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The map label for this gene is intB [H]

Identifier: 209398968

GI number: 209398968

Start: 3956630

End: 3957895

Strand: Direct

Name: intB [H]

Synonym: ECH74115_4272

Alternate gene names: 209398968

Gene position: 3956630-3957895 (Clockwise)

Preceding gene: 209398981

Following gene: 209399039

Centisome position: 71.01

GC content: 47.55

Gene sequence:

>1266_bases
ATGGCACTGACTGACGCAAAAATCCGGGCTGCAAAGCCCACTGACAAGGCTTATAAACTCACTGACGGGGCTGGCATGTT
CCTGCTGGTACATCCTAATGGTTCCCGTTACTGGCGTCTCCGTTATCGTATTCTGGGTAAAGAGAAGACTCTGGCACTTG
TTGTGTATCCAGAAGTATCTCTCTCCGAAGCTCGTACAAAACGGGATGAGGCCCGAAAACTGATTTCGGAGGGGGTTGAC
CCTTGCGAACAGAAAAGAGCTAAAAAAGTAGTCCCTGATTTACAACTCTCTTTCGAACATATTGCACGACGCTGGCATGC
CAGTAATAAACAATGGGCACAATCACACAGCGATAAAGTACTCAAAAGCCTCGAGACACACGTATTCCCCTTTATCGGCA
ATCGGGATATCACAACACTCAATACCCCGGACCTGCTTATCCCTGTTCGTGCTGCAGAAGCTAAACAAATTTATGAAATC
GCCAGTCGTCTGCAGCAAAGAATATCTGCCGTAATGCGTTATGCCGTACAGTCTGGCATCATCAGATATAATCCTGCTCT
GGATATGGCTGGCGCATTGACTACGGTAAAACGCCAGCATCGCCCCGCTCTTGATCTTTCTCGCCTGCCTGAACTTTTGT
CGCGTATTGGCAGTTACAAGGGGCAACCTGTCACCCAGCTTGCCGTTATGCTGAATTTACTGGTTTTTATTCGTTCCAGT
GAACTCAGATACGCCCGCTGGTCTGAAATTGATATTGACAATGCCATGTGGACTATTCCAGCCGAACGCGAACCTCTGCC
AGGCGTAAAATTCTCACACCGGGGATCCAAGATGCGAACACCACATCTTGTGCCACTCAGCAAACAGGTTGTAGCCATAC
TGGCAGAACTTCAGACATGGGCTGGTGAAAATGGTCTGATCTTTACGGGAGCACATGACCCACGTAAACCAATCAGTGAA
AACACCGTAAATAAAGCCCTGAGGGTTATGGGGTATGACACAACCCAGGATGTCTGTGGTCATGGATTCCGGGCGATGGC
ATGTAGTGCGTTAATAGAATCAGGTTTGTGGTCCCGCGATGCAGTTGAACGTCAGATGAGCCATCAGGAACGTAACGGTG
TACGTGCTGCTTACATTCATAAAGCAGAACATCTGGAAGAACGGCGACTGATGCTACAGTGGTGGGCCGATTTTCTGGAT
GCAAACAGAGAAAGATTTATCAGTCCATTTGAATATGCAAAGATTAATAATCCATTAAAACAGTAA

Upstream 100 bases:

>100_bases
AAATTCTCCCCACATCAACCTCATTGTGTGGGTATAATTGCGGGCATACCCCAGTTCGACAGAATTTGTACCCTCTTTAA
CGCATTCAGAAGGCTGAACT

Downstream 100 bases:

>100_bases
TCATCCCGGGCAAATGCCCGGGAACCTACTCCAGGATTATTTTCTTTGTTAAAAAAACAGACAAAACGGTATTAGCTTCG
TAAGCGTACAGCGAGGGCCG

Product: site-specific recombinase, phage integrase family protein

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVD
PCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIRSS
ELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISE
NTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLD
ANRERFISPFEYAKINNPLKQ

Sequences:

>Translated_421_residues
MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVD
PCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIRSS
ELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISE
NTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLD
ANRERFISPFEYAKINNPLKQ
>Mature_420_residues
ALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDP
CEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIA
SRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIRSSE
LRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISEN
TVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDA
NRERFISPFEYAKINNPLKQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=407, Percent_Identity=36.6093366093366, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788690, Length=404, Percent_Identity=34.9009900990099, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788974, Length=406, Percent_Identity=34.9753694581281, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48005; Mature: 47874

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALVVYPEVS
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEECCCCEEEEEEECCCC
LSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGI
HHHHHHHHCCEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIRSS
EEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
ELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTW
CCCEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRD
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCHH
AVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRERFISPFEYAKINNPLK
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCC
Q
C
>Mature Secondary Structure 
ALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALVVYPEVS
CCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEECCCCEEEEEEECCCC
LSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGI
HHHHHHHHCCEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIRSS
EEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
ELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTW
CCCEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRD
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCHH
AVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRERFISPFEYAKINNPLK
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]