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The map label for this gene is cynR

Identifier: 209398898

GI number: 209398898

Start: 418165

End: 419064

Strand: Reverse

Name: cynR

Synonym: ECH74115_0411

Alternate gene names: 209398898

Gene position: 419064-418165 (Counterclockwise)

Preceding gene: 209399698

Following gene: 209397539

Centisome position: 7.52

GC content: 55.33

Gene sequence:

>900_bases
ATGCTCTCTCGACATATCAATTATTTTCTTGCCGTGGCTGAACATGGCAGCTTCACCCGTGCCGCCAGTGCGTTGCACGT
CTCTCAGCCTGCGCTTTCCCAGCAGATTCGCCAGTTAGAGGAGAGTTTAGGCGTGCCGCTGTTTGACCGTAGCGGGCGAA
CGATTCATCTCACTGATGCAGGAGAAGTCTGGCGACAGTACGCCAGCCGGGCGTTACAGGAACTGGGAGCGGGTAAACGG
GCGATTCATGATGTTGCCGATCTGACGAGAGGATCGCTGCGTATCGCCGTCACCCCCACCTTTACGAGCTACTTTATCGG
CCCCTTAATGGCGGATTTCTATGCGCGCTACCCCGGCATCACGCTCCAGCTACAGGAAATGTCGCAGGAGAAAATCGAAG
ATCTGCTTTGTCGCGACGAGCTGGACGTTGGGATTGCCTTCGCGCCTGTGCATTTGCCGGAGCTGGAGGCAATTCCTTTA
CTGACAGAAAGTTTAGCGTTAGTCGTGGCGCAACATCATCCGCTGGCTGCCTGTGAACAGGTGGCGTTGAGTCGCTTGCA
TGATGAAAAACTGGTCCTGCTCAGCGCGGAATTTGCCACCAGAGAGCAGATTGACCACTACTGCGAGAAAGCGGGGCTGC
ATCCACAGGTGGTCATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTTCCACATTGTTA
CCAGCAGCGATTGCCACACAACATGACGGGCTTAAAGCGATTCCTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTT
GTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGCGAAGGCATTTTTGCACATGGCGTTGGAGGAATGTGCGGATGTTG
GCGAGAATGAATCGCGGTAA

Upstream 100 bases:

>100_bases
CTATCTGTCTCTGGAATGAGAGGCAGACTACTCGCCGATTGTCATAAGGTAAAAGTCTCATTTATGATGAGTTCTATTGA
ATTTACTTATAGGTTGGCGA

Downstream 100 bases:

>100_bases
CCACTGACACAATTGCCTGATGCGTTACGCTTATCAGGCCTACGAGTTCAGTGCTCTACGTAGGCCTGATAAGGCGTTCA
AGCCGCATCCGGCATAAACT

Product: DNA-binding transcriptional regulator CynR

Products: NA

Alternate protein names: Cyn operon transcriptional activator

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR

Sequences:

>Translated_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR
>Mature_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI157672245, Length=299, Percent_Identity=96.3210702341137, Blast_Score=579, Evalue=1e-167,
Organism=Escherichia coli, GI1790399, Length=276, Percent_Identity=30.7971014492754, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=28.4246575342466, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=29.6928327645051, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787530, Length=266, Percent_Identity=30.0751879699248, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=26.8965517241379, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081904, Length=259, Percent_Identity=31.2741312741313, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787601, Length=292, Percent_Identity=25.3424657534247, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788297, Length=243, Percent_Identity=29.6296296296296, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI2367136, Length=227, Percent_Identity=31.2775330396476, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=30.4498269896194, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789204, Length=230, Percent_Identity=28.2608695652174, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789173, Length=212, Percent_Identity=31.6037735849057, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786401, Length=171, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=182, Percent_Identity=31.8681318681319, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788296, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=24.5614035087719, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790262, Length=236, Percent_Identity=24.5762711864407, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789639, Length=142, Percent_Identity=33.8028169014084, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787949, Length=175, Percent_Identity=30.8571428571429, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786448, Length=158, Percent_Identity=34.1772151898734, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788481, Length=206, Percent_Identity=29.126213592233, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082132, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081684, Length=294, Percent_Identity=23.1292517006803, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082184, Length=257, Percent_Identity=24.9027237354086, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI145693193, Length=219, Percent_Identity=27.8538812785388, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786713, Length=175, Percent_Identity=29.1428571428571, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYNR_ECO57 (Q8X4M5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85528
- PIR:   G90677
- RefSeq:   NP_286079.1
- RefSeq:   NP_308418.1
- ProteinModelPortal:   Q8X4M5
- SMR:   Q8X4M5
- EnsemblBacteria:   EBESCT00000026355
- EnsemblBacteria:   EBESCT00000057046
- GeneID:   914493
- GeneID:   957263
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0434
- KEGG:   ecs:ECs0391
- GeneTree:   EBGT00050000008327
- HOGENOM:   HBG685425
- OMA:   IRCAREV
- ProtClustDB:   PRK11242
- BioCyc:   ECOL83334:ECS0391-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32971; Mature: 32971

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIHLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR
HHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIHLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR
HHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796