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The map label for this gene is ybfL [H]

Identifier: 209398400

GI number: 209398400

Start: 667877

End: 669013

Strand: Reverse

Name: ybfL [H]

Synonym: ECH74115_0643

Alternate gene names: 209398400

Gene position: 669013-667877 (Counterclockwise)

Preceding gene: 209400670

Following gene: 209400631

Centisome position: 12.01

GC content: 41.42

Gene sequence:

>1137_bases
ATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCTGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATC
GGATATTCTACTGTTGACTATTTGTGCCGTCATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTAGGGGAAACAC
ATCTCAATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGT
ATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGC
AATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGAGAGCGATTCATGTCATTAGTGCGTTCTCAA
CAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAAC
ATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACA
GGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAG
AATTAAATAATCCAGAGCATGACAGTTACGCAATGAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTT
TGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCG
GTCCATAATAGCAGAACAAAAGAAAGAGTCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAGA
AGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAGTGAATGAAGAC
GACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGA
TAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCTTTGCGG
GGAGCGGGCTTTCGTAA

Upstream 100 bases:

>100_bases
GCACTAAATGTTCAATAATTGAGATATTCCTTATTATCTAAAACTGTTTTTCACAGCTTACATATGATCAAATACTCCTT
ACATAATTAAGGAGAACAAA

Downstream 100 bases:

>100_bases
TCTTGCCCTGCCTGCTCACCTCCAGGCACTTCCACTACATCATGACCTGATCGGTGCCACCACTCCTCTTCACTATGACG
TTAACCTCCCCTCCTTGACA

Product: ISEc4, transposase

Products: NA

Alternate protein names: H repeat-associated protein in rhsC-phrB intergenic region; ORF-H2 [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDLGETHLNFLKQYGDFENGIPVHDTIARVVSC
ISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRRAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIV
CDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNED
DCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS

Sequences:

>Translated_378_residues
MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDLGETHLNFLKQYGDFENGIPVHDTIARVVSC
ISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRRAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIV
CDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNED
DCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS
>Mature_378_residues
MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDLGETHLNFLKQYGDFENGIPVHDTIARVVSC
ISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRRAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIV
CDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNED
DCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS

Specific function: Unknown

COG id: COG5433

COG function: function code L; Transposase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1789896, Length=378, Percent_Identity=97.6190476190476, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI1787733, Length=378, Percent_Identity=97.0899470899471, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1786924, Length=253, Percent_Identity=93.6758893280632, Blast_Score=490, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 43203; Mature: 43203

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDLGETHLNFLKQ
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKS
HCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
RRRRAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
AEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIV
HHHHHHCCCCEEEEEECCCCCCHHHHHHCCCHHHCCCCCCCCHHHHHHHCCCEEEEEEEE
CDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKESEMTVRYYISSADLTAEKFATAIR
ECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHH
NHWHVENKLHWRLDVVVNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRK
HCCCCCCEEEEEEEEEEECCCCCEECCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHH
AAMDRNYLASVFAGSGLS
HHHCHHHHHHHHCCCCCC
>Mature Secondary Structure
MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDLGETHLNFLKQ
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKS
HCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
RRRRAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
AEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIV
HHHHHHCCCCEEEEEECCCCCCHHHHHHCCCHHHCCCCCCCCHHHHHHHCCCEEEEEEEE
CDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKESEMTVRYYISSADLTAEKFATAIR
ECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHH
NHWHVENKLHWRLDVVVNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRK
HCCCCCCEEEEEEEEEEECCCCCEECCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHH
AAMDRNYLASVFAGSGLS
HHHCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8387990; 8905232; 9278503 [H]