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The map label for this gene is prtB [H]

Identifier: 209398588

GI number: 209398588

Start: 2434904

End: 2436964

Strand: Reverse

Name: prtB [H]

Synonym: ECH74115_2579

Alternate gene names: 209398588

Gene position: 2436964-2434904 (Counterclockwise)

Preceding gene: 209397073

Following gene: 209400706

Centisome position: 43.74

GC content: 49.88

Gene sequence:

>2061_bases
ATGCTACCAAAAGCCGCCCGCATTCCCCACGCCATGACGCTTCATGGCGATACGCGCATCGATAATTACTACTGGCTGCG
GGACGATACGCGTTCTCAGCCGGAAGTCCTGGACTACCTGCAACAAGAAAATAGTTACGGTCATCGGGTGATGGCCTCAC
AACAAGCCTTGCAGGATCGCATCTTAAAGGAAATCATCGACCGCATTCCGCAACGAGAAGTTTCTGCGCCCTACATCAAA
AATGGCTACCGCTACCGGCATATTTATGAACCAGGCTGTGAATATGCTATCTACCAGCGTCAATCGGCGTTCAGTGAAGA
GTGGGACGAGTGGGAAATATTGCTCGATGCCAACAAGCGCGCGGCTCATAGTGAGTTTTATTCGATGGGCGGAATGGCGA
TTACGCCCGATAACACCATTATGGCGCTGGCAGAAGATTTTCTTTCCCGACGCCAGTACGGCATTCGTTTTCGTAATCTG
GAAACAGGTAACTGGTACCCGGAACTGCTGGATAACGTTGAACCCAGCTTTGTCTGGGCAAATGACTCCTGGACTTTCTA
CTATGTTCGCAAGCATCCGGTGACGCTGCTGCCTTATCAGGTCTGGCGTCACGCCATCGGTACGCCAGCATCGCAAGATA
AACTGATTTACGAAGAAAAAGACGATACCTATTACGTCAGCCTGCATAAAACGACCTCAAAGCACTATGTAGTCATTCAT
TTGGCCAGCGCCACCACCAGTGAAGTTCGCCTGCTGGACGCGGAAATGGCAGATGCCGAGCCGTTTGTTTTTCTGCCGCG
CCGCAAAGATCACGAATACAGCCTTGATCACTACCAGCATCGTTTTTATCTGCGTTCCAACCGCCACGGCAAAAACTTTG
GCTTATACCGTACCCGTATGCGTGATGAGCAACAGTGGGAAGAGTTAATTCCGCCACGCGAAAACATCATGCTGGAAGGG
TTTACGCTGTTTACCGACTGGCTGGTGGTTGAAGAGCGTCAGCGCGGGTTAACCAGTTTGCGCCAAATTAACCGCAAGAC
CCGGGAAGTCATTGGTATTGCCTTTGATGATCCAGCCTATGTGACCTGGATTGCCTACAATCCAGAACCTGAAACCGCGC
GATTGCGTTATGGTTATTCTTCCATGACCACACCAGACACTTTGTTTGAACTGGATATGGATACCGGTGAGCGTCGTGTA
TTAAAACAAACAGAAGTTCCTGGTTTTGATGCGGCGAATTACCGCAGTGAACACCTGTGGATAGTCGCCCGTGATGGTGT
CGAAGTTCCGGTTTCGTTGGTCTACCATCGCAATCATTTTCGCAAAGGACACAACCCGCTGCTGGTGTATGGCTACGGTT
CTTACGGCGCAAGTATTGATGCCGATTTCAGTTTTAGCCGCTTGAGTTTGTTAGATCGTGGCTTTGTCTACGCCATTGTC
CATGTTCGCGGCGGCGGTGAGCTGGGGCAACAATGGTACGAAGACGGTAAATTTCTGAAGAAGAAAAATACGTTTAATGA
TTATCTTGATGCCTGCGATGCATTGTTAAAACTGGGCTATGGCTCTCCTTCGCTTTGTTATGCGATGGGCGGGAGTGCGG
GGGGCATGTTGATGGGCGTTGCAATTAATCAACGCCCGGAATTATTCCACGGCGTTATCGCCCAGGTACCGTTTGTTGAT
GTTGTAACAACGATGCTTGATGAATCAATTCCTCTTACCACTGGTGAGTTTGAAGAGTGGGGGAATCCGCAGGATCTGCA
ATATTACGAGTATATGAAAAGCTACAGCCCGTATGACAACGTCACCGCACAGGCTTATCCACATTTACTGGTAACGACCG
GTTTACACGATTCTCAGGTGCAATATTGGGAACCGGCAAAATGGGTCGCTAAGTTGCGCGAGCTGAAAACCGATAACCAT
CTTTTATTGCTCTGTACCGACATGGACTCAGGCCATGGCGGTAAATCTGGTCGCTTTAAATCGTACGAAGGCGTAGCGAT
GGAATATGCTTTTCTGGTCGCGCTGGCGCAGGGAACATTACCCGCTAGGCCTGCGGATTAG

