Click here to switch to the map view.

The map label for this gene is guaD [H]

Identifier: 209398700

GI number: 209398700

Start: 3868316

End: 3869632

Strand: Direct

Name: guaD [H]

Synonym: ECH74115_4173

Alternate gene names: 209398700

Gene position: 3868316-3869632 (Clockwise)

Preceding gene: 209400599

Following gene: 209396772

Centisome position: 69.42

GC content: 48.97

Gene sequence:

>1317_bases
ATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCGCCCGTACGGTCGATAACCCGGAAGAGAT
TGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAG
ACGGAAAGCATCAAATTCCTGACACTATTCGCGTGCGCGACTATCGCGGCAAACTGATAGTACCGGGCTTTGTCGATACA
CATATCCATTATCCGCAAAGTGAAATGGTGGGGGCCTATGGGGAACAATTGCTGGAGTGGTTGAATAAACACACCTTCCC
TACTGAACGTCGTTATGAGGATTTAGAGTACGCCCGCGAAATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAA
CCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCCGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATG
CGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTAGACACTGCCGAAAGCAGCTATCACCA
AAGCAAAGAACTGATTGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCTACTTCTT
CTCCTGAACAGATGGCGATGGCGCAACGCCTGAAAGAGGAATATCCGGATACGTGGGTACATACCCATCTCTGTGAAAAC
AAAGATGAAATTGCCTGGGTGAAATCGCTTTATCCTGACCATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGAC
CGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCAAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCA
TTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGGTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAA
GTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTGTTGCA
ATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACG
ATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTG
CGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCAC
CTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAA

Upstream 100 bases:

>100_bases
TTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAT
TAACAGTAAAGGAGTCAATG

Downstream 100 bases:

>100_bases
TAATAAAACTTTTACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAG
GGCGCGCTGGATAATTATTT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVK
VGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQL
RYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Sequences:

>Translated_438_residues
MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVK
VGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQL
RYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN
>Mature_437_residues
SGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTH
IHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMR
MIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENK
DEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKV
GMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLR
YDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=381, Percent_Identity=36.745406824147, Blast_Score=229, Evalue=3e-60,
Organism=Escherichia coli, GI1789249, Length=438, Percent_Identity=99.0867579908676, Blast_Score=913, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319963, Length=418, Percent_Identity=33.4928229665072, Blast_Score=219, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24643849, Length=397, Percent_Identity=33.5012594458438, Blast_Score=205, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50070; Mature: 49938

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIP
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCC
DTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYARE
CCEEEEECCCCEEECCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDT
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEEHHHHEECCCCCCHHHHC
AESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
CHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLKEKEWDRLSETKSSIAFCPTS
CHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCC
NLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFY
CEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHEEE
LATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGD
EEECCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHHHEEECC
DRSIYRTYVDGRLVYERN
CCHHHHEEECCEEEEECC
>Mature Secondary Structure 
SGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWEDGKHQIP
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCC
DTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYARE
CCEEEEECCCCEEECCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDT
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEEHHHHEECCCCCCHHHHC
AESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
CHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLKEKEWDRLSETKSSIAFCPTS
CHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCC
NLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFY
CEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHEEE
LATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGD
EEECCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHHHEEECC
DRSIYRTYVDGRLVYERN
CCHHHHEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]