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The map label for this gene is insI1 [H]

Identifier: 209398359

GI number: 209398359

Start: 3594672

End: 3595427

Strand: Direct

Name: insI1 [H]

Synonym: ECH74115_3886

Alternate gene names: 209398359

Gene position: 3594672-3595427 (Clockwise)

Preceding gene: 209396319

Following gene: 209399308

Centisome position: 64.51

GC content: 46.69

Gene sequence:

>756_bases
ATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGC
GAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGC
GGGCTGTAGCTCACCTGACACTGTCTGAGCGCGAGGAGATACGAGCTGGTTTGTCAGCCAAAATGAGCATTCGTGCGATA
GCTACTGCGCTGAATCGCAGTCCTTCGACGATCTCACGTGAAGTTCAGCGTAATCGGGGCAGACGCTATTACAAAGCTGT
TGATGCTAATAACCGAGCCAACAGAATGGCGAAAAGGCCAAAACCGTGCTTACTGGATCAAAATTTACCATTGCGAAAGC
TTGTTCTGGAAAAGCTGGAGATGAAATGGTCTCCAGAGCAAATATCAGGATGGTTAAGGCGAACAAAACCACGTCAAAAA
ACGCTGCGAATATCACCTGAGACAATTTATAAAACGCTGTACTTTCGTAGCCGTGAAGCGCTACACCACCTGAATATACA
GCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGA
ACGGAACACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTAGGGCATTGGGAGGGCGATTTAGTCTCA
GGTACAAAAAACTCTCATATAGATTCGTTTTTCTGA

Upstream 100 bases:

>100_bases
GAAATAGAAATAGAAATAGAAATATTAAAACAGACCACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATG
GGGTTGCGGAGGTTTTTTGA

Downstream 100 bases:

>100_bases
CCTTGGCAGGTTGTTCTTCAGTAGGCAACCAGTCATTGAAAAATGAGACGCAGGAAAGTGTGAAAACCAAAATTGTTAAA
GGCAAAACTACAAAACAGGA

Product: transposase InsI for insertion sequence element IS30B/C/D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIDSFF

Sequences:

>Translated_251_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIDSFF
>Mature_251_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIDSFF

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=251, Percent_Identity=98.406374501992, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1787671, Length=251, Percent_Identity=98.406374501992, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1786450, Length=251, Percent_Identity=98.406374501992, Blast_Score=505, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29114; Mature: 29114

Theoretical pI: Translated: 11.92; Mature: 11.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEHCCCCCHHHHHHCCC
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHHHH
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHCCCCCCCCCCC
GTKNSHIDSFF
CCCCCCCCCCC
>Mature Secondary Structure
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEHCCCCCHHHHHHCCC
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHHHH
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHCCCCCCCCCCC
GTKNSHIDSFF
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]