Upstream 100 bases:

>100_bases
ATTACCTGTCATCATCTAAGCAATGACTGCCCTGTTTCGCTTGCATCCCCGGTGAGTTTTGCCACCCTTATAAGATGTTT
CAACCAGAAAGAACAATAAC

Downstream 100 bases:

>100_bases
GTATTTTCCAGATAATGTTTCAGTGTTAAACGCAGCTCCGGGCTCATGCTGTCCAGGTTATTAAACAACCAGCGCAGATA
GCCCGGATCGCGTTCGGCAA

Product: protease 2

Products: NA

Alternate protein names: Oligopeptidase B; Protease II [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIK
NGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEG
FTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRV
LKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVD
VVTTMLDESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNH
LLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPARPAD

Sequences:

>Translated_686_residues
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIK
NGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEG
FTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRV
LKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVD
VVTTMLDESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNH
LLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPARPAD
>Mature_686_residues
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIK
NGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEG
FTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRV
LKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVD
VVTTMLDESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNH
LLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPARPAD

Specific function: Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [H]

COG id: COG1770

COG function: function code E; Protease II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9A family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=696, Percent_Identity=25, Blast_Score=200, Evalue=4e-51,
Organism=Homo sapiens, GI284172420, Length=474, Percent_Identity=29.957805907173, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI284172413, Length=474, Percent_Identity=29.957805907173, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI70778815, Length=474, Percent_Identity=29.957805907173, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI284172438, Length=474, Percent_Identity=29.957805907173, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI284172431, Length=474, Percent_Identity=29.957805907173, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI108860686, Length=214, Percent_Identity=40.6542056074766, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI108860692, Length=213, Percent_Identity=40.8450704225352, Blast_Score=149, Evalue=1e-35,
Organism=Escherichia coli, GI1788150, Length=686, Percent_Identity=98.9795918367347, Blast_Score=1413, Evalue=0.0,
Organism=Drosophila melanogaster, GI24583414, Length=701, Percent_Identity=23.1098430813124, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI221510989, Length=699, Percent_Identity=25.4649499284692, Blast_Score=155, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002471
- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: =3.4.21.83 [H]

Molecular weight: Translated: 79499; Mature: 79499

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00708 PRO_ENDOPEP_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDR
CCCCCCCCCEEEEECCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
ILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKR
HHHHHHHHCCCCCCCCCHHHCCEEEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCC
AAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYPELLDNVEPSFVWA
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEEEE
NDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
CCCEEEEEEECCCCEEECHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEEE
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTRM
EECCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCHHHEEEEEECCCCCCCCCEEHHHC
RDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAY
CCHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
VTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLW
EEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCEEE
IVARDGVEVPVSLVYHRNHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
EEEECCCCCCEEEEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEEE
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGV
EEECCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEE
AINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDN
EECCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCC
VTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFK
CCHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
SYEGVAMEYAFLVALAQGTLPARPAD
CCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDR
CCCCCCCCCEEEEECCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
ILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKR
HHHHHHHHCCCCCCCCCHHHCCEEEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCC
AAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYPELLDNVEPSFVWA
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEEEE
NDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
CCCEEEEEEECCCCEEECHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEEE
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTRM
EECCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCHHHEEEEEECCCCCCCCCEEHHHC
RDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAY
CCHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
VTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLW
EEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCEEE
IVARDGVEVPVSLVYHRNHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
EEEECCCCCCEEEEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEEE
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGV
EEECCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEE
AINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDN
EECCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCC
VTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFK
CCHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
SYEGVAMEYAFLVALAQGTLPARPAD
CCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1769955; 9097040; 9278503 [H]