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The map label for this gene is glpK [H]

Identifier: 209398014

GI number: 209398014

Start: 4459820

End: 4461328

Strand: Direct

Name: glpK [H]

Synonym: ECH74115_4826

Alternate gene names: 209398014

Gene position: 4459820-4461328 (Clockwise)

Preceding gene: 209397224

Following gene: 209396982

Centisome position: 80.04

GC content: 54.34

Gene sequence:

>1509_bases
ATGCCTGATAACAGCGCAGCTATCGTTATCGATATTGGCACCACCAATTGCAAAGTCACCTGCTTTTCCTGCCTGGACGC
AACGACGTTGGGCGCGCATAAATTCGTGACGGCAAAGCAGATCTCCCCACAGGGCGATGTCGATTTCGATATCGACGCCC
TCTGGCAGGAGGTCCGCCAGGCGATAGCGCAACTGAACGCCGCTTCGCCGCTGCCAGTCAGACGGATCAGCATTGCCAGT
TTTGGCGAATCAGGCGTGTTCCTTGACGAGCATGGCGAGATCCTGACGCCAATGCTGGCATGGTATGACCGTCGCGGTGA
AGAGTATCTGGCAACGCTTAGCGAGGCAGACAGTGCGGCACTTTATGACATCTGCGGTCTACCACTACACAGCAATTACT
CTGCCTTCAAAATGCGCTGGTTGCTGGAACATTACCCGCTGCGTAATCGCCGCGGCCTGCGCTGGCTACATGCGCCGGAA
GTGCTGCTCTGGCGGCTGACTGGCGAACAGCACACGGATATCACCTTAGCCAGCCGCACGCTGTGTCTGGACGTGCGCAA
AGGCGAATGGTCAGCGAAAGCGGCGGCGTTGTTACACGTTCCCTGTACGGCATTTGCGCCATTGGTGCAGCCAGGCGAGC
ACGCCGGATGGGTCAGCGAGTCACTTTGCAAGACGCTTGGGTTCTCGCAACCGGTCAGCGTGACGCTGGCCGGACATGAC
CATATGGTGGGTGCGCGAGCGTTGCAGATGATGCCAGGCGATATCCTTAACTCGACGGGGACCACGGAAGGCATTCTGCA
ACTGGATACACAACCGACGCTGGATGAACAGGCCAAACGTGACAAGCTGGCAAACGGCTGTTACTCACTTGCCAACCAGT
TCACCCTGTTTGCGTCGCTGCCCGTGGGCGGTTTCGCGCTGGAGTGGCTGCGCAACACGTTCCGGCTAACCGATGAGGAG
ATCGCCGCATCACTTACTCGCGGACATGCTGATTATCTGGCGGGGAATTGGTTGCTCGATGACATTCCCGTCTTTATTCC
ACATCTTCGCGGTTCGGGTTCGCCCTATAAAAATCGCCATACCCGTGGATTATTTTATGGGCTTGGCGATACGTTAAGTA
TCGACATGTTAATTGCCAGCGTATCACTGGGATTAACCATGGAATTTGCCAACTGCTTCGCCTGTTTTAACGTGCCTGGC
ACCAGCGCGTTAAAAGTGATCGGTCCGGCAACCCATAATCCTCTTTGGCTGCAATTAAAGGCGGATATTTTACAGCGTCC
GGTTGAAGCAATTGCATTTAACGAGGCGGTTTCTGTCGGAGCATTATTAACCGCCGCACCGGATATTCCACCGCCGCCAG
TCGCTATAGCCCAACGTTTGTTACCGAATCGGGCGAGATACCATCAATTACAGCGTTATCAGCACAAATGGAAAAGCTGG
TATCAGTTGAAATTACAACAAGAAGGCGTGATGCCATTACATCATCGAGAGGAACACTATGTTGAATAA

Upstream 100 bases:

>100_bases
CTGCTGGAAATCTTCAACGGCAATCTCATCGCCATTGGTCTGGTGCCGGTTATCGCCCTGGTACTGTATGGCATTTTCCG
AATGACGCGGAGCACGGTTT

Downstream 100 bases:

