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The map label for this gene is dcuR

Identifier: 209398643

GI number: 209398643

Start: 5278965

End: 5279684

Strand: Reverse

Name: dcuR

Synonym: ECH74115_5638

Alternate gene names: 209398643

Gene position: 5279684-5278965 (Counterclockwise)

Preceding gene: 209397333

Following gene: 209398687

Centisome position: 94.75

GC content: 49.44

Gene sequence:

>720_bases
ATGATCAATGTATTAATTATCGATGACGACGCAATGGTCGCGGAGCTGAATCGCCGATACGTAGCACAAATCCCAGGCTT
TCAATGCTGTGGAACAGCCTCGACGCTGGAGAAAGCCAAAGAGATTATCTTCAATAGCGATACGCCTATCGACCTGATAT
TGCTCGATATCTATATGCAAAAAGAGAACGGGCTCGATTTACTGCCTGTCCTGCATAACGCGCGTTGCAAAAGTGATGTG
ATTGTCATCTCCTCCGCAGCCGATGCGGCAACCATTAAAGATTCGCTGCATTACGGTGTCGTGGATTACCTGATCAAACC
CTTCCAGGCTTCCCGCTTTGAAGAGGCGCTCACCGGCTGGCGGCAAAAGAAAATGGCGCTGGAAAAACATCAGTATTATG
ACCAGGCCGAGCTTGATCAGCTAATCCACGGCAGCAGCTCCAACGAGCAGGATCCTCGTCGCTTGCCAAAAGGCTTAACG
CCGCAGACGTTACGCACACTGTGCCAGTGGATTGACGCGCATCAGGACTATGAGTTTTCAACCGACGAGCTAGCCAACGA
GGTTAACATTTCGCGTGTTTCCTGCCGTAAATACCTTATCTGGCTGGTCAACTGCCACATCTTGTTCACCAGTATCCATT
ATGGCGTCACGGGGCGTCCAGTTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAA

Upstream 100 bases:

>100_bases
AGGTAGAAAATCTCGGCGGCAGCATCGCCGTGGAATCGGAACCCGGGATTTTCACACAATTTTTTGTCCAGATACCCTGG
GACGGGGAGAGGTCGAACAG

Downstream 100 bases:

>100_bases
CGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGTACAAGTTACATCCTTCATCAGTA
AAACTTGTGCCAGATCAAAT

Product: DNA-binding transcriptional activator DcuR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDV
IVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYCQ

Sequences:

>Translated_239_residues
MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDV
IVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYCQ
>Mature_239_residues
MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDV
IVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYCQ

Specific function: Member of the two-component regulatory system dcuR/dcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-di

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1790566, Length=239, Percent_Identity=100, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1786838, Length=220, Percent_Identity=31.3636363636364, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCUR_ECO57 (P0AD02)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91267
- PIR:   G86107
- RefSeq:   NP_290757.1
- RefSeq:   NP_313133.1
- ProteinModelPortal:   P0AD02
- SMR:   P0AD02
- EnsemblBacteria:   EBESCT00000024166
- EnsemblBacteria:   EBESCT00000059404
- GeneID:   914216
- GeneID:   960000
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5726
- KEGG:   ecs:ECs5106
- GeneTree:   EBGT00050000008649
- HOGENOM:   HBG753323
- OMA:   IKPFEFE
- ProtClustDB:   PRK10430
- BioCyc:   ECOL83334:ECS5106-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR012287
- InterPro:   IPR001789
- Gene3D:   G3DSA:1.10.10.60
- SMART:   SM00448

Pfam domain/function: PF00072 Response_reg; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 27489; Mature: 27489

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQ
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEE
KENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGW
CCCCCEEHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLTPQTLRTLCQWIDAHQDYEFS
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
TDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYCQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHCC
>Mature Secondary Structure
MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQ
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEE
KENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGW
CCCCCEEHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLTPQTLRTLCQWIDAHQDYEFS
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
TDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYCQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796