=78, Evalue=7e-16, Organism=Escherichia coli, GI1787089, Length=244, Percent_Identity=29.0983606557377, Blast_Score=77, Evalue=1e-15, Organism=Escherichia coli, GI1787712, Length=254, Percent_Identity=28.3464566929134, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI87081709, Length=231, Percent_Identity=26.4069264069264, Blast_Score=76, Evalue=3e-15, Organism=Escherichia coli, GI1790739, Length=220, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=3e-15, Organism=Escherichia coli, GI1788450, Length=247, Percent_Identity=28.7449392712551, Blast_Score=75, Evalue=3e-15, Organism=Escherichia coli, GI1789672, Length=246, Percent_Identity=28.4552845528455, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=29.3269230769231, Blast_Score=75, Evalue=5e-15, Organism=Escherichia coli, GI1786803, Length=230, Percent_Identity=28.695652173913, Blast_Score=74, Evalue=9e-15, Organism=Escherichia coli, GI1787143, Length=226, Percent_Identity=28.3185840707965, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI48994943, Length=229, Percent_Identity=26.2008733624454, Blast_Score=73, Evalue=1e-14, Organism=Escherichia coli, GI1786703, Length=212, Percent_Identity=31.6037735849057, Blast_Score=73, Evalue=2e-14, Organism=Escherichia coli, GI1788540, Length=227, Percent_Identity=30.8370044052863, Blast_Score=73, Evalue=2e-14, Organism=Escherichia coli, GI1788761, Length=229, Percent_Identity=31.0043668122271, Blast_Score=72, Evalue=2e-14, Organism=Escherichia coli, GI1789586, Length=246, Percent_Identity=27.6422764227642, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1787164, Length=193, Percent_Identity=33.160621761658, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1786253, Length=222, Percent_Identity=28.8288288288288, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1787370, Length=216, Percent_Identity=27.3148148148148, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1786872, Length=207, Percent_Identity=34.2995169082126, Blast_Score=72, Evalue=5e-14, Organism=Escherichia coli, GI1788644, Length=128, Percent_Identity=31.25, Blast_Score=65, Evalue=5e-12, Organism=Escherichia coli, GI1787105, Length=210, Percent_Identity=30.4761904761905, Blast_Score=65, Evalue=5e-12, Organism=Escherichia coli, GI1788165, Length=217, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=5e-12, Organism=Escherichia coli, GI1786319, Length=218, Percent_Identity=25.2293577981651, Blast_Score=64, Evalue=7e-12, Organism=Escherichia coli, GI1789991, Length=218, Percent_Identity=27.0642201834862, Blast_Score=64, Evalue=8e-12, Organism=Escherichia coli, GI1786654, Length=265, Percent_Identity=27.9245283018868, Blast_Score=64, Evalue=9e-12, Organism=Escherichia coli, GI1787792, Length=230, Percent_Identity=30.4347826086957, Blast_Score=62, Evalue=5e-11, Organism=Caenorhabditis elegans, GI193208177, Length=253, Percent_Identity=25.6916996047431, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI71983510, Length=221, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=4e-13, Organism=Caenorhabditis elegans, GI71983523, Length=221, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=4e-13, Organism=Caenorhabditis elegans, GI71983516, Length=221, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=4e-13, Organism=Caenorhabditis elegans, GI115533592, Length=241, Percent_Identity=27.8008298755187, Blast_Score=70, Evalue=9e-13, Organism=Caenorhabditis elegans, GI193211015, Length=231, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI193202349, Length=241, Percent_Identity=25.3112033195021, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17567265, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI193211017, Length=231, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=5e-12, Organism=Caenorhabditis elegans, GI17510237, Length=220, Percent_Identity=28.6363636363636, Blast_Score=68, Evalue=6e-12, Organism=Caenorhabditis elegans, GI115535101, Length=227, Percent_Identity=27.7533039647577, Blast_Score=68, Evalue=6e-12, Organism=Caenorhabditis elegans, GI71996809, Length=231, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=8e-12, Organism=Caenorhabditis elegans, GI17565938, Length=221, Percent_Identity=27.6018099547511, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17567269, Length=222, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=8e-11, Organism=Saccharomyces cerevisiae, GI6322640, Length=224, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=5e-12, Organism=Drosophila melanogaster, GI23397593, Length=238, Percent_Identity=28.9915966386555, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI221500365, Length=226, Percent_Identity=26.1061946902655, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI116007184, Length=226, Percent_Identity=26.1061946902655, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI24643674, Length=208, Percent_Identity=24.0384615384615, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI28574533, Length=242, Percent_Identity=26.8595041322314, Blast_Score=67, Evalue=1e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NIKD_ECO57 (Q8X5U1)
Other databases:
- EMBL: AE005174 - EMBL: BA000007 - PIR: B86018 - PIR: B91172 - RefSeq: NP_290045.1 - RefSeq: NP_312373.1 - ProteinModelPortal: Q8X5U1 - SMR: Q8X5U1 - EnsemblBacteria: EBESCT00000028407 - EnsemblBacteria: EBESCT00000055799 - GeneID: 915804 - GeneID: 961233 - GenomeReviews: AE005174_GR - GenomeReviews: BA000007_GR - KEGG: ece:Z4871 - KEGG: ecs:ECs4346 - GeneTree: EBGT00050000008231 - HOGENOM: HBG758042 - OMA: AVYPCAL - ProtClustDB: PRK10418 - BioCyc: ECOL83334:ECS4346-MONOMER - HAMAP: MF_01711 - InterPro: IPR003439 - InterPro: IPR017871 - InterPro: IPR015857 - InterPro: IPR003593 - InterPro: IPR014138 - SMART: SM00382 - TIGRFAMs: TIGR02770
Pfam domain/function: PF00005 ABC_tran
EC number: =3.6.3.24
Molecular weight: Translated: 26791; Mature: 26660
Theoretical pI: Translated: 6.62; Mature: 6.62
Prosite motif: PS00211 ABC_TRANSPORTER_1; PS50893 ABC_TRANSPORTER_2; PS51247 NIKD
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 5.1 %Met (Translated Protein) 6.7 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 4.7 %Met (Mature Protein) 6.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQT CCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCEEEEEEHHCCCCCCHHHH AGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTA HHHHHCCCCCCCHHHHCCEEEEEECCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHH AIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESPFIIADEPTTDLDVVAQARIL HHHHHCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH DLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL HHHHHHHHHCCCCEEEEEECCHHHHHHHHHHEEECCCCEEECCCHHHHHCCCHHHHHHHH VSAHLALYGMELAS HHHHHHHHHHHHCC >Mature Secondary Structure PQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQT CCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCEEEEEEHHCCCCCCHHHH AGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTA HHHHHCCCCCCCHHHHCCEEEEEECCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHH AIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESPFIIADEPTTDLDVVAQARIL HHHHHCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH DLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL HHHHHHHHHCCCCEEEEEECCHHHHHHHHHHEEECCCCEEECCCHHHHHCCCHHHHHHHH VSAHLALYGMELAS HHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 7.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796