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The map label for this gene is ysgA

Identifier: 209398246

GI number: 209398246

Start: 4914778

End: 4915593

Strand: Reverse

Name: ysgA

Synonym: ECH74115_5271

Alternate gene names: 209398246

Gene position: 4915593-4914778 (Counterclockwise)

Preceding gene: 209396563

Following gene: 209399435

Centisome position: 88.22

GC content: 54.41

Gene sequence:

>816_bases
ATGGCAACAACACAACAATCTGGATTTGCACCTGCTGCATCGCCTCTCGCTTCGACCATCGTTCAGACCCCGGACGACGC
GATTGTGGCGGGCTTCACCTCTATCCCTTCACAAGGGGATAACATGCCTGCTTACCATGCCAGACCAAAGCAAAGCGATG
GCCCACTGCCAGTGGTCATTGTAGTGCAGGAAATTTTTGGCGTGCATGAACATATCCGCGACATTTGTCGCCGTCTGGCG
CTGGAGGGGTATCTGGCTATCGCACCTGAACTTTACTTCCGCGAAGGCGATCCGAATGATTTTGCCGATATCCCCACGTT
GCTTAGCGGTCTGGTAGCAAAAGTGCCTGACTCGCAGGTGCTGGCCGATCTCGATCATGTCGCCAGTTGGGCTTCCCGCA
ACGGCGGCGATGTTCATCGTTTAATGATCACCGGATTCTGCTGGGGTGGACGTATCACTTGGCTGTATGCCGCGCATAAT
CCACAGCTAAAAGCCGCAGTGGCGTGGTACGGCAAACTGACGGGCGATAAGTCGCTGAATTCACCGAAACAACCTGTTGA
TATCGCAACCGATCTTAACGCGCCGGTTCTCGGCTTATATGGCGGTCTGGATAACAGTATTCCGCAGGAGAGCGTTGAAA
CCATGCGCCAGGCGCTGCGGGCTGCTAATGCGAAAGCAGAGATTATCGTGTACCCGGACGCCGGGCATGCATTCAACGCT
GATTATCGCCCGAGCTATCATGCCGCATCTGCAGAAGATGGCTGGCAGCGTATGCTGGAATGGTTTAAGCAGTATGGTGG
GAAGAAGTCGCTGTAA

Upstream 100 bases:

>100_bases
AGGACTGCATAAAAGATGAGCCGATTCAGGCAATGTGATTTGTTGCATGCTGACTATAGTTAACATTGCGCAACATTTTT
ATAAGGGTACGGAGAATACC

Downstream 100 bases:

>100_bases
TGAGAAAAGACCGGGTGGTATTACCACCCGGTTTGGATTTTACCCCCGACGCAAGTTCTGCGCCGCCTGCACCATGTTCG
CCAGTGCCGCGCGGGTTTCT

Product: dienelactone hydrolase family protein

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLA
LEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFNA
DYRPSYHAASAEDGWQRMLEWFKQYGGKKSL

Sequences:

>Translated_271_residues
MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLA
LEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFNA
DYRPSYHAASAEDGWQRMLEWFKQYGGKKSL
>Mature_270_residues
ATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLAL
EGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNP
QLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFNAD
YRPSYHAASAEDGWQRMLEWFKQYGGKKSL

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family

Homologues:

Organism=Escherichia coli, GI48994982, Length=271, Percent_Identity=99.2619926199262, Blast_Score=556, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DLHH_ECO57 (Q8X8L4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F86070
- PIR:   H91223
- RefSeq:   NP_290462.1
- RefSeq:   NP_312787.1
- ProteinModelPortal:   Q8X8L4
- SMR:   Q8X8L4
- EnsemblBacteria:   EBESCT00000025675
- EnsemblBacteria:   EBESCT00000060081
- GeneID:   915144
- GeneID:   960328
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5352
- KEGG:   ecs:ECs4760
- GeneTree:   EBGT00050000010527
- HOGENOM:   HBG673102
- OMA:   VHEHIAD
- ProtClustDB:   CLSK880795
- BioCyc:   ECOL83334:ECS4760-MONOMER
- InterPro:   IPR002925

Pfam domain/function: PF01738 DLH

EC number: =3.1.1.45

Molecular weight: Translated: 29396; Mature: 29265

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: ACT_SITE 147-147 ACT_SITE 204-204 ACT_SITE 236-236

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVVI
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEECHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
VVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQV
HHHHHHHHHHHHHHHHHHHHHCCHHEECCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHH
LADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGKLTGDKSLN
HHHHHHHHHHHHCCCCCEEEEEEEEEEECCEEEEEEECCCCCCEEHHHHHHHCCCCCCCC
SPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFNA
CCCCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
DYRPSYHAASAEDGWQRMLEWFKQYGGKKSL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVVI
CCCCCCCCCCCHHHHHHHHHCCCCCCEEECHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
VVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQV
HHHHHHHHHHHHHHHHHHHHHCCHHEECCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHH
LADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGKLTGDKSLN
HHHHHHHHHHHHCCCCCEEEEEEEEEEECCEEEEEEECCCCCCEEHHHHHHHCCCCCCCC
SPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFNA
CCCCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
DYRPSYHAASAEDGWQRMLEWFKQYGGKKSL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796