>100_bases
AACCGTGGAGGTCAGAAACAGTACTGGCCTGCATGCACGTCCGGCGGCCTGTCTGGCGAAAGCCGCCAAAAAATATAGCT
GTAAAGTGACGCTGCACTAT

Product: carbohydrate kinase, FGGY family

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIAS
FGESGVFLDEHGEILTPMLAWYDRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEE
IAASLTRGHADYLAGNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANCFACFNVPG
TSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIPPPPVAIAQRLLPNRARYHQLQRYQHKWKSW
YQLKLQQEGVMPLHHREEHYVE

Sequences:

>Translated_502_residues
MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIAS
FGESGVFLDEHGEILTPMLAWYDRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEE
IAASLTRGHADYLAGNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANCFACFNVPG
TSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIPPPPVAIAQRLLPNRARYHQLQRYQHKWKSW
YQLKLQQEGVMPLHHREEHYVE
>Mature_501_residues
PDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASF
GESGVFLDEHGEILTPMLAWYDRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPEV
LLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHDH
MVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEI
AASLTRGHADYLAGNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANCFACFNVPGT
SALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIPPPPVAIAQRLLPNRARYHQLQRYQHKWKSWY
QLKLQQEGVMPLHHREEHYVE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG1070

COG function: function code G; Sugar (pentulose and hexulose) kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=453, Percent_Identity=24.0618101545254, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI41393575, Length=447, Percent_Identity=24.1610738255034, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4504007, Length=451, Percent_Identity=23.9467849223947, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI189409120, Length=451, Percent_Identity=23.9467849223947, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789168, Length=460, Percent_Identity=26.304347826087, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789987, Length=490, Percent_Identity=22.6530612244898, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=447, Percent_Identity=23.7136465324385, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17864214, Length=538, Percent_Identity=23.7918215613383, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24654573, Length=464, Percent_Identity=24.5689655172414, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922058, Length=339, Percent_Identity=23.3038348082596, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55576; Mature: 55445

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQ
CCCCCCEEEEEECCCCCEEEEEEHHCCHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHH
AIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWYDRRGEEYLATLSEADSAA
HHHHCCCCCCCCEEEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHH
LYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPEVLLWRLTGEQHTDITLASRT
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEECEE
LCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
EEEEECCCCCCCCEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCC
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASL
CHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEC
PVGGFALEWLRNTFRLTDEEIAASLTRGHADYLAGNWLLDDIPVFIPHLRGSGSPYKNRH
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCHHCHHHHHHCCCCCCCCCCCCC
TRGLFYGLGDTLSIDMLIASVSLGLTMEFANCFACFNVPGTSALKVIGPATHNPLWLQLK
CCEEEEECCCCHHHHHHHHHHHHCEEEEHHCEEEEECCCCCCEEEEECCCCCCCEEEEEE
ADILQRPVEAIAFNEAVSVGALLTAAPDIPPPPVAIAQRLLPNRARYHQLQRYQHKWKSW
HHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
YQLKLQQEGVMPLHHREEHYVE
EEEEEECCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQ
CCCCCEEEEEECCCCCEEEEEEHHCCHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHH
AIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWYDRRGEEYLATLSEADSAA
HHHHCCCCCCCCEEEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHH
LYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPEVLLWRLTGEQHTDITLASRT
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEECEE
LCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
EEEEECCCCCCCCEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCC
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASL
CHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEC
PVGGFALEWLRNTFRLTDEEIAASLTRGHADYLAGNWLLDDIPVFIPHLRGSGSPYKNRH
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCHHCHHHHHHCCCCCCCCCCCCC
TRGLFYGLGDTLSIDMLIASVSLGLTMEFANCFACFNVPGTSALKVIGPATHNPLWLQLK
CCEEEEECCCCHHHHHHHHHHHHCEEEEHHCEEEEECCCCCCEEEEECCCCCCCEEEEEE
ADILQRPVEAIAFNEAVSVGALLTAAPDIPPPPVAIAQRLLPNRARYHQLQRYQHKWKSW
HHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
YQLKLQQEGVMPLHHREEHYVE
EEEEEECCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]