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The map label for this gene is emrK [H]

Identifier: 209397981

GI number: 209397981

Start: 3314429

End: 3315592

Strand: Reverse

Name: emrK [H]

Synonym: ECH74115_3599

Alternate gene names: 209397981

Gene position: 3315592-3314429 (Counterclockwise)

Preceding gene: 209400136

Following gene: 209400997

Centisome position: 59.5

GC content: 41.84

Gene sequence:

>1164_bases
GTGGAACAGATTAATTCAAATAAAAAACATTCTAACAGAAGAAAATACTTTTCTTTATTGGCGATAGTTTTATTTATTGC
GTTTTCAGGTGCTTATGCCTATTGGTCAATGGAATTAGAAGACATGATTAGTACAGATGACGCCTATGTCACGGGGAATG
CAGATCCAATTTCTGCACAAGTCTCAGGTAGTGTCACTGTCGTTAATCATAAAGATACGAACTACGTTCGACAGGGTGAC
ATTTTAGTTTCACTGGATAAAACTGATGCCACTATCGCACTCAATAAAGCTAAAAATAATCTGGCAAATATTGTTCGGCA
AACGAATAAACTGTACTTACAGGATAAACAATACAGTGCCGAAGTCGCTTCAGCACGTATTCAGTATCAACAATCTTTAG
AAGATTATAACCGTCGTGTGCCTTTAGCGAAGCAGGGGGTTATTTCAAAAGAAACGCTGGAGCATACCAAAGATACGTTA
ATAAGTAGCAAAGCGGCATTGAATGCCGCTATCCAGGCTTATAAAGCGAATAAAGCTTTAGTAATGAACACACCATTAAA
CCGTCAGCCACAAGTCGTTGAAGCTGCGGATGCAACCAAAGAAGCCTGGTTGGCGCTTAAACGTACGGATATTAAGAGTC
CGGTTACCGGCTATATTGCCCAGAGAAGTGTTCAGGTCGGCGAAACAGTGAGTCCCGGACAATCGTTAATGGCTGTCGTA
CCGGCACGTCAAATGTGGGTTAATGCTAACTTTAAAGAAACACAACTCACGGATGTACGGATTGGTCAATCGGTCAATAT
TATCAGCGATCTTTATGGTGAGAATGTTGTGTTTCATGGCCGGGTGACAGGGATCAATATGGGAACCGGCAATGCGTTCT
CCTTATTACCTGCACAAAATGCGACAGGGAACTGGATCAAAATCGTTCAGCGTGTACCGGTTGAAGTTTCTCTTGATCCA
AAAGAACTCATGGAACACCCCTTGCGCATTGGTTTATCGATGACTGCAACTATTGATACGAAGAACGAAGACATTGCTGA
GATGCCTGAGCTGGCTTCAACCGTGACGTCCATGCCGGCTTATACCAGTAAGGCTTTAGTTATCGATACCAGTCCGATAG
AAAAAGAAATTAGCAACATTATTTCGCATAATGGACAACTTTAA

Upstream 100 bases:

>100_bases
CCAGGGATAATGTGCAACACATGCACTGTGTTTGATATGAAGAATGAATACTCTTTTCATTCAATTCATAAATTTCATCT
ATGCGAAATGAGAGATAATA

Downstream 100 bases:

>100_bases
TGGCAATCACTAAATCAACTCCGGCACCATTAACCGGTGGGACGTTATGGTGCGTCACTATTGCATTGTCATTAGCGACG
TTTATGCAAATGTTGGATTC

Product: drug resistance MFS transporter, membrane fusion protein (MFP) subunit EmrK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL

Sequences:

>Translated_387_residues
MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL
>Mature_387_residues
MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=387, Percent_Identity=99.7416020671835, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1789041, Length=378, Percent_Identity=49.4708994708995, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1790519, Length=324, Percent_Identity=27.7777777777778, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=28.2958199356913, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789637, Length=310, Percent_Identity=26.4516129032258, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790024, Length=238, Percent_Identity=22.6890756302521, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42600; Mature: 42600

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCEEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
YTSKALVIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCEEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
YTSKALVIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]