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The map label for this gene is amyA [H]

Identifier: 209398533

GI number: 209398533

Start: 2536288

End: 2537775

Strand: Direct

Name: amyA [H]

Synonym: ECH74115_2702

Alternate gene names: 209398533

Gene position: 2536288-2537775 (Clockwise)

Preceding gene: 209399299

Following gene: 209399347

Centisome position: 45.52

GC content: 49.46

Gene sequence:

>1488_bases
ATGCGTAATCCCACGCTGTTACAATGTTTTCACTGGTATTACCCGGAAGGCGGTAAGCTCTGGCCTGAATTGGCCGAGCG
CGCCGACGGTTTTAATGATATTGGTATCAATATGGTCTGGTTGCCGCCCGCCTATAAAGGCGCATCGGGCGGGTATTCGG
TCGGCTACGACTCCTATGATTTATTTGATTTAGGTGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAA
GTACAACTGCTGGCCGCCATTGACGCCCTGAAACGTAATGACATTGCGGTGCTGTTGGACGTGGTAGTCAACCACAAAAT
GGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGCGTTAATGCTGATGACCGTACGCAAATTGATGAAGAAATCATTG
AATGCGAAGGCTGGACGCGTTACACCTTCCCCGCTCGTGCCGGGCAATACTCACAGTTTATCTGGGATTTCAAATGTTTT
AGCGGTATCGACCATATCGAAAACCCCAATGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAA
CGATCAGGTTGATGATGAACTCGGTAATTTCGATTATCTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGG
AAGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATT
CCAGCCTGGTTTTATAAAGAGTGGATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCGGAGTACTG
GTCGCATGAAGTTGATAAGCTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGA
TGAAATTCCATGAAGCATCGCGCATGGGACGCAACTACGACATGACGCAGATTTTCACTGGGACGCTGGTGGAAGCCGAT
CCTTTTCACGCCGTAACCCTCGTTGCCAACCACGACACCCAACCGTTGCAAGCCCTCGAAGCACCGGTCGAACCGTGGTT
TAAACCGCTGGCGTATGCTCTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTGTTCTATCCGGACCTCTACGGTGCGC
ATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTC
GCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAC
CGACGAATATCCCGGCTGCGTGGTGGTCATGTCGAACGGAGATGATGGCGAAAAAACCATTCATTTAGGAGAGAATTACG
GCAATAAAACCTGGCGTGATTTTCTTGGGAACCGGCAAGAGAGTGTAGTAACCGACGAAAACGGCGAAGCGACCTTCTTT
TGCAACGGCGGCAGCGTTAGCGTGTGGGTTATCGAAGAGGTGCTTTAA

Upstream 100 bases:

>100_bases
CTCCGCAGGATAACCTCTTTTGAATCTGAATGAGTCGATGGCTCGCGAATAATCCGATTACGGCTACGCTTCTAATGTTC
CCCTTGAATGGAGTCGAAGA

Downstream 100 bases:

>100_bases
ATTCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAATGGCGTCGGTAACGGCGCTTTATCCAGCGCAGCGGCAC
ACTCTGCCGTCGGGCGATCC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDK
VQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
PAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELIL
ARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
CNGGSVSVWVIEEVL

Sequences:

>Translated_495_residues
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDK
VQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
PAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELIL
ARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
CNGGSVSVWVIEEVL
>Mature_495_residues
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDK
VQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
PAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELIL
ARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
CNGGSVSVWVIEEVL

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=495, Percent_Identity=98.7878787878788, Blast_Score=1021, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56613; Mature: 56613

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYD
CCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHCEEEEEC
NADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIENPNEDGIFKIVND
CCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEC
YTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
CCCCCCCCCHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHH
RMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
HCCCCCCHHHEEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHEEECC
GVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELILARQRFAHGVQTLFFDHPNCI
CCCCEECCCCCCCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
AFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCEEEE
CNGGSVSVWVIEEVL
ECCCCEEEEEEEECC
>Mature Secondary Structure
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYD
CCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHCEEEEEC
NADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIENPNEDGIFKIVND
CCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEC
YTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
CCCCCCCCCHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHH
RMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
HCCCCCCHHHEEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHEEECC
GVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELILARQRFAHGVQTLFFDHPNCI
CCCCEECCCCCCCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
AFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCEEEE
CNGGSVSVWVIEEVL
ECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 9097040; 9278503; 1527488; 8371104 [H]