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The map label for this gene is galR [H]

Identifier: 209398804

GI number: 209398804

Start: 3803397

End: 3804428

Strand: Direct

Name: galR [H]

Synonym: ECH74115_4106

Alternate gene names: 209398804

Gene position: 3803397-3804428 (Clockwise)

Preceding gene: 209397783

Following gene: 209399771

Centisome position: 68.26

GC content: 52.81

Gene sequence:

>1032_bases
ATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAA
AGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC
TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCG
GTCGAACAGGTGGCTTATCACACCGGTAATTTCTTATTGATTGGCAATGGTTACCACAACGAACAAAAAGAGCGTCAGGC
CATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCCGATTTAGCCTCAT
TATTGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGAT
CGTTACGGTGCCTGGCTGGCTACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTC
TATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGG
TGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCG
GTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGAT
TTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGA
CGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGT
CCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAA

Upstream 100 bases:

>100_bases
TGGCTCACATTCCCACGATGAAAACACGCCGCCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAACAATGTAAGCGT
TTACCCACTAAGGTATTTTC

Downstream 100 bases:

>100_bases
CCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAA
ACAGAACTTCTGGTAACAGC

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKA
VEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFS
PTLVRRHSVSTPSLEASHHATSD

Sequences:

>Translated_343_residues
MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKA
VEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFS
PTLVRRHSVSTPSLEASHHATSD
>Mature_342_residues
ATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAV
EQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDDR
YGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTAV
ACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSP
TLVRRHSVSTPSLEASHHATSD

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=343, Percent_Identity=99.7084548104956, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=55.5891238670695, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=33.8278931750742, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790369, Length=304, Percent_Identity=33.2236842105263, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=32.6347305389222, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=32.1428571428571, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=28.3132530120482, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.5625, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786540, Length=355, Percent_Identity=27.0422535211268, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=25.077399380805, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=23.4527687296417, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=25.3164556962025, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786268, Length=128, Percent_Identity=32.03125, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37076; Mature: 36945

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTE
CCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALV
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEE
YLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
ELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASHHATSD
HHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTE
CCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALV
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEE
YLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
ELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASHHATSD
HHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]