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The map label for this gene is rsmI

Identifier: 209397996

GI number: 209397996

Start: 4136740

End: 4137600

Strand: Reverse

Name: rsmI

Synonym: ECH74115_4465

Alternate gene names: 209397996

Gene position: 4137600-4136740 (Counterclockwise)

Preceding gene: 209396489

Following gene: 209398713

Centisome position: 74.26

GC content: 54.47

Gene sequence:

>861_bases
ATGAAACAACACCAATCGGCGGATAATTCTCAGGGCCAGCTTTACATTGTACCGACGCCAATCGGCAATCTGGCGGATAT
CACCCAGCGTGCGTTAGAGGTATTACAGGCCGTTGATCTGATTGCCGCCGAGGATACTCGTCACACCGGTTTATTGCTGC
AACATTTTGGGATTAATGCCCGGTTGTTTGCGCTGCACGACCATAACGAACAACAAAAAGCCGAAACGCTGCTGGCGAAG
CTGCAAGAGGGGCAAAACATTGCGCTGGTTTCCGATGCCGGAACGCCGCTAATTAACGATCCTGGCTACCATCTGGTGCG
TACCTGCCGTGAAGCGGGGATTCGTGTGGTGCCGCTACCCGGACCGTGTGCTGCTATCACTGCGTTAAGCGCCGCGGGTT
TACCCTCTGACCGTTTCTGTTACGAAGGCTTTTTACCTGCCAAATCAAAAGGCCGCCGTGATGCGCTAAAAGCCATTGAA
GCGGAGCCGCGCACGCTGATTTTTTATGAATCTACCCACCGTCTGTTAGATAGCCTGGAAGATATCGTTGCGGTATTAGG
CGAATCCCGCTACGTGGTTCTGGCGCGTGAGCTGACCAAAACCTGGGAAACCATTCACGGCGCGCCCGTTGGCGAGCTGC
TGGCGTGGGTAAAGGAAGATGAAAACCGTCGCAAAGGCGAAATGGTGCTGATTGTCGAAGGTCATAAAGCACAGGAAGAA
GACTTACCCGCCGATGCCCTGCGCACGCTGGCGCTGCTACAGGCAGAACTGCCGCTGAAAAAAGCGGCGGCGCTGGCCGC
AGAAATTCACGGCGTGAAGAAAAATGCGCTGTATAAGTATGCGCTGGAGCAGCAGGGGTAA

Upstream 100 bases:

>100_bases
CGCGGCTTTCAAACGAGAAAATGTTGAGGGTACCATAATGTATCCAGTGATATTTTTTTTACGCAATGCTCAATATTAAA
TCGGCAATACGGACGACACA

Downstream 100 bases:

>100_bases
GAGTGCCGAAGCGTTGCGTTAATCAAAACGCAACGTCTTCTTTTACCGCACGATGACGGTAATGGTGGCTGTGCCCTGAA
ATTTTCCTGTTTCCAGCTCA

Product: tetrapyrrole methylase family protein

Products: NA

Alternate protein names: 16S rRNA 2'-O-ribose C1402 methyltransferase; rRNA (cytidine-2'-O-)-methyltransferase RsmI

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAK
LQEGQNIALVSDAGTPLINDPGYHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVKEDENRRKGEMVLIVEGHKAQEE
DLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKYALEQQG

Sequences:

>Translated_286_residues
MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAK
LQEGQNIALVSDAGTPLINDPGYHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVKEDENRRKGEMVLIVEGHKAQEE
DLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKYALEQQG
>Mature_286_residues
MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAK
LQEGQNIALVSDAGTPLINDPGYHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVKEDENRRKGEMVLIVEGHKAQEE
DLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKYALEQQG

Specific function: Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA

COG id: COG0313

COG function: function code R; Predicted methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmI family

Homologues:

Organism=Escherichia coli, GI1789535, Length=286, Percent_Identity=100, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMI_ECO57 (P67088)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91132
- PIR:   F85977
- RefSeq:   NP_289722.1
- RefSeq:   NP_312054.1
- ProteinModelPortal:   P67088
- SMR:   P67088
- PRIDE:   P67088
- EnsemblBacteria:   EBESCT00000026925
- EnsemblBacteria:   EBESCT00000059148
- GeneID:   916135
- GeneID:   958599
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4505
- KEGG:   ecs:ECs4027
- GeneTree:   EBGT00050000009537
- HOGENOM:   HBG529058
- OMA:   ASDRFCF
- ProtClustDB:   PRK14994
- BioCyc:   ECOL83334:ECS4027-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01877
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR008189
- InterPro:   IPR018063
- Gene3D:   G3DSA:3.40.1010.10
- TIGRFAMs:   TIGR00096

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: NA

Molecular weight: Translated: 31348; Mature: 31348

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01296 RSMI

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHFGINA
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCE
RLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPGYHLVRTCREAGIRVVPLP
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEECC
GPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIEAEPRTLIFYESTHRLLDSLE
CCHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHH
DIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVKEDENRRKGEMVLIVEGHKAQEE
HHHHHHCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
DLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKYALEQQG
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAEDTRHTGLLLQHFGINA
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCE
RLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPGYHLVRTCREAGIRVVPLP
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEECC
GPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIEAEPRTLIFYESTHRLLDSLE
CCHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHH
DIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVKEDENRRKGEMVLIVEGHKAQEE
HHHHHHCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
DLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKYALEQQG
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796