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The map label for this gene is nsr [H]

Identifier: 209398067

GI number: 209398067

Start: 3031606

End: 3032304

Strand: Direct

Name: nsr [H]

Synonym: ECH74115_3299

Alternate gene names: 209398067

Gene position: 3031606-3032304 (Clockwise)

Preceding gene: 209400797

Following gene: 209397420

Centisome position: 54.41

GC content: 43.2

Gene sequence:

>699_bases
GTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGTTTAAGGATTGCTTTTTAACGGATGT
TTCAGCCGATACGCGGCTGTTTCATTTTTTAGCGCGTGACTACATTGTGCAGGAAGGGCAACAGCCGTCCTGGCTGTTTT
ACCTGACGCGAGGCCGCGCCAGGCTTTACGCCACGCTAGCTAATGGTCGCGTGTCGCTGATCGATTTCTTTGCCGCCCCC
TGTTTTATTGGTGAGATTGAGTTAATCGATAAAGACCATGAACCGCGTGCGGTACAGGCTATTGAAGAGTGTTGGTGCCT
TGCGCTCCCTATGAAACATTACCGTCCGCTGTTATTAAACGACACCCTATTTTTACGAAAACTTTGCGTCACCTTAAGTC
ATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTA
CTCTCACAAGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTT
ATATGTTCTCGCGCAATTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGATATCTAATTAAAAACAGAAAGCAGT
TGTCAGGGCTGGCACTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAA

Upstream 100 bases:

>100_bases
ATTTTTTCTACAGCAAAGAAATTTCGATCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAA
TCTCATAGCGGATAAACATC

Downstream 100 bases:

>100_bases
AAATTATTTGCAATAGCGCGATTGCCGGATGCAACGCTTGATGCGTTTTATCCGGTCTACAAATTGAGCATTACGCCAGA
CCAATAAAGAACCCGGCAAT

Product: DNA-binding transcriptional activator YeiL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAP
CFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ

Sequences:

>Translated_232_residues
MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAP
CFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
>Mature_232_residues
MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAP
CFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ

Specific function: Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as genera

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788487, Length=219, Percent_Identity=100, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 26888; Mature: 26888

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCE
RLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLN
EEEEEECCCCCHHHHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCC
DTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLG
CHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHC
VSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
CHHHHHHHHHHHHHHCCEEEECCCCCEEECCHHHCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCE
RLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLN
EEEEEECCCCCHHHHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCC
DTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLG
CHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHC
VSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
CHHHHHHHHHHHHHHCCEEEECCCCCEEECCHHHCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]